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The map label for this gene is dpiA [H]

Identifier: 82543067

GI number: 82543067

Start: 504271

End: 504951

Strand: Direct

Name: dpiA [H]

Synonym: SBO_0485

Alternate gene names: 82543067

Gene position: 504271-504951 (Clockwise)

Preceding gene: 82543057

Following gene: 82543069

Centisome position: 11.16

GC content: 50.37

Gene sequence:

>681_bases
ATGACAGCTCCATTAACCCTATTGATCGTTGAGGACGAAACGCCGCTGGCAGAGATGCATGCGGAATATATTCGTCACAT
TCCCGGATTCAGTCAGATATTACTGGCGGGAAACCTGGCGCAGGCCCGAATGATGATCGAGCGTTTTAAGCCGGGGCTAA
TCTTGCTCGATAACTATCTTCCTGACGGCAGAGGGATTAATTTATTGCATGAACTGGTGCAGGCGCATTATCCCGGCGAC
GTGGTGTTTACCACTGCAGCCAGCGATATGGAAACGGTGTCTGAAGCCGTACGTTGTGGTGTATTTGATTATCTCATTAA
GCCCATTGCCTATGAACGGCTGGGGCAAACGCTAACCCGTTTCCGCCAGCGTAAACATATGCTGGAAAGTATTGATAGCG
CCAGCCAGAAGCAAATTGATGAGATGTTTAATGCCTATGCTCGCGGTGAACCTAAAGACGAACTGCCGACCGGCATTGAT
CCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACT
GACCATCAGCCGCACGTCTGCCAGACGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACG
GCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGA

Upstream 100 bases:

>100_bases
ATTGCCAGCTACGTAACGCGCTGCGGTGGTGTTATCACTCTCGAAGATAATGATCCCTGCGGTACCTTATTTTCAATCTA
TATTCCGAAAGTGAAACCTA

Downstream 100 bases:

>100_bases
CATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCTGTTCCTGGCACCATC
AGCTCTGTAGCAACGATAAC

Product: two-component response regulator DpiA

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGD
VVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG

Sequences:

>Translated_226_residues
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGD
VVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
>Mature_225_residues
TAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDV
VFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDP
LTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=226, Percent_Identity=99.5575221238938, Blast_Score=465, Evalue=1e-133,
Organism=Escherichia coli, GI1790566, Length=220, Percent_Identity=31.8181818181818, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=32.824427480916, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25440; Mature: 25309

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYL
CCCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCC
PDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTR
CCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDPLTLNAVRKLFKEPGVQHTA
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
TAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARMMIERFKPGLILLDNYL
CCCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCC
PDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVFDYLIKPIAYERLGQTLTR
CCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDPLTLNAVRKLFKEPGVQHTA
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGRPQRIYHSG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]