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The map label for this gene is radA [H]

Identifier: 78778521

GI number: 78778521

Start: 131196

End: 132545

Strand: Reverse

Name: radA [H]

Synonym: PMT9312_0135

Alternate gene names: 78778521

Gene position: 132545-131196 (Counterclockwise)

Preceding gene: 78778527

Following gene: 78778519

Centisome position: 7.75

GC content: 32.81

Gene sequence:

>1350_bases
ATGTCTAGCAAATTGTCAACTTTTATTTGTCAAAATTGCGGAACTGAAACTTCTCAATACTTTGGGAAATGCCTTAATTG
CAACTCATGGAATTCCATTGTTGAAGAAATTAAAAGCAAGAGATCTAAGCATCAAGGTGTAAATAATAGTCAAAAAGCTA
TACCATTTAATGAAATTTCATCTAAAAAAATATCAAGATTTACAAGTGGTTTTAATGAGTTTGATCGAGTTCTTGGAGGC
GGGATAGTCCCTGGATCTGTTGTGTTACTTGGAGGAGAGCCAGGGATAGGTAAAAGCACAATAGTTCTTCAATCAGCAGG
AAAAATATCTCTCAACGAGAAAGTTTTATACGTAACTGCCGAGGAATCTTTAGAACAAGTAAAAATCAGATGGGAAAGAT
TAAATCAAAATAGTATAGATTTAAAAATTTTTGCAGAAACCAATTTATCTCTAATAATTAAAGAGATAAAACGTGTAAAT
CCAAGTTTCGCAATTATCGATAGTATTCAAGCCATCCATAATCATGAGATGGAAAGTTCGCCAGGATCAGTTTCTCAAGT
TAGAGAATGTTCATCTGAATTACAGAATCTCGCTAAACAAAATAATATTGCACTTCTAATAATTGGTCATGTCACCAAAG
ATGGGACTTTAGCTGGTCCAAAAACTCTAGAGCATTTAGTTGATGCAGTAATAAACTTTGAAGGAGATAATATTTCCTCT
CATAGATTACTAAGAAGTATAAAAAATCGATTTGGATCTACCTTTGAAATTGGAATTTTTGAAATGCTTGAAAAAGGCTT
ACGGGAGGTAAAAAACCCAAGTTCAATCTTTACAAATAAAGAGAATATTGCAGGTGTGACAACCACGATTACAAAAGAAG
GTACTAGACCATTTGCAGTTGATATACAAGCACTGGTAAATAAAACTTTCTACAGTAACCCAAGACGAACCACAACTGGA
ATTAGCATTAACAGATTACATCAAATTCTAGCTGTTATTGAAAAACACGTCGGCATTAAATTATCTGAATTTGATTGTTA
TGTAGCTACTGGGGGTGGTTTTGAAATTAATGATCCTTCATCTGACTTAGGTGTAGCCATATCAATTTTATCAAGTTTAA
AAAATATTCCTCCTTTGGCCAATAGCTCATTTATTGGAGAATTGGGTTTGAGCGGTCAAGTTAGAAAATCGAATAACCTT
CGAACAAAGATAGAAGAGGCTGCAAGACTAGGAATCAAAAATATCGTAGTGCCAAAATTAGAGGAGGATCTAAATAATTT
TCAAAATTTAATCAATATCAAAGAGATTTCGAATATTAAAGAAGCAGTTGAATATTCTTTATCAGAGTAA

Upstream 100 bases:

>100_bases
TATTAGCTAATCAGTATGAGAATTTGTTATTAACCTAATCTAACTTGTAGAGGGGCAATATCCCAAACATTCTAGTTCCA
TGTACTTTTTTAAAAACTAA

Downstream 100 bases:

>100_bases
AAATATTAGAGATACATATCAATTGAACTTCTTCCTGAGTTTTTCAGCCAATTCTCAATATCAAGATACCCCCCAGGATA
TAATTTTACTGCTTTAGTCC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSSKLSTFICQNCGTETSQYFGKCLNCNSWNSIVEEIKSKRSKHQGVNNSQKAIPFNEISSKKISRFTSGFNEFDRVLGG
GIVPGSVVLLGGEPGIGKSTIVLQSAGKISLNEKVLYVTAEESLEQVKIRWERLNQNSIDLKIFAETNLSLIIKEIKRVN
PSFAIIDSIQAIHNHEMESSPGSVSQVRECSSELQNLAKQNNIALLIIGHVTKDGTLAGPKTLEHLVDAVINFEGDNISS
HRLLRSIKNRFGSTFEIGIFEMLEKGLREVKNPSSIFTNKENIAGVTTTITKEGTRPFAVDIQALVNKTFYSNPRRTTTG
ISINRLHQILAVIEKHVGIKLSEFDCYVATGGGFEINDPSSDLGVAISILSSLKNIPPLANSSFIGELGLSGQVRKSNNL
RTKIEEAARLGIKNIVVPKLEEDLNNFQNLINIKEISNIKEAVEYSLSE

Sequences:

>Translated_449_residues
MSSKLSTFICQNCGTETSQYFGKCLNCNSWNSIVEEIKSKRSKHQGVNNSQKAIPFNEISSKKISRFTSGFNEFDRVLGG
GIVPGSVVLLGGEPGIGKSTIVLQSAGKISLNEKVLYVTAEESLEQVKIRWERLNQNSIDLKIFAETNLSLIIKEIKRVN
PSFAIIDSIQAIHNHEMESSPGSVSQVRECSSELQNLAKQNNIALLIIGHVTKDGTLAGPKTLEHLVDAVINFEGDNISS
HRLLRSIKNRFGSTFEIGIFEMLEKGLREVKNPSSIFTNKENIAGVTTTITKEGTRPFAVDIQALVNKTFYSNPRRTTTG
ISINRLHQILAVIEKHVGIKLSEFDCYVATGGGFEINDPSSDLGVAISILSSLKNIPPLANSSFIGELGLSGQVRKSNNL
RTKIEEAARLGIKNIVVPKLEEDLNNFQNLINIKEISNIKEAVEYSLSE
>Mature_448_residues
SSKLSTFICQNCGTETSQYFGKCLNCNSWNSIVEEIKSKRSKHQGVNNSQKAIPFNEISSKKISRFTSGFNEFDRVLGGG
IVPGSVVLLGGEPGIGKSTIVLQSAGKISLNEKVLYVTAEESLEQVKIRWERLNQNSIDLKIFAETNLSLIIKEIKRVNP
SFAIIDSIQAIHNHEMESSPGSVSQVRECSSELQNLAKQNNIALLIIGHVTKDGTLAGPKTLEHLVDAVINFEGDNISSH
RLLRSIKNRFGSTFEIGIFEMLEKGLREVKNPSSIFTNKENIAGVTTTITKEGTRPFAVDIQALVNKTFYSNPRRTTTGI
SINRLHQILAVIEKHVGIKLSEFDCYVATGGGFEINDPSSDLGVAISILSSLKNIPPLANSSFIGELGLSGQVRKSNNLR
TKIEEAARLGIKNIVVPKLEEDLNNFQNLINIKEISNIKEAVEYSLSE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=426, Percent_Identity=42.2535211267606, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49347; Mature: 49216

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLSTFICQNCGTETSQYFGKCLNCNSWNSIVEEIKSKRSKHQGVNNSQKAIPFNEIS
CCCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
SKKISRFTSGFNEFDRVLGGGIVPGSVVLLGGEPGIGKSTIVLQSAGKISLNEKVLYVTA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEECCCCEEECCEEEEEEH
EESLEQVKIRWERLNQNSIDLKIFAETNLSLIIKEIKRVNPSFAIIDSIQAIHNHEMESS
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
PGSVSQVRECSSELQNLAKQNNIALLIIGHVTKDGTLAGPKTLEHLVDAVINFEGDNISS
CCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHH
HRLLRSIKNRFGSTFEIGIFEMLEKGLREVKNPSSIFTNKENIAGVTTTITKEGTRPFAV
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCEE
DIQALVNKTFYSNPRRTTTGISINRLHQILAVIEKHVGIKLSEFDCYVATGGGFEINDPS
EHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEEEECCCEEECCCC
SDLGVAISILSSLKNIPPLANSSFIGELGLSGQVRKSNNLRTKIEEAARLGIKNIVVPKL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCHHHCCCCHH
EEDLNNFQNLINIKEISNIKEAVEYSLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSKLSTFICQNCGTETSQYFGKCLNCNSWNSIVEEIKSKRSKHQGVNNSQKAIPFNEIS
CCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
SKKISRFTSGFNEFDRVLGGGIVPGSVVLLGGEPGIGKSTIVLQSAGKISLNEKVLYVTA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEECCCCEEECCEEEEEEH
EESLEQVKIRWERLNQNSIDLKIFAETNLSLIIKEIKRVNPSFAIIDSIQAIHNHEMESS
HHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
PGSVSQVRECSSELQNLAKQNNIALLIIGHVTKDGTLAGPKTLEHLVDAVINFEGDNISS
CCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHH
HRLLRSIKNRFGSTFEIGIFEMLEKGLREVKNPSSIFTNKENIAGVTTTITKEGTRPFAV
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCEE
DIQALVNKTFYSNPRRTTTGISINRLHQILAVIEKHVGIKLSEFDCYVATGGGFEINDPS
EHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEEEEECCCEEECCCC
SDLGVAISILSSLKNIPPLANSSFIGELGLSGQVRKSNNLRTKIEEAARLGIKNIVVPKL
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCHHHCCCCHH
EEDLNNFQNLINIKEISNIKEAVEYSLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]