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The map label for this gene is gltX

Identifier: 78778858

GI number: 78778858

Start: 444370

End: 445809

Strand: Reverse

Name: gltX

Synonym: PMT9312_0473

Alternate gene names: 78778858

Gene position: 445809-444370 (Counterclockwise)

Preceding gene: 78778859

Following gene: 78778854

Centisome position: 26.08

GC content: 32.78

Gene sequence:

>1440_bases
TTGGAAATACGTTTAAGATTAGCGCCGAGTCCAACAGGTTTATTTCATATTGGAACAGCTCGAACAGCGTTATTCAACTG
GTTGTATGCACAAAAAATAGGTGGAAAATTCCTCATAAGAATTGAAGATACAGATTTTCTTCGATCTAAATCTGAATACA
CAAAAAATATATTAGATGGCTTGAAATGGCTTGGACTTCAATGGAATGAAGAACCTGTAAAGCAAAGTGACAGAATTTCG
ATTCACAAAAAATACATCAAAAAGCTATTAGAATGTGGAGCTGCATATAGATGCTTCACTACAGAAGATGAGATTTCTGA
ATTAAGAGAGGAACAAAAAAAGAAAGGATTACCTCCAAAGCATGATAATAGGCATAGAAATCTTTCTAAAGAAGAAATAG
AAACATTCATATCCCAAGGAAGGACTTCCGTAATAAGGTTTAAGATTGATGAGGAAATACAAATTAAATGGATAGATCAG
ATAAGAGGCGAAATTAAATGGCAAGGTAAGGACTTAGGTGGTGATTTGGTTTTATCAAGAAGGGCTATGGGGTATGAGAT
TGGAGATCCTTTGTATAATCTTGCAGTTGTAGTTGATGATAATTTCATGAATATCACTCATGTCGTAAGGGGTGAAGACC
ATATCTCTAATACGGCAAAACAAATCTTGATTTATGAAGCTTTAGATTTCAAACTACCAACTTTTTCACACACACCGCTA
ATACTAAATAACGAAGGGAAGAAATTATCCAAGAGAGATTGCGTTACTTCAATCGACGAATTTAGAGATATGGGATATTT
ACCGGAGGCCTTATCGAACTATATGGCCTTTCTAGGTTGGTCGCCAAAATCTGCCACGAGTGAAATACTTTCACTTGATG
AGATATCTAAAATTTTTGAATTATCAGATATAAATAAAGCTGGAGCTAAATTCAGTTGGGAAAAACTCAATTGGATTAAT
TCTCAATACATAAAAAATATGGAAACAGTAAAATTGAGTGAGACCATAGGAAAATACTGGGATAATATGGGTTGGGATCC
ACCATCTCAAGAATGGGCTATTAAATTAGCAATTTTGATTAAAGACTCGATGACTCTTTTAAAAGACGCCATTGATCAAT
CAAAACCATTTTTCTTATTACCCCCAATTCAAAAAGAAGGTAAAGATTTTCTTGAAAGCAATGATGGCAAAACATCTCTA
AAACTAATTTTAAATTATTTAATTGAGCAAAATACTGGAAAAGTAGATAAAGATAAAGCCAAAGAAATAATAAACGAAAT
CTCAAAAATGCATAATGTTAAAAAAGGTATTTTAATGAAATCATTAAGAGTAGCCTTTTTTGGTTCTCTCAGTGGGCCAG
ATTTAATTCAAAGTTGGGAACTTTTCTCTGAGAGTAAAAGTGATATATCAAGAATTGAAAGATGTCTTAAATCAATCTAA

Upstream 100 bases:

>100_bases
TAAAACTCTGGGACCGTAGTTCAACTGGTTAGAGCACCGCCCTGTCACGGCGGAAGTTGCGGGTTCGAATCCCGTCGGTC
CCGTTTTTTCTAAAAAAAAT

Downstream 100 bases:

>100_bases
TTTTTTTTTTGGCCTAATTCAAGCCGATTTACCAAAGGTTTAGCTGTAAGGCCTTGGATTCCTACCGTCATTAATATAGT
AAGAAACACTAAACCTTGAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MEIRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILDGLKWLGLQWNEEPVKQSDRIS
IHKKYIKKLLECGAAYRCFTTEDEISELREEQKKKGLPPKHDNRHRNLSKEEIETFISQGRTSVIRFKIDEEIQIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAMGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYEALDFKLPTFSHTPL
ILNNEGKKLSKRDCVTSIDEFRDMGYLPEALSNYMAFLGWSPKSATSEILSLDEISKIFELSDINKAGAKFSWEKLNWIN
SQYIKNMETVKLSETIGKYWDNMGWDPPSQEWAIKLAILIKDSMTLLKDAIDQSKPFFLLPPIQKEGKDFLESNDGKTSL
KLILNYLIEQNTGKVDKDKAKEIINEISKMHNVKKGILMKSLRVAFFGSLSGPDLIQSWELFSESKSDISRIERCLKSI

Sequences:

>Translated_479_residues
MEIRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILDGLKWLGLQWNEEPVKQSDRIS
IHKKYIKKLLECGAAYRCFTTEDEISELREEQKKKGLPPKHDNRHRNLSKEEIETFISQGRTSVIRFKIDEEIQIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAMGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYEALDFKLPTFSHTPL
ILNNEGKKLSKRDCVTSIDEFRDMGYLPEALSNYMAFLGWSPKSATSEILSLDEISKIFELSDINKAGAKFSWEKLNWIN
SQYIKNMETVKLSETIGKYWDNMGWDPPSQEWAIKLAILIKDSMTLLKDAIDQSKPFFLLPPIQKEGKDFLESNDGKTSL
KLILNYLIEQNTGKVDKDKAKEIINEISKMHNVKKGILMKSLRVAFFGSLSGPDLIQSWELFSESKSDISRIERCLKSI
>Mature_479_residues
MEIRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILDGLKWLGLQWNEEPVKQSDRIS
IHKKYIKKLLECGAAYRCFTTEDEISELREEQKKKGLPPKHDNRHRNLSKEEIETFISQGRTSVIRFKIDEEIQIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAMGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYEALDFKLPTFSHTPL
ILNNEGKKLSKRDCVTSIDEFRDMGYLPEALSNYMAFLGWSPKSATSEILSLDEISKIFELSDINKAGAKFSWEKLNWIN
SQYIKNMETVKLSETIGKYWDNMGWDPPSQEWAIKLAILIKDSMTLLKDAIDQSKPFFLLPPIQKEGKDFLESNDGKTSL
KLILNYLIEQNTGKVDKDKAKEIINEISKMHNVKKGILMKSLRVAFFGSLSGPDLIQSWELFSESKSDISRIERCLKSI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=481, Percent_Identity=29.3139293139293, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1788743, Length=487, Percent_Identity=34.9075975359343, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=33.201581027668, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=25.2293577981651, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=473, Percent_Identity=30.4439746300211, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=466, Percent_Identity=31.7596566523605, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=31.58953722334, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PROM9 (Q31C61)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396970.1
- ProteinModelPortal:   Q31C61
- SMR:   Q31C61
- STRING:   Q31C61
- GeneID:   3765270
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0473
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   DAQAREW
- ProtClustDB:   PRK01406
- BioCyc:   PMAR74546:PMT9312_0473-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55167; Mature: 55167

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILDG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLQWNEEPVKQSDRISIHKKYIKKLLECGAAYRCFTTEDEISELREEQKKKGLPPK
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCC
HDNRHRNLSKEEIETFISQGRTSVIRFKIDEEIQIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHCCEEEECCCCCCCHHEEHH
RAMGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYEALDFKLPTFSHTPL
HHCCCCCCCCCEEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNNEGKKLSKRDCVTSIDEFRDMGYLPEALSNYMAFLGWSPKSATSEILSLDEISKIFE
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LSDINKAGAKFSWEKLNWINSQYIKNMETVKLSETIGKYWDNMGWDPPSQEWAIKLAILI
HHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEE
KDSMTLLKDAIDQSKPFFLLPPIQKEGKDFLESNDGKTSLKLILNYLIEQNTGKVDKDKA
HHHHHHHHHHHHCCCCEEEECCCCHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHH
KEIINEISKMHNVKKGILMKSLRVAFFGSLSGPDLIQSWELFSESKSDISRIERCLKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILDG
CEEEEEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLQWNEEPVKQSDRISIHKKYIKKLLECGAAYRCFTTEDEISELREEQKKKGLPPK
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCC
HDNRHRNLSKEEIETFISQGRTSVIRFKIDEEIQIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEEHHHHCCEEEECCCCCCCHHEEHH
RAMGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYEALDFKLPTFSHTPL
HHCCCCCCCCCEEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCE
ILNNEGKKLSKRDCVTSIDEFRDMGYLPEALSNYMAFLGWSPKSATSEILSLDEISKIFE
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LSDINKAGAKFSWEKLNWINSQYIKNMETVKLSETIGKYWDNMGWDPPSQEWAIKLAILI
HHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEE
KDSMTLLKDAIDQSKPFFLLPPIQKEGKDFLESNDGKTSLKLILNYLIEQNTGKVDKDKA
HHHHHHHHHHHHCCCCEEEECCCCHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHH
KEIINEISKMHNVKKGILMKSLRVAFFGSLSGPDLIQSWELFSESKSDISRIERCLKSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA