Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 78779748

GI number: 78779748

Start: 1281851

End: 1282888

Strand: Reverse

Name: pheS

Synonym: PMT9312_1364

Alternate gene names: 78779748

Gene position: 1282888-1281851 (Counterclockwise)

Preceding gene: 78779750

Following gene: 78779747

Centisome position: 75.06

GC content: 32.95

Gene sequence:

>1038_bases
ATGAAAGCAATACCTACTTTTACTATATCAGTGCGTCAAATTGAATCATTAAGTCAAATTGAGGAAAAACTAAATAATCT
TTCTCTAACAGCAAAAAATAATATAGATAATGCTTCTACTCATGAAGAACTGGATAAATTAAGAGTTTCCTTACTAGGAA
AAAAAGGTGATTTGTCAATAATTCTGAAAATAATGGGTCAATTATCTGCTGCCGAAAGACCAATTGTTGGCCAAAAAGCA
AATTTTATAAAGATAAATTTGCAAGATTTACTTAATAAAAGAAAAAATCAATTAAATAATGAAGCCTTAGATCAGCAGAT
CAAAAATGAAAAAATTGATGTAACTATTCCTTCAATTGGAACACCTCCTGGAAGCAAGCATCCCTTAATTTCAACTCAAG
ATGAGATCATAGATATTTTTTGTGGTTTAGGTTATACCGTTGAAAGTGGTCCTGAAATAGAAAGTGATTTTTATAATTTT
GAGTCTCTAAATATACCCAAAAATCATCCTGCAAGAGATATGCAAGATACTTTCTATTTAGATGAAAATCGACTTTTGAG
GACCCATACTTCTCCCGTTCAGATAAGGTACTTAGAAAAAAATCCTCCTCCAGTAAGAATTATTGCTCCTGGGAGAGTTT
ATAGGAGAGATGCTGTAGATGCTACCCACTCTCCAGTATTTAATCAGGTTGAGGTTTTATGCATAGATCAAGATATAAAT
TTTAGCCACCTAAGAGGGACAGTTCTTACTTTTTTAAAGACTTTTTTTGGAGATATTCCTGTAAGATTTAGAGCTAGTTA
TTTCCCATTCACTGAACCCTCGGCCGAAGTGGACGTTCAATGGAAAGGTAAATGGTTAGAAGTAATGGGCTGCGGAATGG
TAGATCCCAAGGTTTTGGAGAAATTAGGAATAGATTCTGAAAAATGGACTGGATTTGCAGCTGGATTAGGCGTTGAAAGA
TTTTGTATGGTAAGACATCAGATTGACGATATTAGAAAATTTTATACAAATGATCTTAGATTCTTAGAACAGTTTTAA

Upstream 100 bases:

>100_bases
CTCTTAATCCCTCTGCAAAAACACCATCATCATTACTAATTAATATATTTAACGATTCCATTATTCAATACATTTTATTT
ATGGACGATATTTAAGTAAG

Downstream 100 bases:

>100_bases
TAGAATTTAAGTTATAAATTATGATTGTCCAGCAAATTAGTTTAAAATAGTTGTAGTTTTAGTTTTTTGATTGGTACTTA
AAGCAGGACTAATAGTTAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKAIPTFTISVRQIESLSQIEEKLNNLSLTAKNNIDNASTHEELDKLRVSLLGKKGDLSIILKIMGQLSAAERPIVGQKA
NFIKINLQDLLNKRKNQLNNEALDQQIKNEKIDVTIPSIGTPPGSKHPLISTQDEIIDIFCGLGYTVESGPEIESDFYNF
ESLNIPKNHPARDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCIDQDIN
FSHLRGTVLTFLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSEKWTGFAAGLGVER
FCMVRHQIDDIRKFYTNDLRFLEQF

Sequences:

>Translated_345_residues
MKAIPTFTISVRQIESLSQIEEKLNNLSLTAKNNIDNASTHEELDKLRVSLLGKKGDLSIILKIMGQLSAAERPIVGQKA
NFIKINLQDLLNKRKNQLNNEALDQQIKNEKIDVTIPSIGTPPGSKHPLISTQDEIIDIFCGLGYTVESGPEIESDFYNF
ESLNIPKNHPARDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCIDQDIN
FSHLRGTVLTFLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSEKWTGFAAGLGVER
FCMVRHQIDDIRKFYTNDLRFLEQF
>Mature_345_residues
MKAIPTFTISVRQIESLSQIEEKLNNLSLTAKNNIDNASTHEELDKLRVSLLGKKGDLSIILKIMGQLSAAERPIVGQKA
NFIKINLQDLLNKRKNQLNNEALDQQIKNEKIDVTIPSIGTPPGSKHPLISTQDEIIDIFCGLGYTVESGPEIESDFYNF
ESLNIPKNHPARDMQDTFYLDENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCIDQDIN
FSHLRGTVLTFLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLEKLGIDSEKWTGFAAGLGVER
FCMVRHQIDDIRKFYTNDLRFLEQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=229, Percent_Identity=32.7510917030568, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI4758340, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788007, Length=324, Percent_Identity=51.5432098765432, Blast_Score=334, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI32566635, Length=364, Percent_Identity=30.4945054945055, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=285, Percent_Identity=29.1228070175439, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=285, Percent_Identity=29.1228070175439, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=260, Percent_Identity=31.5384615384615, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6325304, Length=88, Percent_Identity=42.0454545454545, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137424, Length=280, Percent_Identity=29.2857142857143, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18858079, Length=279, Percent_Identity=29.3906810035842, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PROM9 (Q319M1)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397860.1
- ProteinModelPortal:   Q319M1
- SMR:   Q319M1
- STRING:   Q319M1
- GeneID:   3766176
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1364
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   PMAR74546:PMT9312_1364-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39308; Mature: 39308

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIPTFTISVRQIESLSQIEEKLNNLSLTAKNNIDNASTHEELDKLRVSLLGKKGDLSI
CCCCCEEEEEHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ILKIMGQLSAAERPIVGQKANFIKINLQDLLNKRKNQLNNEALDQQIKNEKIDVTIPSIG
HHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCC
TPPGSKHPLISTQDEIIDIFCGLGYTVESGPEIESDFYNFESLNIPKNHPARDMQDTFYL
CCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCHHEEE
DENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCIDQDIN
CCCCEEEECCCCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCCC
FSHLRGTVLTFLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLE
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHH
KLGIDSEKWTGFAAGLGVERFCMVRHQIDDIRKFYTNDLRFLEQF
HHCCCCHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAIPTFTISVRQIESLSQIEEKLNNLSLTAKNNIDNASTHEELDKLRVSLLGKKGDLSI
CCCCCEEEEEHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ILKIMGQLSAAERPIVGQKANFIKINLQDLLNKRKNQLNNEALDQQIKNEKIDVTIPSIG
HHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCC
TPPGSKHPLISTQDEIIDIFCGLGYTVESGPEIESDFYNFESLNIPKNHPARDMQDTFYL
CCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCCCHHEEE
DENRLLRTHTSPVQIRYLEKNPPPVRIIAPGRVYRRDAVDATHSPVFNQVEVLCIDQDIN
CCCCEEEECCCCEEEEEEECCCCCEEEECCCCHHHHHCCCCCCCCCCCCEEEEEEECCCC
FSHLRGTVLTFLKTFFGDIPVRFRASYFPFTEPSAEVDVQWKGKWLEVMGCGMVDPKVLE
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHH
KLGIDSEKWTGFAAGLGVERFCMVRHQIDDIRKFYTNDLRFLEQF
HHCCCCHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA