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The map label for this gene is xthA [H]

Identifier: 78778870

GI number: 78778870

Start: 453971

End: 454816

Strand: Reverse

Name: xthA [H]

Synonym: PMT9312_0485

Alternate gene names: 78778870

Gene position: 454816-453971 (Counterclockwise)

Preceding gene: 78778875

Following gene: 78778869

Centisome position: 26.61

GC content: 31.44

Gene sequence:

>846_bases
TTGTTAATAGCAACTTGGAATGTTAACTCTATAAGAACCAGACTTTCACAAATAATAGATTGGATGAATCAAGTCAATCC
AGATATTCTATGTTTGCAGGAAACAAAAGTAATGGATAATAGTTTCCCATTTGAACCTTTTGAAAAATTAGGATACTCAG
TGGAGGTCTACGGACAAAAAGCATATAATGGTGTTGCTATTATTTCTAAAATAAAAGCAGAAAATGTTAAAAAAGGATTC
TACGGTTGTTCAGACTCTGATCAAAATGCCGAAATTTTCCTTGATCAAAAAAGATTAATTTCTGCTGATATTAATGGTAT
CAAGATTATAAATGTCTATGTGCCAAATGGATCTTCTCTAGAATCTAATAAGTTCGAATACAAAATTAATTGGTTAAGTA
GTTTAGCTTCATATTTGGATGAGCAAGAAAAAAAAGGAGAATTAATTTGTCTTATGGGTGATTTTAATGTTGCTCCCTCT
AACTTGGATATTTATGATCCCAAGAAATATGAAGGAGGAATAATGGCATCTGAGATAGAGAGAAATGCACTAAATAATGT
TCTGAAAGAAAGATTAATAGATTCTTTTAGGATTTTTGAAAAAAATCCTGGCCATTGGAGTTGGTGGGATTACCGTAATA
ACGCTTATGAATTAAATAAAGGTTGGAGGATAGACCATATCTACATCAGCAAAGAACTGTCATCAAAACTTAAAAGTTGT
GTCATAGACAGCTCACCAAGAGCGAATTTACGTCCAAGTGATCATGCACCAGTAATGATTGATCTTAACTTGAACGATAT
AAATGTAGATTTTTTTGAGGAAGAGGATAATTTTTTCGAAATATAA

Upstream 100 bases:

>100_bases
GGAGGGTTTTTGCAAAAATAATTTTTCATTTTATTTAATTTCAAAATTCTTTAACGCCGAAAATTCCTGCTAACTTAATT
CTACATAATTTGGTAGAAAT

Downstream 100 bases:

>100_bases
ATAAAATAAATTGAATTTTTTTAATATCTGAAAACGCTTATTAATAAAGAGATATCTAGCATGATCTTGATTTCTATCAT
GATTTCCTAAAAATAAATAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLIATWNVNSIRTRLSQIIDWMNQVNPDILCLQETKVMDNSFPFEPFEKLGYSVEVYGQKAYNGVAIISKIKAENVKKGF
YGCSDSDQNAEIFLDQKRLISADINGIKIINVYVPNGSSLESNKFEYKINWLSSLASYLDEQEKKGELICLMGDFNVAPS
NLDIYDPKKYEGGIMASEIERNALNNVLKERLIDSFRIFEKNPGHWSWWDYRNNAYELNKGWRIDHIYISKELSSKLKSC
VIDSSPRANLRPSDHAPVMIDLNLNDINVDFFEEEDNFFEI

Sequences:

>Translated_281_residues
MLIATWNVNSIRTRLSQIIDWMNQVNPDILCLQETKVMDNSFPFEPFEKLGYSVEVYGQKAYNGVAIISKIKAENVKKGF
YGCSDSDQNAEIFLDQKRLISADINGIKIINVYVPNGSSLESNKFEYKINWLSSLASYLDEQEKKGELICLMGDFNVAPS
NLDIYDPKKYEGGIMASEIERNALNNVLKERLIDSFRIFEKNPGHWSWWDYRNNAYELNKGWRIDHIYISKELSSKLKSC
VIDSSPRANLRPSDHAPVMIDLNLNDINVDFFEEEDNFFEI
>Mature_281_residues
MLIATWNVNSIRTRLSQIIDWMNQVNPDILCLQETKVMDNSFPFEPFEKLGYSVEVYGQKAYNGVAIISKIKAENVKKGF
YGCSDSDQNAEIFLDQKRLISADINGIKIINVYVPNGSSLESNKFEYKINWLSSLASYLDEQEKKGELICLMGDFNVAPS
NLDIYDPKKYEGGIMASEIERNALNNVLKERLIDSFRIFEKNPGHWSWWDYRNNAYELNKGWRIDHIYISKELSSKLKSC
VIDSSPRANLRPSDHAPVMIDLNLNDINVDFFEEEDNFFEI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=272, Percent_Identity=31.25, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI18375503, Length=272, Percent_Identity=31.25, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI18375501, Length=272, Percent_Identity=31.25, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=34.5588235294118, Blast_Score=147, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=27.9245283018868, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=31.8681318681319, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=31.8681318681319, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32454; Mature: 32454

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATWNVNSIRTRLSQIIDWMNQVNPDILCLQETKVMDNSFPFEPFEKLGYSVEVYGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCEEEEECCC
AYNGVAIISKIKAENVKKGFYGCSDSDQNAEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECHHEEECCCCCEEEEEEEECCCCCC
ESNKFEYKINWLSSLASYLDEQEKKGELICLMGDFNVAPSNLDIYDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RNALNNVLKERLIDSFRIFEKNPGHWSWWDYRNNAYELNKGWRIDHIYISKELSSKLKSC
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCEEEEEEEECHHHHHHHHHH
VIDSSPRANLRPSDHAPVMIDLNLNDINVDFFEEEDNFFEI
CCCCCCCCCCCCCCCCCEEEEEECCEEEEEEEECCCCEECC
>Mature Secondary Structure
MLIATWNVNSIRTRLSQIIDWMNQVNPDILCLQETKVMDNSFPFEPFEKLGYSVEVYGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCEEEEECCC
AYNGVAIISKIKAENVKKGFYGCSDSDQNAEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECHHEEECCCCCEEEEEEEECCCCCC
ESNKFEYKINWLSSLASYLDEQEKKGELICLMGDFNVAPSNLDIYDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RNALNNVLKERLIDSFRIFEKNPGHWSWWDYRNNAYELNKGWRIDHIYISKELSSKLKSC
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCEEEEEEEECHHHHHHHHHH
VIDSSPRANLRPSDHAPVMIDLNLNDINVDFFEEEDNFFEI
CCCCCCCCCCCCCCCCCEEEEEECCEEEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]