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The map label for this gene is ppnK

Identifier: 78778544

GI number: 78778544

Start: 153118

End: 154029

Strand: Reverse

Name: ppnK

Synonym: PMT9312_0158

Alternate gene names: 78778544

Gene position: 154029-153118 (Counterclockwise)

Preceding gene: 78778545

Following gene: 78778543

Centisome position: 9.01

GC content: 29.71

Gene sequence:

>912_bases
ATGAAACTTTCATTAGTGCTTATTATATATCGTTCAGATAGTTCTATCGCTTTAGAGGCTTCTAAATTCTGTGAAGAGGT
CCTCAAAGCAAAAAATATTAAATCAAACAGGATTGCAAGTGATTTTCATAGAGATGAAATTGAAAAAAATCTTTATAATA
CAAAATTTCAACCAGATATTGGAATCGTTCTTGGTGGGGATGGAACCTTCCTAAAATGTGCAAATGCATTAGCAGATTAT
GATATTCCTTTATTGAGCATTAATATTGGTGGTAATTTGGGGTTTCTTACTCAGGAAAAAGATTTTTTATTTGACAAATC
TTTTATTGAAATCCTTGAAAACGAAGAATATATAATTGATTCTCGTAATAGATTAAATTGTAATGTCTGTATTAGTGAAA
GAAGTCCTGAGAAAAAAATTATAAAAAGCTACGACGCATTAAATGATTTTTATTTTAAATCAGTGGAAGAGGCTATTTCT
CCTACGAACCAAATACAAATTGAAATCGATAATGAGAAAGTGAATGAATATAAAGGTGATGGATTGATTATATCTACTTC
TACTGGTTCAACAGCATACTCAATGGCTGCAGGTGGTCCAATAGTACATCCTAGTATAGATGCAATGATAATTAACCCTA
TTTGCCCAATGAGTTTAGCTAGTAGACCAATTGTCATACCTAATACAAGTAAAGTGATCATTAAACCAGTAAAAAAAAGT
AAAGGGGAAATTAAATTATGGCGAGATGGTTCAAAATGTATGACCATTAAGGAAACTTATTATTGTGAGATCAAAAAAGG
GAAAGCACCCTGCAAAATAATAAAGTTTAAAAAAAGCACTAACTATTACAATACTTTAATAAAAAAACTAGATTGGAAAG
GTGATTTATCTCTAAAAAATTCCAAAAATTAA

Upstream 100 bases:

>100_bases
AGAAGCAGCCGTTGGCTTAGCTATACTGCTATCGCTTTACAGAAATAGGGTTACTGTAGATATGGAAAGTTTTAATTTAT
TAAAATGGTAAAAGCATTAA

Downstream 100 bases:

>100_bases
ATGGCCTTAGAAATAGAAAGACGCTTTCTTATAAAAAATGATAACTGGAAAGAATTCATCACTAAAAAAATCTTTATTGA
ACAAGGATATTTATCAAAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKLSLVLIIYRSDSSIALEASKFCEEVLKAKNIKSNRIASDFHRDEIEKNLYNTKFQPDIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILENEEYIIDSRNRLNCNVCISERSPEKKIIKSYDALNDFYFKSVEEAIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKETYYCEIKKGKAPCKIIKFKKSTNYYNTLIKKLDWKGDLSLKNSKN

Sequences:

>Translated_303_residues
MKLSLVLIIYRSDSSIALEASKFCEEVLKAKNIKSNRIASDFHRDEIEKNLYNTKFQPDIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILENEEYIIDSRNRLNCNVCISERSPEKKIIKSYDALNDFYFKSVEEAIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKETYYCEIKKGKAPCKIIKFKKSTNYYNTLIKKLDWKGDLSLKNSKN
>Mature_303_residues
MKLSLVLIIYRSDSSIALEASKFCEEVLKAKNIKSNRIASDFHRDEIEKNLYNTKFQPDIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILENEEYIIDSRNRLNCNVCISERSPEKKIIKSYDALNDFYFKSVEEAIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKETYYCEIKKGKAPCKIIKFKKSTNYYNTLIKKLDWKGDLSLKNSKN

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788968, Length=238, Percent_Identity=34.8739495798319, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320794, Length=246, Percent_Identity=30.0813008130081, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=247, Percent_Identity=29.5546558704453, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325068, Length=178, Percent_Identity=33.7078651685393, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573828, Length=293, Percent_Identity=27.9863481228669, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573830, Length=293, Percent_Identity=27.9863481228669, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161077047, Length=293, Percent_Identity=27.9863481228669, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573826, Length=293, Percent_Identity=27.9863481228669, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573832, Length=293, Percent_Identity=27.9863481228669, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653422, Length=271, Percent_Identity=25.830258302583, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129957, Length=271, Percent_Identity=25.830258302583, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363321, Length=271, Percent_Identity=25.830258302583, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363323, Length=271, Percent_Identity=25.830258302583, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653424, Length=271, Percent_Identity=25.830258302583, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_PROM9 (Q31D25)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396656.1
- ProteinModelPortal:   Q31D25
- SMR:   Q31D25
- STRING:   Q31D25
- GeneID:   3764943
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0158
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   YDIPLLS
- ProtClustDB:   PRK02645
- BioCyc:   PMAR74546:PMT9312_0158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 34066; Mature: 34066

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLVLIIYRSDSSIALEASKFCEEVLKAKNIKSNRIASDFHRDEIEKNLYNTKFQPDI
CCEEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
GIVLGGDGTFLKCANALADYDIPLLSINIGGNLGFLTQEKDFLFDKSFIEILENEEYIID
EEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCEEEEE
SRNRLNCNVCISERSPEKKIIKSYDALNDFYFKSVEEAISPTNQIQIEIDNEKVNEYKGD
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCC
GLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
EEEEEECCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEEECCCCEEEEEECCCC
KGEIKLWRDGSKCMTIKETYYCEIKKGKAPCKIIKFKKSTNYYNTLIKKLDWKGDLSLKN
CCCEEEEECCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCC
SKN
CCC
>Mature Secondary Structure
MKLSLVLIIYRSDSSIALEASKFCEEVLKAKNIKSNRIASDFHRDEIEKNLYNTKFQPDI
CCEEEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
GIVLGGDGTFLKCANALADYDIPLLSINIGGNLGFLTQEKDFLFDKSFIEILENEEYIID
EEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCEEEEE
SRNRLNCNVCISERSPEKKIIKSYDALNDFYFKSVEEAISPTNQIQIEIDNEKVNEYKGD
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCC
GLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
EEEEEECCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEEECCCCEEEEEECCCC
KGEIKLWRDGSKCMTIKETYYCEIKKGKAPCKIIKFKKSTNYYNTLIKKLDWKGDLSLKN
CCCEEEEECCCEEEEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCC
SKN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA