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The map label for this gene is pgk

Identifier: 78778583

GI number: 78778583

Start: 189482

End: 190690

Strand: Direct

Name: pgk

Synonym: PMT9312_0197

Alternate gene names: 78778583

Gene position: 189482-190690 (Clockwise)

Preceding gene: 78778580

Following gene: 78778585

Centisome position: 11.09

GC content: 36.56

Gene sequence:

>1209_bases
ATGTCAAAATTATCTCTTTCCAGTCTTGATAAGACAAATTTAGAAGGAAAAAAAGTTCTTGTAAGAGTAGATTTTAATGT
TCCATTAAATGAAGATGGTCAAATAACCGACGATACGCGTATTCGCGCAGCGATCCCAACTATTGAATATCTTATTAATC
ATTCTGCAAAAGTTATTTTAGCTGCTCATTTTGGTAGACCAAAAGGTCAGGTAAATGAAAAAATGAGATTAACTCCAGTA
GCAGCAAGATTAAGTGAATTGTTGGGGCAAAATGTTGCACTTACTAACAGTTGTATTGGTGATGAAGCAGTTGCACAATC
AAATAGCTTATCTAATGGAGATGTTCTTTTACTTGAAAATGTTCGTTTTTTTGGTGAAGAGGAAAAGAACGACTTGGAGT
TTGCTAAAAAATTAGCATCACATGCAGATATGTATGTAAATGATGCTTTCGGTGCTGCTCATAGAGCTCATGCGTCAACT
CAGGGAGTTACTAATTATTTAAGTCCCTCAGTAGCTGGATTCCTTTTAGAAAAAGAATTGAAATACTTACAGGGAGCAAT
AGATTCCCCAAAGCGTCCATTGGCAGCAATAGTTGGAGGATCAAAGGTTAGTAGCAAAATAGGAGTACTTGATTCTTTAC
TAGATAAGTGTGACAAAATCATGATTGGTGGAGGTATGATTTTCACTTTTTATAAAGCTAGAGGTTTAGATGTCGGAAAG
AGCCTTGTAGAAGAAGATAAACTCGAGCTTGCTAAAGATTTAGAAGCAAAAGCAAAAGCAAAAGGAGTAGAATTATTATT
ACCCACTGATGTTGTTTTAGCTGATGAATTTTCTCCGGACGCCAATAGTAAAATATCCCAAATTGATTCAATTAGTGGGA
ATTGGATGGGTCTCGATATTGGTCCAGATTCCATTAAAGTTTTTCAGAATGCTCTTGCAGAATGTAAGACAATTATTTGG
AATGGTCCAATGGGAGTTTTTGAATTTGATAAATTTGCAGACGGTACAAATGCAATAGCTACGACTCTTGCGGACTTAAG
TGCTTTTTCTGAAGTTTGTACAATAATTGGTGGTGGAGATTCAGTCGCAGCAGTAGAGAAAGCAGGATTAGCTGAGAAAA
TGTCTCATATATCTACTGGAGGTGGCGCAAGTTTGGAACTTTTAGAAGGTAAAACCTTACCAGGAGTAGCTGCGTTAAAC
GACGCTTAG

Upstream 100 bases:

>100_bases
TTCTTAAAATTTATTAAGGCATAGGTAACAGTAACTACTTTTTAACCCAATAAGCCCAAATTAACGTTAGGGTCTTAAGA
TTAAAAATTAAGCTTGTTTA

Downstream 100 bases:

>100_bases
GCTATATCTCATTAACAATATCAATGCTCTTTGTGAAAGTAATCATTCCCTCAGGGTGAGCTATTATTCCTGACCAAATT
TGTATCCCTGGTTTTGAAGG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSKLSLSSLDKTNLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVILAAHFGRPKGQVNEKMRLTPV
AARLSELLGQNVALTNSCIGDEAVAQSNSLSNGDVLLLENVRFFGEEEKNDLEFAKKLASHADMYVNDAFGAAHRAHAST
QGVTNYLSPSVAGFLLEKELKYLQGAIDSPKRPLAAIVGGSKVSSKIGVLDSLLDKCDKIMIGGGMIFTFYKARGLDVGK
SLVEEDKLELAKDLEAKAKAKGVELLLPTDVVLADEFSPDANSKISQIDSISGNWMGLDIGPDSIKVFQNALAECKTIIW
NGPMGVFEFDKFADGTNAIATTLADLSAFSEVCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKTLPGVAALN
DA

Sequences:

>Translated_402_residues
MSKLSLSSLDKTNLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVILAAHFGRPKGQVNEKMRLTPV
AARLSELLGQNVALTNSCIGDEAVAQSNSLSNGDVLLLENVRFFGEEEKNDLEFAKKLASHADMYVNDAFGAAHRAHAST
QGVTNYLSPSVAGFLLEKELKYLQGAIDSPKRPLAAIVGGSKVSSKIGVLDSLLDKCDKIMIGGGMIFTFYKARGLDVGK
SLVEEDKLELAKDLEAKAKAKGVELLLPTDVVLADEFSPDANSKISQIDSISGNWMGLDIGPDSIKVFQNALAECKTIIW
NGPMGVFEFDKFADGTNAIATTLADLSAFSEVCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKTLPGVAALN
DA
>Mature_401_residues
SKLSLSSLDKTNLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVILAAHFGRPKGQVNEKMRLTPVA
ARLSELLGQNVALTNSCIGDEAVAQSNSLSNGDVLLLENVRFFGEEEKNDLEFAKKLASHADMYVNDAFGAAHRAHASTQ
GVTNYLSPSVAGFLLEKELKYLQGAIDSPKRPLAAIVGGSKVSSKIGVLDSLLDKCDKIMIGGGMIFTFYKARGLDVGKS
LVEEDKLELAKDLEAKAKAKGVELLLPTDVVLADEFSPDANSKISQIDSISGNWMGLDIGPDSIKVFQNALAECKTIIWN
GPMGVFEFDKFADGTNAIATTLADLSAFSEVCTIIGGGDSVAAVEKAGLAEKMSHISTGGGASLELLEGKTLPGVAALND
A

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=423, Percent_Identity=45.3900709219858, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI31543397, Length=421, Percent_Identity=44.1805225653207, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1789294, Length=402, Percent_Identity=44.5273631840796, Blast_Score=313, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=47.1428571428571, Blast_Score=369, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319857, Length=418, Percent_Identity=50, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136394, Length=420, Percent_Identity=45.9523809523809, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24581203, Length=422, Percent_Identity=43.8388625592417, Blast_Score=328, Evalue=6e-90,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42669; Mature: 42538

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLSLSSLDKTNLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVIL
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEE
AAHFGRPKGQVNEKMRLTPVAARLSELLGQNVALTNSCIGDEAVAQSNSLSNGDVLLLEN
EEECCCCCCCCCCCEEEHHHHHHHHHHHCCCEEEHHCCCCCHHHHCCCCCCCCCEEEEEC
VRFFGEEEKNDLEFAKKLASHADMYVNDAFGAAHRAHASTQGVTNYLSPSVAGFLLEKEL
CHHCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
KYLQGAIDSPKRPLAAIVGGSKVSSKIGVLDSLLDKCDKIMIGGGMIFTFYKARGLDVGK
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCEEEECCCEEEEEEHHCCCHHHH
SLVEEDKLELAKDLEAKAKAKGVELLLPTDVVLADEFSPDANSKISQIDSISGNWMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCHHHHHHCCCCCEEEEEE
GPDSIKVFQNALAECKTIIWNGPMGVFEFDKFADGTNAIATTLADLSAFSEVCTIIGGGD
CCHHHHHHHHHHHHHHEEEECCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCC
SVAAVEKAGLAEKMSHISTGGGASLELLEGKTLPGVAALNDA
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLSLSSLDKTNLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLINHSAKVIL
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEE
AAHFGRPKGQVNEKMRLTPVAARLSELLGQNVALTNSCIGDEAVAQSNSLSNGDVLLLEN
EEECCCCCCCCCCCEEEHHHHHHHHHHHCCCEEEHHCCCCCHHHHCCCCCCCCCEEEEEC
VRFFGEEEKNDLEFAKKLASHADMYVNDAFGAAHRAHASTQGVTNYLSPSVAGFLLEKEL
CHHCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHH
KYLQGAIDSPKRPLAAIVGGSKVSSKIGVLDSLLDKCDKIMIGGGMIFTFYKARGLDVGK
HHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCEEEECCCEEEEEEHHCCCHHHH
SLVEEDKLELAKDLEAKAKAKGVELLLPTDVVLADEFSPDANSKISQIDSISGNWMGLDI
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCHHHHHHCCCCCEEEEEE
GPDSIKVFQNALAECKTIIWNGPMGVFEFDKFADGTNAIATTLADLSAFSEVCTIIGGGD
CCHHHHHHHHHHHHHHEEEECCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCC
SVAAVEKAGLAEKMSHISTGGGASLELLEGKTLPGVAALNDA
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA