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The map label for this gene is iscS [H]

Identifier: 78778558

GI number: 78778558

Start: 164451

End: 165596

Strand: Direct

Name: iscS [H]

Synonym: PMT9312_0172

Alternate gene names: 78778558

Gene position: 164451-165596 (Clockwise)

Preceding gene: 78778556

Following gene: 78778560

Centisome position: 9.62

GC content: 32.98

Gene sequence:

>1146_bases
ATGCTATCAACTCCCATACTACTAGACTACCAATCATCAACTCCTTGCTCTAAAGATGTTGTTGATTCTATGAAACCTTT
TTGGAGTGAGATATTTTCTAACCCTTCAAGCAAATCTAATTTGGCGGGTATAAACGCAAGCGCTATCTTGGAAGCCTCAA
GAGAAAGTATAGAAAAAACTTTATTTCTCCATAATAAAAAGGTTGTCTTTACAAGTGGGGCGACAGAATCTAATAACTTA
GCCTTAATAGGTTTTGCAAGAAATTACTATAAAAAAACAAGGCATTATGGACATATTATTACCTTAAAAACAGAGCATAA
AGCTGTATTGGAGCCCCTAAATCAACTAAAAAAAGAGGGATTTATGGTTACAGAAATCACTCCAGAAAGAGATGGGTTGA
TCTCAGAAGAAAAGTTCAAAAAGAACATAAGAGAAGATACATTTATGGTGAGTGTCATGTTGGCAAATAACGAAATAGGA
GTTATTCAGCCCATAAAGAATATTTCAAAGATATGTAACTCGAGAGGAATAGCTTTTCACTCAGATTTTGCACAATGTTT
AGGTTACATAGAGTTAAACGATCTTTTATCAAATGTAAATATGATAACGATGAGTTCTCATAAAATTTATGGCCCAAAAG
GGATAGGTCTTCTTTTGATTGATGAAGAAATTAATCTGGAACCTTTAATTGTTGGAGGAGGGCAGGAATATGGTCTAAGA
TCTGGCACATTACCTCTCCCTCTAGTAGTAGGCTTTGCTAAGGCAATAGAGATAGCCGTTCTTAATCAAAAAAAGAATGC
TGAAAAATTACTTTTGTATAGAAACAACCTTTTAAAGGGTTTGTTAGAAAATAATTCTGGTTTATTAATTAATGGCTCCA
TAAAAAAAAGGTTACCTCACAATTTGAATTTGACTGTATTGGATTTAAACGGAGCAAACTTTCATAAACTTTTAAAATCT
AAAATAATTTGTTCTAGTGGATCAGCATGCAGTAATGGTGAACCTTCTCACGTTTTACTCGCCTTAGGTAGATCTTTTAA
AGAAGCACAATCTTCAATAAGGCTAAGTATTGGATTAAGCACTAGTTCAAAAGATATAAAACAAGCAATTCAAATACTTA
CTAATACAATCAAAGCATTACGTTAG

Upstream 100 bases:

>100_bases
TTTTCTTACTGTTTCATTATTTATTTGATTAATTTCATTCATTTTTTAAATTCTTTAATTTGGTTTCTAGTAATAATGAG
AATTTTATATACTAAATACA

Downstream 100 bases:

>100_bases
GAATTATTGGCTCACTAATTTAATTGAGCAACTCTTAATTTTCCACTTCTAGCTCTTTTATTTATTTCTACTTCTTGTTC
GGAGGGAGTTATTGGCTTTT

Product: nifS-like aminotransferase class-V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLSTPILLDYQSSTPCSKDVVDSMKPFWSEIFSNPSSKSNLAGINASAILEASRESIEKTLFLHNKKVVFTSGATESNNL
ALIGFARNYYKKTRHYGHIITLKTEHKAVLEPLNQLKKEGFMVTEITPERDGLISEEKFKKNIREDTFMVSVMLANNEIG
VIQPIKNISKICNSRGIAFHSDFAQCLGYIELNDLLSNVNMITMSSHKIYGPKGIGLLLIDEEINLEPLIVGGGQEYGLR
SGTLPLPLVVGFAKAIEIAVLNQKKNAEKLLLYRNNLLKGLLENNSGLLINGSIKKRLPHNLNLTVLDLNGANFHKLLKS
KIICSSGSACSNGEPSHVLLALGRSFKEAQSSIRLSIGLSTSSKDIKQAIQILTNTIKALR

Sequences:

>Translated_381_residues
MLSTPILLDYQSSTPCSKDVVDSMKPFWSEIFSNPSSKSNLAGINASAILEASRESIEKTLFLHNKKVVFTSGATESNNL
ALIGFARNYYKKTRHYGHIITLKTEHKAVLEPLNQLKKEGFMVTEITPERDGLISEEKFKKNIREDTFMVSVMLANNEIG
VIQPIKNISKICNSRGIAFHSDFAQCLGYIELNDLLSNVNMITMSSHKIYGPKGIGLLLIDEEINLEPLIVGGGQEYGLR
SGTLPLPLVVGFAKAIEIAVLNQKKNAEKLLLYRNNLLKGLLENNSGLLINGSIKKRLPHNLNLTVLDLNGANFHKLLKS
KIICSSGSACSNGEPSHVLLALGRSFKEAQSSIRLSIGLSTSSKDIKQAIQILTNTIKALR
>Mature_381_residues
MLSTPILLDYQSSTPCSKDVVDSMKPFWSEIFSNPSSKSNLAGINASAILEASRESIEKTLFLHNKKVVFTSGATESNNL
ALIGFARNYYKKTRHYGHIITLKTEHKAVLEPLNQLKKEGFMVTEITPERDGLISEEKFKKNIREDTFMVSVMLANNEIG
VIQPIKNISKICNSRGIAFHSDFAQCLGYIELNDLLSNVNMITMSSHKIYGPKGIGLLLIDEEINLEPLIVGGGQEYGLR
SGTLPLPLVVGFAKAIEIAVLNQKKNAEKLLLYRNNLLKGLLENNSGLLINGSIKKRLPHNLNLTVLDLNGANFHKLLKS
KIICSSGSACSNGEPSHVLLALGRSFKEAQSSIRLSIGLSTSSKDIKQAIQILTNTIKALR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=37.5328083989501, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=30.7692307692308, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=39.6373056994819, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI25143064, Length=381, Percent_Identity=37.7952755905512, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=33.641975308642, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=41.8181818181818, Blast_Score=281, Evalue=9e-77,
Organism=Drosophila melanogaster, GI20129463, Length=381, Percent_Identity=38.3202099737533, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41919; Mature: 41919

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTPILLDYQSSTPCSKDVVDSMKPFWSEIFSNPSSKSNLAGINASAILEASRESIEKT
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LFLHNKKVVFTSGATESNNLALIGFARNYYKKTRHYGHIITLKTEHKAVLEPLNQLKKEG
HEEECCEEEEECCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
FMVTEITPERDGLISEEKFKKNIREDTFMVSVMLANNEIGVIQPIKNISKICNSRGIAFH
EEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHCCCCCEEE
SDFAQCLGYIELNDLLSNVNMITMSSHKIYGPKGIGLLLIDEEINLEPLIVGGGQEYGLR
HHHHHHHCHHHHHHHHCCCEEEEEECCEEECCCCCEEEEEECCCCCCEEEEECCCCCCCC
SGTLPLPLVVGFAKAIEIAVLNQKKNAEKLLLYRNNLLKGLLENNSGLLINGSIKKRLPH
CCCCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCC
NLNLTVLDLNGANFHKLLKSKIICSSGSACSNGEPSHVLLALGRSFKEAQSSIRLSIGLS
CCCEEEEEECCCHHHHHHHHHHEECCCCCCCCCCCCEEEEEHHCHHHHHHCCEEEEEECC
TSSKDIKQAIQILTNTIKALR
CCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSTPILLDYQSSTPCSKDVVDSMKPFWSEIFSNPSSKSNLAGINASAILEASRESIEKT
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LFLHNKKVVFTSGATESNNLALIGFARNYYKKTRHYGHIITLKTEHKAVLEPLNQLKKEG
HEEECCEEEEECCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
FMVTEITPERDGLISEEKFKKNIREDTFMVSVMLANNEIGVIQPIKNISKICNSRGIAFH
EEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHCCCCCEEE
SDFAQCLGYIELNDLLSNVNMITMSSHKIYGPKGIGLLLIDEEINLEPLIVGGGQEYGLR
HHHHHHHCHHHHHHHHCCCEEEEEECCEEECCCCCEEEEEECCCCCCEEEEECCCCCCCC
SGTLPLPLVVGFAKAIEIAVLNQKKNAEKLLLYRNNLLKGLLENNSGLLINGSIKKRLPH
CCCCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCC
NLNLTVLDLNGANFHKLLKSKIICSSGSACSNGEPSHVLLALGRSFKEAQSSIRLSIGLS
CCCEEEEEECCCHHHHHHHHHHEECCCCCCCCCCCCEEEEEHHCHHHHHHCCEEEEEECC
TSSKDIKQAIQILTNTIKALR
CCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA