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The map label for this gene is ilvB [H]

Identifier: 78778911

GI number: 78778911

Start: 492156

End: 493919

Strand: Direct

Name: ilvB [H]

Synonym: PMT9312_0526

Alternate gene names: 78778911

Gene position: 492156-493919 (Clockwise)

Preceding gene: 78778910

Following gene: 78778912

Centisome position: 28.79

GC content: 37.24

Gene sequence:

>1764_bases
GTGACTCTTACTTCGAGATCCTTGTTAAAGGATAGTTCAAAAAATGAAAATTCCGTTTGGATAACTGGTGCAGATGCACT
CATGGATGCTCTAAAAATTAATGGGGTAAAGGTTATATTTGGATATCCTGGAGGAGCTATATTACCAATATATGACTCTG
TTCATAAGGCAGAGCAAGATGGTTGGTTAAAGCATTATATGGTAAGACATGAACAAGGAGGTTCTCATGCGGCTGATGGA
TATGCGAGATCTACTGGTGAGGTAGGGGTATGTTTTGGGACCTCAGGTCCAGGTGCAACAAATTTGGTAACTGGAATTGC
AACTGCTCAAATGGATTCAATACCTCTAGTAGTAGTTACAGGTCAAGTTCCAAGACCTGCAATAGGGACAGATGCTTTTC
AAGAAACTGATATTTTTGGCATAACTCTTCCAATAGTTAAACATTCATGGGTAATAAGAGATCCTGCAAACATCGCGAAA
GTAGTTTCAGAAGCTTTTTTTATAGCCTCTTCTGGAAGACCCGGCCCTGTTTTAATTGACATACCCAAGGATGTAGGTCA
AGAATTCTTTCATTACCAAAGAGTTTTGCCTGGTGAGATTATTCCTAAGGGATTTAAAAGAAATGGAGACATTAATGATA
GTGATATCAAAAAGGCTATTAAATTAATAGAAGACTCTGAAAGACCTCTTCTCTATGTTGGCGGTGGTGCAATATCTTCC
GGGTCTCATGATGAAATAAGAACTTTGGCAAAAAATTATCAAATACCAGTTACTACGACATTAATGGGTAAAGGAGCTTT
TGACGAAAAAGATAATCTATCAGTTGGGATGTTAGGAATGCACGGAACTGCTTACGCAAACTTTGCAGTAACAGAATGTG
ATCTTTTAATAGCTATTGGAGCTAGATTTGATGATAGAGTGACTGGAAAATTAGATACTTTTGCACCTAATGCAAAGGTC
ATTCATATAGATATTGATCCAGCAGAAGTTAATAAAAATAGGCGTGTAGATGTTGCAATTGTCTCTGATGTTTCAAAAGC
TGTTCGCAAAATTAATGAAAAATCTCGGGATAACAAATTTGCTTGTCAGACGAAGAAATGGTTAGAAAAAATTGATTTTT
GGAAAAATAAACATCCCTTGTATGAACCTCCTCAAGAAGGAGAAATTTATCCTCAAGAAGTTCTTTTGAAAGTTAGGGAA
CTTTTACCTGAAGCTTATGTAACTACAGATGTAGGACAACATCAGATGTGGGCGGCTCAATATCTTAGGAATTCTCCAAG
AAAATGGATTAGTAGTGCTGGCTTAGGAACTATGGGTTTTGGATTGCCAGCGGCAATTGGAGTTAAAGCAGCCTTACCTA
ATTCAGATGTAATATGCATAGCTGGAGATGCTAGCGTCTTAATGAATATTCAAGAATTAGGAACCTTATCTCAATATGGT
TTAAATGTTAAGTTAATTATCATAAATAATCGCTGGCAAGGGATGGTGAGGCAATGGCAGGAAAGTTTCTACAATGAAAG
GTATTCCTCATCTGACATGAGTTGTGGCGAACCTGATTTTGTAAAACTTGCTGAGTCTTTCGGAGTTAAGGGATACTTAA
TTTCTGATAGAAAACAATTACAGAATGAATTACAACATGCGCTTAATCATGACGGCCCTGCCTTGATTAATATTCTTGTC
AGAAGAGGTGAAAATTGCTATCCAATGGTCCCTCCTGGGAAAAGTAACGCTCAAATGGTTGGATATGTTAATTGTGAAGA
ATAA

Upstream 100 bases:

>100_bases
ATTGAAATTTCTTTTGAATACTTTTCTGACATTACATTTCTAAATGAGGCAAAAGTATTTAATTGAGTTTAATATTTAAA
GTAAATATTGAATTTCTTTA

Downstream 100 bases:

>100_bases
TTTTTAAATAAGCCATTTTAAAAAATTTAATTTTAAAAAAAATCAAAATCTTAAAAATTTCTGAATTGAAAAATTTATCA
AAATTTATAATAATAATTTG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTLTSRSLLKDSSKNENSVWITGADALMDALKINGVKVIFGYPGGAILPIYDSVHKAEQDGWLKHYMVRHEQGGSHAADG
YARSTGEVGVCFGTSGPGATNLVTGIATAQMDSIPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPANIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFHYQRVLPGEIIPKGFKRNGDINDSDIKKAIKLIEDSERPLLYVGGGAISS
GSHDEIRTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKV
IHIDIDPAEVNKNRRVDVAIVSDVSKAVRKINEKSRDNKFACQTKKWLEKIDFWKNKHPLYEPPQEGEIYPQEVLLKVRE
LLPEAYVTTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
LNVKLIIINNRWQGMVRQWQESFYNERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKQLQNELQHALNHDGPALINILV
RRGENCYPMVPPGKSNAQMVGYVNCEE

Sequences:

>Translated_587_residues
MTLTSRSLLKDSSKNENSVWITGADALMDALKINGVKVIFGYPGGAILPIYDSVHKAEQDGWLKHYMVRHEQGGSHAADG
YARSTGEVGVCFGTSGPGATNLVTGIATAQMDSIPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPANIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFHYQRVLPGEIIPKGFKRNGDINDSDIKKAIKLIEDSERPLLYVGGGAISS
GSHDEIRTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKV
IHIDIDPAEVNKNRRVDVAIVSDVSKAVRKINEKSRDNKFACQTKKWLEKIDFWKNKHPLYEPPQEGEIYPQEVLLKVRE
LLPEAYVTTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
LNVKLIIINNRWQGMVRQWQESFYNERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKQLQNELQHALNHDGPALINILV
RRGENCYPMVPPGKSNAQMVGYVNCEE
>Mature_586_residues
TLTSRSLLKDSSKNENSVWITGADALMDALKINGVKVIFGYPGGAILPIYDSVHKAEQDGWLKHYMVRHEQGGSHAADGY
ARSTGEVGVCFGTSGPGATNLVTGIATAQMDSIPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPANIAKV
VSEAFFIASSGRPGPVLIDIPKDVGQEFFHYQRVLPGEIIPKGFKRNGDINDSDIKKAIKLIEDSERPLLYVGGGAISSG
SHDEIRTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKVI
HIDIDPAEVNKNRRVDVAIVSDVSKAVRKINEKSRDNKFACQTKKWLEKIDFWKNKHPLYEPPQEGEIYPQEVLLKVREL
LPEAYVTTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYGL
NVKLIIINNRWQGMVRQWQESFYNERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKQLQNELQHALNHDGPALINILVR
RGENCYPMVPPGKSNAQMVGYVNCEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=26.140350877193, Blast_Score=168, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=24.1962774957699, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=44.524236983842, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1790104, Length=575, Percent_Identity=42.2608695652174, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=31.7543859649123, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=26.4758497316637, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=24.5454545454545, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=590, Percent_Identity=25.9322033898305, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=596, Percent_Identity=25, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=603, Percent_Identity=25.0414593698176, Blast_Score=132, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=40.9317803660566, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=24.5901639344262, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=604, Percent_Identity=24.0066225165563, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=497, Percent_Identity=22.7364185110664, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=505, Percent_Identity=22.1782178217822, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.6637168141593, Blast_Score=177, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64406; Mature: 64275

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTSRSLLKDSSKNENSVWITGADALMDALKINGVKVIFGYPGGAILPIYDSVHKAEQD
CCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHHHC
GWLKHYMVRHEQGGSHAADGYARSTGEVGVCFGTSGPGATNLVTGIATAQMDSIPLVVVT
CHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPANIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCCEEEECHHHHHHHHHHHHEEECCCCCCCEEEE
IPKDVGQEFFHYQRVLPGEIIPKGFKRNGDINDSDIKKAIKLIEDSERPLLYVGGGAISS
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
GSHDEIRTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIG
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPNAKVIHIDIDPAEVNKNRRVDVAIVSDVSKAVRKINEKSRDNKF
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCC
ACQTKKWLEKIDFWKNKHPLYEPPQEGEIYPQEVLLKVRELLPEAYVTTDVGQHQMWAAQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHEECCCCCHHHHHHH
YLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
HHHCCHHHHHHHCCCCCCCCCCHHHHCEEEECCCCCEEEEECCHHHHHHHHHHCCHHHCC
LNVKLIIINNRWQGMVRQWQESFYNERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKQL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHH
QNELQHALNHDGPALINILVRRGENCYPMVPPGKSNAQMVGYVNCEE
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TLTSRSLLKDSSKNENSVWITGADALMDALKINGVKVIFGYPGGAILPIYDSVHKAEQD
CCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEECCCCEEEECHHHHHHHHHC
GWLKHYMVRHEQGGSHAADGYARSTGEVGVCFGTSGPGATNLVTGIATAQMDSIPLVVVT
CHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPANIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCCEEEECHHHHHHHHHHHHEEECCCCCCCEEEE
IPKDVGQEFFHYQRVLPGEIIPKGFKRNGDINDSDIKKAIKLIEDSERPLLYVGGGAISS
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
GSHDEIRTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIG
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPNAKVIHIDIDPAEVNKNRRVDVAIVSDVSKAVRKINEKSRDNKF
CCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCC
ACQTKKWLEKIDFWKNKHPLYEPPQEGEIYPQEVLLKVRELLPEAYVTTDVGQHQMWAAQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHEECCCCCHHHHHHH
YLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
HHHCCHHHHHHHCCCCCCCCCCHHHHCEEEECCCCCEEEEECCHHHHHHHHHHCCHHHCC
LNVKLIIINNRWQGMVRQWQESFYNERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKQL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHH
QNELQHALNHDGPALINILVRRGENCYPMVPPGKSNAQMVGYVNCEE
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]