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The map label for this gene is hisS

Identifier: 78779008

GI number: 78779008

Start: 588575

End: 589855

Strand: Direct

Name: hisS

Synonym: PMT9312_0623

Alternate gene names: 78779008

Gene position: 588575-589855 (Clockwise)

Preceding gene: 78779006

Following gene: 78779009

Centisome position: 34.44

GC content: 32.47

Gene sequence:

>1281_bases
TTGAATAACTTAAAAAATCTTAGAGGAACAGTAGATCTATTGCCTGATCAATTAATAAAGTGGCAAAACGTTGAGAAAAT
TGTATTGGAGCAGCTTGCAAGAGCATCCATCAAAGAAATAAGAACACCAATATTGGAAATGACTGAATTATTTATAAGAG
GAATTGGTGAAGGAACTGATGTTGTTAGTAAGGAAATGTACACATTTCTTGATAGAGGGGAGAGATCCTGCACTCTTAGG
CCTGAAGGAACCGCCTCCGTCGCACGAGCGTTAATACAAAATGGAATATCGTCTAATCCTTTTCAAAAACTATGGTACAT
GGGGCCTATGTTTCGATATGAAAGACCTCAAGCAGGAAGGCAAAGACAGTTTCATCAGTTAGGCGTTGAGTTTATAGGAT
ACGAATCAGTGAGAAGTGATGTTGAAATTATTGCTTTAGCTTGGGATATCTTAGGGAAATTAGGAATTAAAGAACTTAAT
CTTGAAATAAATAGCTTGGGTGATATTGATGATAGATTAAATTTTCAAAAATCTTTTTTGAAGTGGCTAGAAAAAAATAA
AGATTCTTTAGATTTAGATTCTCAGAATAGAATCAATAAAAATCCTTTAAGGATTTTAGACTCTAAGAATATGCAAACAA
AGAAAGCGCTTGAAAACGCTCCAATATTATTTAATTTTTTGTCTGAAAAAAGTCATAAAAGATATTCAGATTTAAAAAAA
CAATTGAATGTTTTACAAATACCCTACGTGGAAAACTTTAATCTAGTCAGGGGTCTGGATTATTACACCCATACCGCCTT
CGAAATCACTAGTTGTGCTCTAGGCTCCCAAGCTACAGTTTGCGGAGGAGGAAGATATGACAATTTAATAAAACAAATGG
GTGGGCCAAATACTCCAGCAATTGGATTTGCTATTGGTTTAGAAAGATTAATTTTACTAGCGGGAAAAGATCTTGAAGTT
CCAAGGAATACTGATATTTATATTATTAATCAAGGCTTAATTGCTGAATCATTAGCTATGGATTTATCTAGAAAATTAAG
AAATTATGATTTGTTAGTTGAATTGGATTTAAGTGGGGCCTCATTCTCTAAACAATTTAAAAAGGCAAATAAACTAAAAT
CTAAAAGTATTATTGTTATTGGTGATGATGAGGCGGCTAATAAAGAGTTTATTATAAGGCTCTTTGATCATTCAGGTAAT
GCGAATAAAGAAGAGGTTATATCTTTTGAGAATGATATTAAATTAGAAAAGTGGCTAAAAATTAACTTACTTTCAAAGTG
A

Upstream 100 bases:

>100_bases
AATAGTTTTTCTAAATGTTAAATTAAAATTGTCTCACAGAACAAACAATCAAAGATTAATTTCTACTTGAATTAAAAATT
TTGAATTATTTTCTAGAACT

Downstream 100 bases:

>100_bases
TCCTCTTGAAGTTAATGATAATTTGTCTTTTTATATTTATTTTCCTTTATTTAAGAAATTTTCTTAAATTAAATAGGGAG
GGGAAAGTTTTTAAGGTTAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNNLKNLRGTVDLLPDQLIKWQNVEKIVLEQLARASIKEIRTPILEMTELFIRGIGEGTDVVSKEMYTFLDRGERSCTLR
PEGTASVARALIQNGISSNPFQKLWYMGPMFRYERPQAGRQRQFHQLGVEFIGYESVRSDVEIIALAWDILGKLGIKELN
LEINSLGDIDDRLNFQKSFLKWLEKNKDSLDLDSQNRINKNPLRILDSKNMQTKKALENAPILFNFLSEKSHKRYSDLKK
QLNVLQIPYVENFNLVRGLDYYTHTAFEITSCALGSQATVCGGGRYDNLIKQMGGPNTPAIGFAIGLERLILLAGKDLEV
PRNTDIYIINQGLIAESLAMDLSRKLRNYDLLVELDLSGASFSKQFKKANKLKSKSIIVIGDDEAANKEFIIRLFDHSGN
ANKEEVISFENDIKLEKWLKINLLSK

Sequences:

>Translated_426_residues
MNNLKNLRGTVDLLPDQLIKWQNVEKIVLEQLARASIKEIRTPILEMTELFIRGIGEGTDVVSKEMYTFLDRGERSCTLR
PEGTASVARALIQNGISSNPFQKLWYMGPMFRYERPQAGRQRQFHQLGVEFIGYESVRSDVEIIALAWDILGKLGIKELN
LEINSLGDIDDRLNFQKSFLKWLEKNKDSLDLDSQNRINKNPLRILDSKNMQTKKALENAPILFNFLSEKSHKRYSDLKK
QLNVLQIPYVENFNLVRGLDYYTHTAFEITSCALGSQATVCGGGRYDNLIKQMGGPNTPAIGFAIGLERLILLAGKDLEV
PRNTDIYIINQGLIAESLAMDLSRKLRNYDLLVELDLSGASFSKQFKKANKLKSKSIIVIGDDEAANKEFIIRLFDHSGN
ANKEEVISFENDIKLEKWLKINLLSK
>Mature_426_residues
MNNLKNLRGTVDLLPDQLIKWQNVEKIVLEQLARASIKEIRTPILEMTELFIRGIGEGTDVVSKEMYTFLDRGERSCTLR
PEGTASVARALIQNGISSNPFQKLWYMGPMFRYERPQAGRQRQFHQLGVEFIGYESVRSDVEIIALAWDILGKLGIKELN
LEINSLGDIDDRLNFQKSFLKWLEKNKDSLDLDSQNRINKNPLRILDSKNMQTKKALENAPILFNFLSEKSHKRYSDLKK
QLNVLQIPYVENFNLVRGLDYYTHTAFEITSCALGSQATVCGGGRYDNLIKQMGGPNTPAIGFAIGLERLILLAGKDLEV
PRNTDIYIINQGLIAESLAMDLSRKLRNYDLLVELDLSGASFSKQFKKANKLKSKSIIVIGDDEAANKEFIIRLFDHSGN
ANKEEVISFENDIKLEKWLKINLLSK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=397, Percent_Identity=45.5919395465995, Blast_Score=348, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6325290, Length=455, Percent_Identity=22.4175824175824, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48477; Mature: 48477

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKNLRGTVDLLPDQLIKWQNVEKIVLEQLARASIKEIRTPILEMTELFIRGIGEGTD
CCCHHHHCCHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VVSKEMYTFLDRGERSCTLRPEGTASVARALIQNGISSNPFQKLWYMGPMFRYERPQAGR
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHCCCCCCHH
QRQFHQLGVEFIGYESVRSDVEIIALAWDILGKLGIKELNLEINSLGDIDDRLNFQKSFL
HHHHHHHCHHHHCHHHHHCCHHHHHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHH
KWLEKNKDSLDLDSQNRINKNPLRILDSKNMQTKKALENAPILFNFLSEKSHKRYSDLKK
HHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
QLNVLQIPYVENFNLVRGLDYYTHTAFEITSCALGSQATVCGGGRYDNLIKQMGGPNTPA
HCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHCCCCCCCH
IGFAIGLERLILLAGKDLEVPRNTDIYIINQGLIAESLAMDLSRKLRNYDLLVELDLSGA
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCC
SFSKQFKKANKLKSKSIIVIGDDEAANKEFIIRLFDHSGNANKEEVISFENDIKLEKWLK
HHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCHHHEEE
INLLSK
EEECCC
>Mature Secondary Structure
MNNLKNLRGTVDLLPDQLIKWQNVEKIVLEQLARASIKEIRTPILEMTELFIRGIGEGTD
CCCHHHHCCHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VVSKEMYTFLDRGERSCTLRPEGTASVARALIQNGISSNPFQKLWYMGPMFRYERPQAGR
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHCCCCCCHH
QRQFHQLGVEFIGYESVRSDVEIIALAWDILGKLGIKELNLEINSLGDIDDRLNFQKSFL
HHHHHHHCHHHHCHHHHHCCHHHHHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHHHH
KWLEKNKDSLDLDSQNRINKNPLRILDSKNMQTKKALENAPILFNFLSEKSHKRYSDLKK
HHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
QLNVLQIPYVENFNLVRGLDYYTHTAFEITSCALGSQATVCGGGRYDNLIKQMGGPNTPA
HCCEEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHCCCCCCCH
IGFAIGLERLILLAGKDLEVPRNTDIYIINQGLIAESLAMDLSRKLRNYDLLVELDLSGA
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCC
SFSKQFKKANKLKSKSIIVIGDDEAANKEFIIRLFDHSGNANKEEVISFENDIKLEKWLK
HHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCHHHEEE
INLLSK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA