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The map label for this gene is pyrB

Identifier: 78778621

GI number: 78778621

Start: 226950

End: 227969

Strand: Reverse

Name: pyrB

Synonym: PMT9312_0235

Alternate gene names: 78778621

Gene position: 227969-226950 (Counterclockwise)

Preceding gene: 78778623

Following gene: 78778618

Centisome position: 13.34

GC content: 32.94

Gene sequence:

>1020_bases
ATGCAAACAATTTGGCCTCATAAACATATCCACACACTCGCTAATTTTTCAATTCAAGATTACAAATCAGTATTTGAATT
GGCTCATAGATTTGATGTACTAAAGAATGCAGGAACGAAAAAAATACCGGCTTTACAGGGGACTTTGGTAACGTCTTTGT
TTTTTGAAGCTAGTACAAGAACAAAAAATAGCTTTGAACTTGCAGCAAAAAGGCTCTCTGCCGATGTACAAACGTTTGCA
CCATCCTCTAGTTCTTTAACTAAAGGCGAAACAATAATTGATACTGCCTTAACTTATTCTGCTATGGGAGCGGATACATT
AGTTATTAGACATTCATCGAGTTACATAACTTTTGAGATAGCTAAAAAACTTGATGCAATAAATGCGAAGACTTCGGTTC
TTAATGCGGGAGATGGATTACATAGTCATCCTAGCCAAGGGTTGCTTGACATTTATACATTAATAAAATTCTTTTCCAAA
AAGTCACTGAGTCCAGATGTTTTAAATTCCAAAAAAATTTTAATAATTGGAGATGTTAATCATTCAAGGGTTGCAAGATC
AAATCTTTGGGCTTTGAGTGCATTCGGCGCAGACATCATCTTATGTGGTCCTAAAACATTAATACCTGATGAATTTATAA
ATTTTTTAAAAACCCCTGCTCCAAAGCAAAAAGAAGATCCGGTTAAATCAAGAGGCTCCATAACAATTTCTAGATCATTA
GAAGAATCAATTAAAATTGCAGATGCAATTATTGTTTTAAGACTCCAGAAAGAGCGAATGATGGAGAATTTACTAAGCAG
CATTGATTCATATAGTTTGGATTATGGCTTAACTCCAGAAAAATTATCTTTAAATAGTAAAGAAATTCCAATTCTTCATC
CTGGACCTATTAATAGAGGTATTGAAATCACAAGTAAAGTAGTAGATGAATATCCTAATTGCTTAATCAATAATCAAGTT
GCAAATGGTATCCCAATAAGAATGGCTTTACTTTATCTATTACAGAAATACAACAAATAA

Upstream 100 bases:

>100_bases
AAAATGGCGTTGGTATCTCAAAAATAGTAGAAGTGTAAGTAAAAGAATAAAAGGTGACAGAACACCAAAATTCAAATAAT
CATTTTTCTTTTTAAGCGTA

Downstream 100 bases:

>100_bases
TTCTATAGTAACTTAAGACTTTCAGTAAAGAAGCCATAAAATAATCCCAATCTTAAAGAATCTAATAAAAGTACTAAAAA
TTTCTTTTTCCTTTCAAATC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQTIWPHKHIHTLANFSIQDYKSVFELAHRFDVLKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFA
PSSSSLTKGETIIDTALTYSAMGADTLVIRHSSSYITFEIAKKLDAINAKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSK
KSLSPDVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKTLIPDEFINFLKTPAPKQKEDPVKSRGSITISRSL
EESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNSKEIPILHPGPINRGIEITSKVVDEYPNCLINNQV
ANGIPIRMALLYLLQKYNK

Sequences:

>Translated_339_residues
MQTIWPHKHIHTLANFSIQDYKSVFELAHRFDVLKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFA
PSSSSLTKGETIIDTALTYSAMGADTLVIRHSSSYITFEIAKKLDAINAKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSK
KSLSPDVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKTLIPDEFINFLKTPAPKQKEDPVKSRGSITISRSL
EESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNSKEIPILHPGPINRGIEITSKVVDEYPNCLINNQV
ANGIPIRMALLYLLQKYNK
>Mature_339_residues
MQTIWPHKHIHTLANFSIQDYKSVFELAHRFDVLKNAGTKKIPALQGTLVTSLFFEASTRTKNSFELAAKRLSADVQTFA
PSSSSLTKGETIIDTALTYSAMGADTLVIRHSSSYITFEIAKKLDAINAKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSK
KSLSPDVLNSKKILIIGDVNHSRVARSNLWALSAFGADIILCGPKTLIPDEFINFLKTPAPKQKEDPVKSRGSITISRSL
EESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNSKEIPILHPGPINRGIEITSKVVDEYPNCLINNQV
ANGIPIRMALLYLLQKYNK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=328, Percent_Identity=31.0975609756098, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI2367364, Length=333, Percent_Identity=29.4294294294294, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786469, Length=245, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790703, Length=246, Percent_Identity=28.0487804878049, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=30.7228915662651, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322331, Length=335, Percent_Identity=27.7611940298507, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=31.1377245508982, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PROM9 (Q31CU8)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396733.1
- HSSP:   P0A786
- ProteinModelPortal:   Q31CU8
- SMR:   Q31CU8
- STRING:   Q31CU8
- GeneID:   3765020
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0235
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- BioCyc:   PMAR74546:PMT9312_0235-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37525; Mature: 37525

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIWPHKHIHTLANFSIQDYKSVFELAHRFDVLKNAGTKKIPALQGTLVTSLFFEASTR
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
TKNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTALTYSAMGADTLVIRHSSSYITFEI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEHH
AKKLDAINAKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKKSLSPDVLNSKKILIIGDVN
HHHHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCC
HSRVARSNLWALSAFGADIILCGPKTLIPDEFINFLKTPAPKQKEDPVKSRGSITISRSL
HHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
EESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNSKEIPILHPGPINRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEECCCCCCCC
IEITSKVVDEYPNCLINNQVANGIPIRMALLYLLQKYNK
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTIWPHKHIHTLANFSIQDYKSVFELAHRFDVLKNAGTKKIPALQGTLVTSLFFEASTR
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
TKNSFELAAKRLSADVQTFAPSSSSLTKGETIIDTALTYSAMGADTLVIRHSSSYITFEI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEHH
AKKLDAINAKTSVLNAGDGLHSHPSQGLLDIYTLIKFFSKKSLSPDVLNSKKILIIGDVN
HHHHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCEEEEEECCC
HSRVARSNLWALSAFGADIILCGPKTLIPDEFINFLKTPAPKQKEDPVKSRGSITISRSL
HHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
EESIKIADAIIVLRLQKERMMENLLSSIDSYSLDYGLTPEKLSLNSKEIPILHPGPINRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEECCCCCCCC
IEITSKVVDEYPNCLINNQVANGIPIRMALLYLLQKYNK
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA