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The map label for this gene is nth [H]

Identifier: 78779194

GI number: 78779194

Start: 753044

End: 753697

Strand: Reverse

Name: nth [H]

Synonym: PMT9312_0810

Alternate gene names: 78779194

Gene position: 753697-753044 (Counterclockwise)

Preceding gene: 78779197

Following gene: 78779193

Centisome position: 44.1

GC content: 33.18

Gene sequence:

>654_bases
ATGAGAAAGGCTGAAAGAGCAGAAATAATACGCAAGGAGCTCAAAAATCTATATCCATCTCCTCCAATACCTCTTGATCA
TTCAAATGCGTATACACTTCTCGTCGCCGTGGTTTTAAGTGCTCAATCAACAGATAAGAAAGTTAATGAATTAACCAAAA
ACTTATTTAAGGTAGCAGATAATCCAGAAAAGATGGTGAATCTAGGCATTAATGGTATTTATGAATACATAAAATTTTTA
GGTCTATCTAATCAAAAATCAAAGAATATCTATAACCTTTCTAAACTCTTGATTGAGAAGCATAAAAGTATAGTCCCAAA
TACTTTTGAGAAGCTTGAATCTCTTCCAGGGGTAGGTCATAAAACAGCATCAGTAGTAATGTCACAAGTATTTAAAATCC
CCTCATTCCCTGTTGATACTCATATACACCGGTTGTCACAAAGATGGGGTCTATCAAATGGAGATAGCGTGGTTCAAACA
GAAAAAGACCTAAAAAAAATATTTCCTGTTAATGAATGGAATACTTTACATTTGCAAATAATCTTTTTTGGAAGGGAATA
CTGTACCGCAAGAGGCTGTGATGGAACAAAATGTTATTTATGTCGTACTCTTTATCCCAAAAGAAAAAAAAAATTTATAT
GTAAAAAGCCCTAA

Upstream 100 bases:

>100_bases
TCACAAGAGTCTTCTTCTTTAAATATATTAAATTAGCAGAAAATATTAACCTAAAATATCTCTTGTATAATTTTATACAC
GATTCAATTTGTCAAATTCA

Downstream 100 bases:

>100_bases
TAAATTTATATAATAATTGAATTAATTTTTAAATCATGAGAATAGCAATTACTGGTGCATCGGGGAAAACAGGTTTTAGA
ATTTCTGAAGAGGCAGTTAA

Product: putative endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRKAERAEIIRKELKNLYPSPPIPLDHSNAYTLLVAVVLSAQSTDKKVNELTKNLFKVADNPEKMVNLGINGIYEYIKFL
GLSNQKSKNIYNLSKLLIEKHKSIVPNTFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNEWNTLHLQIIFFGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP

Sequences:

>Translated_217_residues
MRKAERAEIIRKELKNLYPSPPIPLDHSNAYTLLVAVVLSAQSTDKKVNELTKNLFKVADNPEKMVNLGINGIYEYIKFL
GLSNQKSKNIYNLSKLLIEKHKSIVPNTFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNEWNTLHLQIIFFGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
>Mature_217_residues
MRKAERAEIIRKELKNLYPSPPIPLDHSNAYTLLVAVVLSAQSTDKKVNELTKNLFKVADNPEKMVNLGINGIYEYIKFL
GLSNQKSKNIYNLSKLLIEKHKSIVPNTFEKLESLPGVGHKTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQT
EKDLKKIFPVNEWNTLHLQIIFFGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=191, Percent_Identity=42.4083769633508, Blast_Score=151, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17554540, Length=201, Percent_Identity=25.8706467661692, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=25.8241758241758, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=184, Percent_Identity=27.7173913043478, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24850; Mature: 24850

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAERAEIIRKELKNLYPSPPIPLDHSNAYTLLVAVVLSAQSTDKKVNELTKNLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
NPEKMVNLGINGIYEYIKFLGLSNQKSKNIYNLSKLLIEKHKSIVPNTFEKLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
KTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQTEKDLKKIFPVNEWNTLHLQI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
IFFGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
EEECHHHHHCCCCCCCCEEHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure
MRKAERAEIIRKELKNLYPSPPIPLDHSNAYTLLVAVVLSAQSTDKKVNELTKNLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
NPEKMVNLGINGIYEYIKFLGLSNQKSKNIYNLSKLLIEKHKSIVPNTFEKLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCH
KTASVVMSQVFKIPSFPVDTHIHRLSQRWGLSNGDSVVQTEKDLKKIFPVNEWNTLHLQI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
IFFGREYCTARGCDGTKCYLCRTLYPKRKKKFICKKP
EEECHHHHHCCCCCCCCEEHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA