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The map label for this gene is 78778667

Identifier: 78778667

GI number: 78778667

Start: 270054

End: 271538

Strand: Reverse

Name: 78778667

Synonym: PMT9312_0282

Alternate gene names: NA

Gene position: 271538-270054 (Counterclockwise)

Preceding gene: 78778668

Following gene: 78778664

Centisome position: 15.89

GC content: 33.33

Gene sequence:

>1485_bases
GTGACTAATTTACAAGATAAAAAAATTGATAAATTTAAAAGTTTTAATAAAGAAGATTGGTCAAGTGCGTATCAAAATGT
AGAAAAGGAGCTAACCAAGGAGCCTCTAAAAATTAGCAAAGGTAATAATATAAAAAATTTAAATGGAACATTATTAAGAA
ATGGACCAGGAATATTAGAGAGAGGTGGACAATGGGTTCATCATCCATTTGATGGTGATGGAATGATAACTTCCATAAAA
TTCGAAAATGGTCAGCCATTTTTAACAAATAGATTTGTTAAAACTAAAGGCTATTTGGAAGAAGAAAAAATAAATAAATT
TATTTATAGAGGTGTATTTGGGACACAAAAAAATGGAGGAATTCTAAATAATGCATTAGATCTAAAATTCAAGAATATCG
CTAATACTCATGTTGTTAAATTAGGAGATGAAATTCTCGCATTATGGGAAGCAGCCGGTCCACATGCAATGGATCCTGAT
AGTCTTAACACTATTGGTTTAACAACATTAAAAGGGGTACTCAAGCCTAACGAAGCATTTAGTGCCCATCCCAAAACAGA
CCTAAATTCAAATGCATCTTCAGAACTTTTAGTCACTTTTGGAGTACAAACCGGGCCAAAAAGTACCATTAGATTAATGG
AATTTAATAATGCTGGTACAAATTCTGGAGAGCTCATTTTCGACAGAAAAGATACCCTTAATGGCTTTGCATTCCTTCAT
GATTTCGCAATTACAACTAATTGGGCAATATTTTTACAGAATGCTATTGATTTCAATCCTCTTCCATTTGTAATGGGCCA
AAGAGGAGCAGCTCAATGTCTAAAGTCAAACCCAAATAAAAAGGCAAAGTTTTTTATCATCCCCAGAGAAAGTGGATTAT
TTAGAGGTCAGCCACCGCTAACAATAGATGCTCCAGAAGGATTCGTTTTTCATCATGTAAATGCATTTGAAAAAGATTCC
AAAATCATATTAGATAGTATTTTTTATGATGATTTTCCATCAGTTGGTCCAGACGAGAATTTTAGGGATATTGACTTTGA
TAAATATCCAGAAGGAAAACTGAAAAGATCAATTATCGATCTAAAGAAAAAAACTAGTGAACTTGAAACTTTAAGTGAAC
AATGTTGTGAATTTGCTGTTGTTAATCCTAAAAACTTAGGATTAACAGCAACTTTTAGTTGGATGGCAAGCACATCTCAA
AAGATGGGGAACGCTCCACTGCAAGCAATAAAAAAAATAAATTTAACATCTAAGGAAGAGATTTCTTGGTCAGCTGGTCC
AAGTGGATTCGTAAGTGAACCAATCATGGTTCCATCAGAAAACTCCTCTAAGGAAGATGAGGGATTTTTGTTTATACTTT
TATGGAACGGAGAAAGAAGAGGAAGCGATTTAGTGATATTAGATGCAAAAGACTTAAAAGAATTAGCTGTTTATGAATTA
CCCATTTCAATTCCTCATGGCCTTCATGGATCTTGGGTTAATTGA

Upstream 100 bases:

>100_bases
CAGTAAAGGAATGCTAGAAAATCAATAAAATAGCAATTTTTTCGAATCAGCCACAATTCAGTTGATTTTTGGCGAAAATA
AAAGGAGTAATTATTTTGTT

Downstream 100 bases:

>100_bases
ATTTAAGAAAAAATTTCAATTAAATCTGGAATAATTTTTTTTAATGCTTTACCTCTATGACTACAAGAGTCTTTAATATC
ATTATTCATTTCTGCAAAAG

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTNLQDKKIDKFKSFNKEDWSSAYQNVEKELTKEPLKISKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIK
FENGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVVKLGDEILALWEAAGPHAMDPD
SLNTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFNNAGTNSGELIFDRKDTLNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDS
KIILDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKKKTSELETLSEQCCEFAVVNPKNLGLTATFSWMASTSQ
KMGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
PISIPHGLHGSWVN

Sequences:

>Translated_494_residues
MTNLQDKKIDKFKSFNKEDWSSAYQNVEKELTKEPLKISKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIK
FENGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVVKLGDEILALWEAAGPHAMDPD
SLNTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFNNAGTNSGELIFDRKDTLNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDS
KIILDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKKKTSELETLSEQCCEFAVVNPKNLGLTATFSWMASTSQ
KMGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
PISIPHGLHGSWVN
>Mature_493_residues
TNLQDKKIDKFKSFNKEDWSSAYQNVEKELTKEPLKISKGNNIKNLNGTLLRNGPGILERGGQWVHHPFDGDGMITSIKF
ENGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGGILNNALDLKFKNIANTHVVKLGDEILALWEAAGPHAMDPDS
LNTIGLTTLKGVLKPNEAFSAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFNNAGTNSGELIFDRKDTLNGFAFLHD
FAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPLTIDAPEGFVFHHVNAFEKDSK
IILDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIIDLKKKTSELETLSEQCCEFAVVNPKNLGLTATFSWMASTSQK
MGNAPLQAIKKINLTSKEEISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYELP
ISIPHGLHGSWVN

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71998242, Length=516, Percent_Identity=24.031007751938, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32564756, Length=546, Percent_Identity=23.992673992674, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55081; Mature: 54950

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLQDKKIDKFKSFNKEDWSSAYQNVEKELTKEPLKISKGNNIKNLNGTLLRNGPGILE
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCHHH
RGGQWVHHPFDGDGMITSIKFENGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGG
CCCCEEECCCCCCCEEEEEEECCCCCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
ILNNALDLKFKNIANTHVVKLGDEILALWEAAGPHAMDPDSLNTIGLTTLKGVLKPNEAF
EECCEEEEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCCCC
SAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFNNAGTNSGELIFDRKDTLNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHEE
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPL
HHHHHCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSKIILDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIID
EEECCCCEEEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LKKKTSELETLSEQCCEFAVVNPKNLGLTATFSWMASTSQKMGNAPLQAIKKINLTSKEE
HHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHCCCCCCCC
ISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
CCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHEEEEC
PISIPHGLHGSWVN
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TNLQDKKIDKFKSFNKEDWSSAYQNVEKELTKEPLKISKGNNIKNLNGTLLRNGPGILE
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCHHH
RGGQWVHHPFDGDGMITSIKFENGQPFLTNRFVKTKGYLEEEKINKFIYRGVFGTQKNGG
CCCCEEECCCCCCCEEEEEEECCCCCCHHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
ILNNALDLKFKNIANTHVVKLGDEILALWEAAGPHAMDPDSLNTIGLTTLKGVLKPNEAF
EECCEEEEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCCCC
SAHPKTDLNSNASSELLVTFGVQTGPKSTIRLMEFNNAGTNSGELIFDRKDTLNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHEE
DFAITTNWAIFLQNAIDFNPLPFVMGQRGAAQCLKSNPNKKAKFFIIPRESGLFRGQPPL
HHHHHCCEEEEEECCCCCCCCCHHHCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSKIILDSIFYDDFPSVGPDENFRDIDFDKYPEGKLKRSIID
EEECCCCEEEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LKKKTSELETLSEQCCEFAVVNPKNLGLTATFSWMASTSQKMGNAPLQAIKKINLTSKEE
HHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHCCCCCCCC
ISWSAGPSGFVSEPIMVPSENSSKEDEGFLFILLWNGERRGSDLVILDAKDLKELAVYEL
CCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHEEEEC
PISIPHGLHGSWVN
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]