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The map label for this gene is ndhK [H]

Identifier: 78778680

GI number: 78778680

Start: 283490

End: 284224

Strand: Reverse

Name: ndhK [H]

Synonym: PMT9312_0295

Alternate gene names: 78778680

Gene position: 284224-283490 (Counterclockwise)

Preceding gene: 78778681

Following gene: 78778679

Centisome position: 16.63

GC content: 36.19

Gene sequence:

>735_bases
TTGAACCCACAATTATCCCCAAAAGCTATAAGGGAAATCAGAGAAGGAACTTGCAATCCCCTTGGTGCACCACAAGTAAC
TACAGATTTAAGTGAAAATATAATCCTAACAAGCTTAGATGATCTTCATAATTGGGCTAGATTAAGTAGTCTTTGGCCTC
TTCTATACGGAACAGCTTGTTGTTTTATTGAATTTGCTGCACTAATTGGTTCTAGATTTGATTTTGACAGGTTTGGATTA
GTACCGAGAAGCTCTCCAAGACAAGCAGATCTATTAATAGTCGCAGGGACAGTAACGATGAAAATGGCTCCAGCCCTAGT
AAGACTTTATGAGCAGATGCCTGAGCCAAAATATGTTATTGCAATGGGTGCTTGTACAATCACAGGTGGAATGTTTAGCG
CAGATTCTACTACTGCTGTCAGAGGCGTTGATAAATTAATACCAGTCGATTTATATCTTCCAGGATGTCCACCAAGACCA
GAAGCAATTTTTGATGCTGTAATAAAATTAAGAAAAAAAGTAGGTAATGAATCAATTTTAGAGCGGGAAAAAACAGAGCA
AACCCACAGGTACATAACATATAAACACGAAATGAATCTTGTTTTCTCTCAAAATACTGGTGAATATTTAAACAAAACTT
CAACGAATGTTTTTCCTTCCTCCAAAAAAGAAAAAATAACTGAATTACCTGAAAACAGGGAACAAACCGAAATTATTAAT
AGTGAAGAAGAGTAA

Upstream 100 bases:

>100_bases
GGCTTATTAGCATTTATTGAGGCACTGATTTTCATTGCAATACTCGTTATCGCTTTGGCTTACGCATGGAGAAAAGGTGC
TTTAGAATGGAGTTAAAAAA

Downstream 100 bases:

>100_bases
TGGAAAAAGACGGTTTAGCTAAATCCACAGATACTTCAATAGAAAAAGAAGGATTCATGAGTCAAAATTTATCTAAAGTT
GGCATTCCAAACCAACCGTT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILEREKTEQTHRYITYKHEMNLVFSQNTGEYLNKTSTNVFPSSKKEKITELPENREQTEIIN
SEEE

Sequences:

>Translated_244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILEREKTEQTHRYITYKHEMNLVFSQNTGEYLNKTSTNVFPSSKKEKITELPENREQTEIIN
SEEE
>Mature_244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILEREKTEQTHRYITYKHEMNLVFSQNTGEYLNKTSTNVFPSSKKEKITELPENREQTEIIN
SEEE

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=51.7006802721088, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=48.1707317073171, Blast_Score=170, Evalue=6e-44,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=49.6732026143791, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651058, Length=143, Percent_Identity=53.1468531468531, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 27343; Mature: 27343

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
EREKTEQTHRYITYKHEMNLVFSQNTGEYLNKTSTNVFPSSKKEKITELPENREQTEIIN
HHHHHHHHHHEEEEEEEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHCC
SEEE
CCCC
>Mature Secondary Structure
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
EREKTEQTHRYITYKHEMNLVFSQNTGEYLNKTSTNVFPSSKKEKITELPENREQTEIIN
HHHHHHHHHHEEEEEEEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHCC
SEEE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA