Click here to switch to the map view.

The map label for this gene is ndhB [H]

Identifier: 78778819

GI number: 78778819

Start: 408652

End: 410172

Strand: Reverse

Name: ndhB [H]

Synonym: PMT9312_0434

Alternate gene names: 78778819

Gene position: 410172-408652 (Counterclockwise)

Preceding gene: 78778825

Following gene: 78778818

Centisome position: 24.0

GC content: 35.77

Gene sequence:

>1521_bases
GTGCCCAACGAAATCTTTACAATTAACTTAAATGCTCAAGCCATTATTCCAGAGGCTTTTATCTTACTAGGTATTGTTGG
GACTCTTCTTGTAGATTTAGCAGGAGAAAAAACTGCATCAAAATGGGCACCAATAATTTGCTATTTATCAATTGGTAGTT
CACTTTTTAGTTTAGCCTTGCAATGGAGTAATCCAGTAAATAGTGCATTTCTTGGTTCCTTTAATTCAGATAATTTGGCA
ATCGCATTTAGAGCAATAATAGCTTTATCAACTTTAGTTTCTTTACTTATAAGCTGGCGTTATACAGAACAAAGTGGAAG
CCCAATTGGGGAGTTCGCAGCTATAGTTCTTTCGGCAACTCTTGGAGCAATGCTTTTGTGTGGATCTACTGACCTTATTA
GTATATTTATATCTCTTGAGACTTTGTCGGTAGCAAGCTATTTACTTTCTGGTTACCTCAAGAGAGATCCAAGAAGTTCA
GAGGCCGCTTTAAAATATCTACTTGTTGGTTCAGCTGCTGCTGCTGTCTATTTGTATGGCTCCTCTTTTCTTTATGGATT
AAGTGGTTCAACAAACTTAGCAACAATTGGTTTAGAGATAATCAACAAGCCATCATTCATTACTTCTCTAGCTCTTGTAT
TTGTTTTATCAACTGTTGCATTTAAAATTGCTGCAGTACCCTTTCATCAATGGACTCCTGATGTATATGAGGGTTCACCT
ACACCTGTAGTAGCTTTTTTATCTGTTGGCTCAAAAACAGCAGGATTTGCATTTGCGATAAGAATATTAAGCACTACTTT
CTCTTCTTTCGATGAAGAATGGAAACTTTTATTTACTATTTTGGCCATCTTGAGTATGGCTCTAGGAAATGTTGTAGCCC
TTGCTCAAACCTCAATGAAAAGGATGTTGGCTTACAGTTCTATTGGTCAAGCGGGATTTGTAATGATTGGAATAGTATCT
GGAACACAAGATGGTTTATCAGCTACTGTTTTATACTTAGCTGCATATTTATTTATGAATTTGGGTGCATTTTCATGTGT
AATACTTTTCTCATTAAGAACTGGATCTGACAGAATTCTCGATTACTCAGGACTTTACCAAAAGGATCCTCTCATTACAT
TAGGCTTAAGCCTTTGTCTTCTTTCTCTTGGAGGTTTACCTCCAATGTTAGGATTTTTTGGAAAGATATATTTGTTCTTT
GCAGGTTGGGCTAATCATCAATATTTATTAGTAATCATTGGATTAGTAACTTCAGTCATATCTATTTATTACTACATTTC
GGTTATAAAAATGATGGTAGTTAAAGAACCACAAGAAGCTTCTGAAATAGTTAAATCATATCCTGAGATTAATTGGGGGA
TTGTAGGAATGCCCCCTTTGAGAGTTGCACTTTATACCTGCGTGGCGGTAACAGCTCTTGGAGGAATTCTATCTAACCCT
CTTTTTAAATTAGCTAACACAGCAGTTTCAGAAACTCCTTTTTTACAAGAAATTATTGCTATGGCAAACAATATTTCCTA
G

Upstream 100 bases:

>100_bases
TCCTGAAAATTTCAAAAATCATACCCACAAATTATAATCAAGTTAAGATTAACTCTTTGACCCTTACAATCAAACATCTA
ATTTAATCCAATTTAATAAA

Downstream 100 bases:

>100_bases
AAGAATTCCTCAATAAAAAATGTCTAAGAAAGTCGCAAGTTTAGAGAGAATATCTAAAAAATATGGGAAAGATGATCTAA
CTGTTAAAGCCTTAGACAGC

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2 [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLFSLALQWSNPVNSAFLGSFNSDNLA
IAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISIFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSATVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIIGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIVGMPPLRVALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQEIIAMANNIS

Sequences:

>Translated_506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLFSLALQWSNPVNSAFLGSFNSDNLA
IAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISIFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSATVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIIGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIVGMPPLRVALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQEIIAMANNIS
>Mature_505_residues
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLFSLALQWSNPVNSAFLGSFNSDNLAI
AFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISIFISLETLSVASYLLSGYLKRDPRSSE
AALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSPT
PVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVSG
TQDGLSATVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFA
GWANHQYLLVIIGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIVGMPPLRVALYTCVAVTALGGILSNPL
FKLANTAVSETPFLQEIIAMANNIS

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=409, Percent_Identity=23.960880195599, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693160, Length=386, Percent_Identity=33.419689119171, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788829, Length=456, Percent_Identity=24.1228070175439, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=27.6094276094276, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788614, Length=431, Percent_Identity=24.5939675174014, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788613, Length=360, Percent_Identity=24.1666666666667, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54181; Mature: 54050

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLFSLAL
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
QWSNPVNSAFLGSFNSDNLAIAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSAT
HCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISIFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSATVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIIGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIVGMPPLRVALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQEIIAMANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLFSLAL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
QWSNPVNSAFLGSFNSDNLAIAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSAT
HCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISIFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSATVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIIGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIVGMPPLRVALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQEIIAMANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA