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The map label for this gene is menD

Identifier: 78778992

GI number: 78778992

Start: 570590

End: 572353

Strand: Direct

Name: menD

Synonym: PMT9312_0607

Alternate gene names: 78778992

Gene position: 570590-572353 (Clockwise)

Preceding gene: 78778989

Following gene: 78778993

Centisome position: 33.38

GC content: 30.39

Gene sequence:

>1764_bases
ATGACATCATCTATTGAATGCAAAAATTTTCTTAGAAGTTTACAACTTTTAAATCTTCTTATAAAAATAGGAGTTCAAAA
TTTAATAGTATGTCCTGGTAGTAGATCAGCGCCTTTAGCAATAGCTGCTGGAGAATTAAATAAATTAGGACTGGTAAATA
TTTTTAATTCAATAGATGAGAGATCTGCTGGATTCCACTCTCTTGGGATTTCTGCTGCATCAGGTAATCTTTCTTTAGTT
ATTACCACTTCCGGAACTGCCGTAAGTAATTTATTGCCAGCAGCAGTTGAGGCTGACAGATCTTGTAAAGGTATTATATT
TCTTACTGCTGATAGGCCTCTAAGATTAAAAGATTGTGGGGCTAATCAAACAGTAAATCAAGAAGATTTCTTGAGTTCAG
TCTGCAGAAGAGTTTTAAGTACAAATCTAAATGGACTTCATGAAACACAAGAAAATGAAATCTTAAATTTAGTGCAAATT
ACTGAGAAACAAATATCCACCTTCCCTGGTCCTATTCATTTAAATATTCCTATTGATAAGCCTTTAGGTATTTCTTTTTT
AAATAAAAAAAATGTTTTAGAGGTTTTTGAGAGAATTTATTTAAAGAAAAAATATGTCTTTCAGGAAGTTGAAATAAAGT
CTAATAAAAACAAATTCTTAGAAATTTTAGAAAATTTAAATTTAGATGAATCTGGCATTATTTTGGTAGGTCCCTATCAA
GGCTCGATAAATGAGTTATCTTCTTTCAATAAATCTTTAGAAAAATTACAAGCAATTACTGGTTGGCCAGTATTTGCTGA
TCCTGTTTCATGTGTTTATTCTGACTTGAGAGGGTTAGTTGTAAATTGGGAATTAGTCTTACGAAAAAATAAGAATTTAA
TCAATTGTCATCAACTTTTGAGACTTGGCCCTATGTCATCCTCAATTGATTTGGAGGATTTTTTAACAACCTTCGAAGGG
ATACAAATTCTTATTAAAGAAAAAAACTATCGAAAATTGGACCCTATAAAAAAATCATTTGAATATGATTTTGGACTATT
AAATTTTATTACTCTATTATTAGCAGAATTATCAATTAACAAAATAAACACAAAGTCTCTTACTCCGTTAGCCATTGATC
TAATGGAGGAAGGTGAGCAAATTAAAAAAATTTTAAAAGAGAAAATTACTAATGATAATCAAATTACTGAGTATAAGCTT
GCAAATCTTGTTCCAAAACTTTGGCCAGCTGAAAATCCAATAATGCTCTCCGCTAGTAGCCCAATTAGAGACTGGCTTAC
ATTTTCTGAGAATGGTACTTTAACAAGAAATTGTTTCAGCTTTAGAGGAGCTTCTGGAATCGACGGTACTTTATCACTTG
CATTAGGAATATCTAGAATAAAAAACCCTCTACTTCTTGTTACTGGAGATTTGGCTTTTGTTCATGATTTAAATGGCTGG
CTTATTGAAAATTCAATCGACTTAAATTTAACAATTTTATTAATAAATAATAATGGAGGTAATATATTTAACCGTCTTTA
TAAGGACAATCTAAAAGAAGATGAATTAAAAAAACTATTTCTTATGCCTAAAGAAATAAACTGGAAAAAACTCGCGGAAG
GCTATCAAGTAAACTTTAAAAGTGTGACAAATTTTAAAAAATTACGAGAGGCATTCGAATGGAGCATTTCTATTCGGGAA
TCTGTAATAATTAAAGTTGATATCGATCCGGAGAATGAAATTAGTGACAAAAATGCCCTGTTAGAAAAAATAATTGGCAG
TTAA

Upstream 100 bases:

>100_bases
TTTTTAAATCCTTTATTATTGAATTTCTTTTTTCTTCAGTTCTAGACATGTTTTATTTAATTCAATTATAAGGAAAAATC
CCAAAGCATTTATATAAATT

Downstream 100 bases:

>100_bases
TTTTTTTTATTTTCTATTTTTGAATAATTAGGTTTCATGAAAGTCTTACCTGGGAAAACTACTTTAAATTGGTCACAATA
TAAATCTTATGAGGATATAT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTSSIECKNFLRSLQLLNLLIKIGVQNLIVCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGNLSLV
ITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRRVLSTNLNGLHETQENEILNLVQI
TEKQISTFPGPIHLNIPIDKPLGISFLNKKNVLEVFERIYLKKKYVFQEVEIKSNKNKFLEILENLNLDESGIILVGPYQ
GSINELSSFNKSLEKLQAITGWPVFADPVSCVYSDLRGLVVNWELVLRKNKNLINCHQLLRLGPMSSSIDLEDFLTTFEG
IQILIKEKNYRKLDPIKKSFEYDFGLLNFITLLLAELSINKINTKSLTPLAIDLMEEGEQIKKILKEKITNDNQITEYKL
ANLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDLNGW
LIENSIDLNLTILLINNNGGNIFNRLYKDNLKEDELKKLFLMPKEINWKKLAEGYQVNFKSVTNFKKLREAFEWSISIRE
SVIIKVDIDPENEISDKNALLEKIIGS

Sequences:

>Translated_587_residues
MTSSIECKNFLRSLQLLNLLIKIGVQNLIVCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGNLSLV
ITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRRVLSTNLNGLHETQENEILNLVQI
TEKQISTFPGPIHLNIPIDKPLGISFLNKKNVLEVFERIYLKKKYVFQEVEIKSNKNKFLEILENLNLDESGIILVGPYQ
GSINELSSFNKSLEKLQAITGWPVFADPVSCVYSDLRGLVVNWELVLRKNKNLINCHQLLRLGPMSSSIDLEDFLTTFEG
IQILIKEKNYRKLDPIKKSFEYDFGLLNFITLLLAELSINKINTKSLTPLAIDLMEEGEQIKKILKEKITNDNQITEYKL
ANLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDLNGW
LIENSIDLNLTILLINNNGGNIFNRLYKDNLKEDELKKLFLMPKEINWKKLAEGYQVNFKSVTNFKKLREAFEWSISIRE
SVIIKVDIDPENEISDKNALLEKIIGS
>Mature_586_residues
TSSIECKNFLRSLQLLNLLIKIGVQNLIVCPGSRSAPLAIAAGELNKLGLVNIFNSIDERSAGFHSLGISAASGNLSLVI
TTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCGANQTVNQEDFLSSVCRRVLSTNLNGLHETQENEILNLVQIT
EKQISTFPGPIHLNIPIDKPLGISFLNKKNVLEVFERIYLKKKYVFQEVEIKSNKNKFLEILENLNLDESGIILVGPYQG
SINELSSFNKSLEKLQAITGWPVFADPVSCVYSDLRGLVVNWELVLRKNKNLINCHQLLRLGPMSSSIDLEDFLTTFEGI
QILIKEKNYRKLDPIKKSFEYDFGLLNFITLLLAELSINKINTKSLTPLAIDLMEEGEQIKKILKEKITNDNQITEYKLA
NLVPKLWPAENPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDLNGWL
IENSIDLNLTILLINNNGGNIFNRLYKDNLKEDELKKLFLMPKEINWKKLAEGYQVNFKSVTNFKKLREAFEWSISIRES
VIIKVDIDPENEISDKNALLEKIIGS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=600, Percent_Identity=25.5, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PROM9 (Q31BS7)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397104.1
- ProteinModelPortal:   Q31BS7
- STRING:   Q31BS7
- GeneID:   3765407
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0607
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK921906
- BioCyc:   PMAR74546:PMT9312_0607-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65734; Mature: 65603

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSIECKNFLRSLQLLNLLIKIGVQNLIVCPGSRSAPLAIAAGELNKLGLVNIFNSIDE
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
RSAGFHSLGISAASGNLSLVITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCG
HCCCCHHCCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRRVLSTNLNGLHETQENEILNLVQITEKQISTFPGPIHLNIPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PLGISFLNKKNVLEVFERIYLKKKYVFQEVEIKSNKNKFLEILENLNLDESGIILVGPYQ
CCCCEECCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCEEEEECCC
GSINELSSFNKSLEKLQAITGWPVFADPVSCVYSDLRGLVVNWELVLRKNKNLINCHQLL
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHH
RLGPMSSSIDLEDFLTTFEGIQILIKEKNYRKLDPIKKSFEYDFGLLNFITLLLAELSIN
HCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCC
KINTKSLTPLAIDLMEEGEQIKKILKEKITNDNQITEYKLANLVPKLWPAENPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDLNGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCE
LIENSIDLNLTILLINNNGGNIFNRLYKDNLKEDELKKLFLMPKEINWKKLAEGYQVNFK
EEECCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHCCCEEEHH
SVTNFKKLREAFEWSISIRESVIIKVDIDPENEISDKNALLEKIIGS
HHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TSSIECKNFLRSLQLLNLLIKIGVQNLIVCPGSRSAPLAIAAGELNKLGLVNIFNSIDE
CCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
RSAGFHSLGISAASGNLSLVITTSGTAVSNLLPAAVEADRSCKGIIFLTADRPLRLKDCG
HCCCCHHCCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRRVLSTNLNGLHETQENEILNLVQITEKQISTFPGPIHLNIPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PLGISFLNKKNVLEVFERIYLKKKYVFQEVEIKSNKNKFLEILENLNLDESGIILVGPYQ
CCCCEECCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCEEEEECCC
GSINELSSFNKSLEKLQAITGWPVFADPVSCVYSDLRGLVVNWELVLRKNKNLINCHQLL
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHH
RLGPMSSSIDLEDFLTTFEGIQILIKEKNYRKLDPIKKSFEYDFGLLNFITLLLAELSIN
HCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCC
KINTKSLTPLAIDLMEEGEQIKKILKEKITNDNQITEYKLANLVPKLWPAENPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDLNGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCE
LIENSIDLNLTILLINNNGGNIFNRLYKDNLKEDELKKLFLMPKEINWKKLAEGYQVNFK
EEECCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHCCCEEEHH
SVTNFKKLREAFEWSISIRESVIIKVDIDPENEISDKNALLEKIIGS
HHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA