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The map label for this gene is argF

Identifier: 78779741

GI number: 78779741

Start: 1274731

End: 1275657

Strand: Reverse

Name: argF

Synonym: PMT9312_1357

Alternate gene names: 78779741

Gene position: 1275657-1274731 (Counterclockwise)

Preceding gene: 78779742

Following gene: 78779740

Centisome position: 74.63

GC content: 32.25

Gene sequence:

>927_bases
ATGGTAAAACCAAATAAGCTTGCTAATAATAATTATCTATCAAGCTTTGATATGACAACTGATGAGTTTATTAATATTTT
AGACCTTGCAAAAAATTTTAAAAATAAAAAGATAAATATTGATCCTAACAATAAAGTTTTAGGATTAATATTTGATAAGT
CATCAACACGAACAAGAGTTAGTTTTCAAGTCGCGATGTCACGACTTGGTGGAACCACTATCGACCTAAATCCGATTACA
TCCCAAATAGGAAGAGGAGAGCCCATTAAAGATACTGCTAGAGTTCTAAGTAGATATTGCGATGTTATTGCAATTAGAAC
TTTTAATCATTCAGATCTAGAAGAATATGCAAAATGGTCTACGATACCAGTAATTAATGCTCTTACAGATTTAGAACATC
CCTGCCAAGCTCTAGCAGATTTTTTGACAATTCAAGAGGAGTTTATCGATTTTAGAAATGTAGTTTTGACATTTATAGGT
GATGGTAATAACGTCGCAAACTCCCTTATTCTATGTGGAGCATTACTAGGAGTAGAAGTTAGAATTGCATGCCCAAGAGG
TTATGAACCAAATGTCTCGGTTATTAACAAAGCACTTGAAATTTACAAAAATAAGAATTTGTTGAAAATCATTCATGATC
CTTATGATGCTGTATTAGGGGCAAATGTTCTTTATACAGATGTTTGGTCATCTATGGGAGAGGAAAATCAAAAAGAAGAT
AAAGATAAAGATTTTAAAGGATTTACTATTAATAGCAGTTTAGTAAGAAAGGCAAATAAAGATGCAATTATTCTCCATTG
TCTGCCAGCATATAGGGGTAAAGAAATTTCAGCAGAAGTTTTTGAGAGTAAAATTAGTAGAATTTTTAAGCAGGCAGAAA
ATAGGTTGCATGTCCAGCAAGCTCTTTTGGCAGGATTGTTAAGTTAA

Upstream 100 bases:

>100_bases
ATTCTTGAAGAAGAAGTTATTGAGGGTGAGGAGTTGAAAAATCTGCTTTCAGAATCTAAGTTACCTGCATAGTAGAATCT
AGATCTCACTAGAATTATTC

Downstream 100 bases:

>100_bases
ATAGTTGCAATAATTCCTTTTAAGAGGTACAAATGTATTAGTTTGCATTTGCTTTAGAGATGATTCCTTCTGAAAAGACT
CTTACTGAGCCACAAAATGA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MVKPNKLANNNYLSSFDMTTDEFINILDLAKNFKNKKINIDPNNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPIT
SQIGRGEPIKDTARVLSRYCDVIAIRTFNHSDLEEYAKWSTIPVINALTDLEHPCQALADFLTIQEEFIDFRNVVLTFIG
DGNNVANSLILCGALLGVEVRIACPRGYEPNVSVINKALEIYKNKNLLKIIHDPYDAVLGANVLYTDVWSSMGEENQKED
KDKDFKGFTINSSLVRKANKDAIILHCLPAYRGKEISAEVFESKISRIFKQAENRLHVQQALLAGLLS

Sequences:

>Translated_308_residues
MVKPNKLANNNYLSSFDMTTDEFINILDLAKNFKNKKINIDPNNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPIT
SQIGRGEPIKDTARVLSRYCDVIAIRTFNHSDLEEYAKWSTIPVINALTDLEHPCQALADFLTIQEEFIDFRNVVLTFIG
DGNNVANSLILCGALLGVEVRIACPRGYEPNVSVINKALEIYKNKNLLKIIHDPYDAVLGANVLYTDVWSSMGEENQKED
KDKDFKGFTINSSLVRKANKDAIILHCLPAYRGKEISAEVFESKISRIFKQAENRLHVQQALLAGLLS
>Mature_308_residues
MVKPNKLANNNYLSSFDMTTDEFINILDLAKNFKNKKINIDPNNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPIT
SQIGRGEPIKDTARVLSRYCDVIAIRTFNHSDLEEYAKWSTIPVINALTDLEHPCQALADFLTIQEEFIDFRNVVLTFIG
DGNNVANSLILCGALLGVEVRIACPRGYEPNVSVINKALEIYKNKNLLKIIHDPYDAVLGANVLYTDVWSSMGEENQKED
KDKDFKGFTINSSLVRKANKDAIILHCLPAYRGKEISAEVFESKISRIFKQAENRLHVQQALLAGLLS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=38.3870967741935, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=29.8412698412698, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=35.6707317073171, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=35.7575757575758, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI48994908, Length=360, Percent_Identity=24.1666666666667, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=27.3015873015873, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=331, Percent_Identity=26.8882175226586, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=307, Percent_Identity=35.8306188925081, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322331, Length=269, Percent_Identity=31.5985130111524, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=29.5081967213115, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_PROM9 (Q319M8)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397853.1
- HSSP:   P96108
- ProteinModelPortal:   Q319M8
- SMR:   Q319M8
- STRING:   Q319M8
- GeneID:   3766169
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1357
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   FTVAMYQ
- ProtClustDB:   PRK00779
- BioCyc:   PMAR74546:PMT9312_1357-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34527; Mature: 34527

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 106-106 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKPNKLANNNYLSSFDMTTDEFINILDLAKNFKNKKINIDPNNKVLGLIFDKSSTRTRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHH
SFQVAMSRLGGTTIDLNPITSQIGRGEPIKDTARVLSRYCDVIAIRTFNHSDLEEYAKWS
HHHHHHHHHCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHCCC
TIPVINALTDLEHPCQALADFLTIQEEFIDFRNVVLTFIGDGNNVANSLILCGALLGVEV
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCEEE
RIACPRGYEPNVSVINKALEIYKNKNLLKIIHDPYDAVLGANVLYTDVWSSMGEENQKED
EEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHHHHCCCCCHHC
KDKDFKGFTINSSLVRKANKDAIILHCLPAYRGKEISAEVFESKISRIFKQAENRLHVQQ
CCCCCCCEEECHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALLAGLLS
HHHHHHCC
>Mature Secondary Structure
MVKPNKLANNNYLSSFDMTTDEFINILDLAKNFKNKKINIDPNNKVLGLIFDKSSTRTRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCHHH
SFQVAMSRLGGTTIDLNPITSQIGRGEPIKDTARVLSRYCDVIAIRTFNHSDLEEYAKWS
HHHHHHHHHCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHCEEEEEECCCCCHHHHHCCC
TIPVINALTDLEHPCQALADFLTIQEEFIDFRNVVLTFIGDGNNVANSLILCGALLGVEV
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCEEE
RIACPRGYEPNVSVINKALEIYKNKNLLKIIHDPYDAVLGANVLYTDVWSSMGEENQKED
EEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHHHHCCCCCHHC
KDKDFKGFTINSSLVRKANKDAIILHCLPAYRGKEISAEVFESKISRIFKQAENRLHVQQ
CCCCCCCEEECHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALLAGLLS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA