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The map label for this gene is rluF [H]

Identifier: 78779587

GI number: 78779587

Start: 1125626

End: 1126348

Strand: Reverse

Name: rluF [H]

Synonym: PMT9312_1204

Alternate gene names: 78779587

Gene position: 1126348-1125626 (Counterclockwise)

Preceding gene: 78779589

Following gene: 78779586

Centisome position: 65.9

GC content: 31.26

Gene sequence:

>723_bases
ATGGCTACTAGAATAAATAAATATTTAAGTGAAGTAGGTTATTGCTCTAGAAGAAAAGCAGATAGACTGATTGAAGAAGG
AAAAGTAACTATTAATGGTAAGATTCCTGAAATAGGTGCCAAGGTGGAGGATGGTGATCAAGTAGAAGTTGAAGGTCAAA
GAATAAAAAAATCTACGAAACAAAAAAACATATATTTAGCCTTTAATAAACCTATTGGAGTCGTTTGCACAACTGATAGG
GGCGTAGAACCAGACAATATCATAGATTTCATTAAATATCCGAAAAGGATCTTCCCTATTGGAAGATTAGATAAGTCTAG
TGAGGGGTTGATTTTTTTAACCAACGATGGAGATATTGTAAATAAAATACTCAGAGCAAGAAATAATCATGAAAAAGAAT
ACATCGTAAGCGTTAATCGTCCAATTAATAGAGAATTTATTCAAACCATGAGTAATGGAGTTGAAATACTAGAAACCAAA
ACTAAGAATTGTTTTGTAAAAAAATTAGGGCCAAAAAAATTTAAAATCATACTCACTCAAGGACTTAACCGTCAGATTAG
GAGAATGTGTGAGTCCTTAGGCTACAGAGTACAATCATTAAAGCGTGTAAGGATTATGAATATTAAGCTAGATTTACCAA
TAGGAAAATATCGCGAATTTACCAAAGAAGAAATCTCAGATTTAAATGCATTACTGAAAAACTCTTCAAAAATATTTGAA
TAA

Upstream 100 bases:

>100_bases
TGAAATTATTGAAAATGAAGTTTAAATTGTTGACGAAATTGTCCATTTTCTTTTCGCAATTTGTCCTCATATTTAAAATA
AATAAAATGTAAATTGAAAA

Downstream 100 bases:

>100_bases
TCGAAAACCTTCCTCTTTTGATTTGATAAGTTAAATTTACTCACTATTTCCCCACATAATGTGGAGAAAAATATTAACCC
ATTAAAAAAAACTGCCTTGG

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MATRINKYLSEVGYCSRRKADRLIEEGKVTINGKIPEIGAKVEDGDQVEVEGQRIKKSTKQKNIYLAFNKPIGVVCTTDR
GVEPDNIIDFIKYPKRIFPIGRLDKSSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINREFIQTMSNGVEILETK
TKNCFVKKLGPKKFKIILTQGLNRQIRRMCESLGYRVQSLKRVRIMNIKLDLPIGKYREFTKEEISDLNALLKNSSKIFE

Sequences:

>Translated_240_residues
MATRINKYLSEVGYCSRRKADRLIEEGKVTINGKIPEIGAKVEDGDQVEVEGQRIKKSTKQKNIYLAFNKPIGVVCTTDR
GVEPDNIIDFIKYPKRIFPIGRLDKSSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINREFIQTMSNGVEILETK
TKNCFVKKLGPKKFKIILTQGLNRQIRRMCESLGYRVQSLKRVRIMNIKLDLPIGKYREFTKEEISDLNALLKNSSKIFE
>Mature_239_residues
ATRINKYLSEVGYCSRRKADRLIEEGKVTINGKIPEIGAKVEDGDQVEVEGQRIKKSTKQKNIYLAFNKPIGVVCTTDRG
VEPDNIIDFIKYPKRIFPIGRLDKSSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINREFIQTMSNGVEILETKT
KNCFVKKLGPKKFKIILTQGLNRQIRRMCESLGYRVQSLKRVRIMNIKLDLPIGKYREFTKEEISDLNALLKNSSKIFE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=57.6271186440678, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1787524, Length=257, Percent_Identity=30.3501945525292, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27638; Mature: 27506

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRINKYLSEVGYCSRRKADRLIEEGKVTINGKIPEIGAKVEDGDQVEVEGQRIKKSTK
CCCHHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECHHHHHHHHH
QKNIYLAFNKPIGVVCTTDRGVEPDNIIDFIKYPKRIFPIGRLDKSSEGLIFLTNDGDIV
HCEEEEEECCCEEEEEECCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINREFIQTMSNGVEILETKTKNCFVKKLGPKKFKIILTQ
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHEEECCCCHHHHCCCCCCEEEEEEEC
GLNRQIRRMCESLGYRVQSLKRVRIMNIKLDLPIGKYREFTKEEISDLNALLKNSSKIFE
CCCHHHHHHHHHHCHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
ATRINKYLSEVGYCSRRKADRLIEEGKVTINGKIPEIGAKVEDGDQVEVEGQRIKKSTK
CCHHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECHHHHHHHHH
QKNIYLAFNKPIGVVCTTDRGVEPDNIIDFIKYPKRIFPIGRLDKSSEGLIFLTNDGDIV
HCEEEEEECCCEEEEEECCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINREFIQTMSNGVEILETKTKNCFVKKLGPKKFKIILTQ
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHEEECCCCHHHHCCCCCCEEEEEEEC
GLNRQIRRMCESLGYRVQSLKRVRIMNIKLDLPIGKYREFTKEEISDLNALLKNSSKIFE
CCCHHHHHHHHHHCHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]