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The map label for this gene is dus2 [H]

Identifier: 78778399

GI number: 78778399

Start: 16740

End: 17744

Strand: Reverse

Name: dus2 [H]

Synonym: PMT9312_0014

Alternate gene names: 78778399

Gene position: 17744-16740 (Counterclockwise)

Preceding gene: 78778405

Following gene: 78778392

Centisome position: 1.04

GC content: 30.25

Gene sequence:

>1005_bases
ATGAGTTCTATTAAATCTAATTCTATTAAAAATATTCATAAATTAAGTATTGCTCCAATGATGGATTGTACTGATAAACA
TTTCAGAATGATAATGAGAAAAATAAGTTCTAAAGCTCTCCTATATACAGAAATGATTGTGGCCCAAAGTTTAATTTATA
CCAATGAGAAAGAAAAATTTTTAGATTTTAATAATGAAGAACATCCGATCTCTATTCAATTTGGTGGGGATAATCCTAAA
ATCCTTAAAGAGGGTGCCAAAATGGCACAAGATTGGGGTTATGACGAAATAAACTTTAATGTTGGTTGTCCAAGTCCAAG
AGTCTGTTCAGGCAATTTTGGCGCTTCACTTATGAAAGATCCTGAAAAAGTAGCAAAATGCATAGAATCCTTAAAAAATA
GCTGTAGCTTACCGGTTACAATTAAACACAGAATCGGTGTAGACGAAGATGATAGTTTCGTAAATTTAAATAATTTTGTA
CGATACATAGCAAATGCTGGCGCAGACAGATTTACAGTTCATGCAAGGAAAGCCATATTAAAAGGTCTAAATCCAAAACA
AAACAGAACTATTCCTCCACTTAAATATGATGTAGTAAAAAAATTAAAAAAATTAAATCCAGAATTGTTAATAGAAATCA
ATGGAGGTTTCACAAATATCAATGAATCATTAGAAGCCCTAAGTAATTTTGATGGTGTAATGATTGGACGATCAGTATAT
AAACATCCCTTAAGATGGTCTGAAATTGATCAAAAAGTTTATGGGATCAATACAAAACCCAAATCTGCTTCAGATATCAT
CTTCTCCTTAATTCCATACATAGAGGAACATTTAAGTAATGGAGGTAAATCTTGGGATATATGTAAACATCTTATTAATT
TAGTCGAAGGTATTCCAAAAGCTAAAGTTTGGAGAAATCAAATTTCAACTAAATCTATAAAAAAGGAATTAGATATTGAT
TATCTTCTTAAGTTAACTTCTAGACTTGAAGAAATGGGTTATTAA

Upstream 100 bases:

>100_bases
ATAGGCTGAAAGATTATTTTTAAAATTGACATAGTAGGAATTAAAATGAAAGTTCGTCTTGAAAAAAATTGATTCATATA
TTTAAATCACATAAAAAGTA

Downstream 100 bases:

>100_bases
TTCTCTTCATTTGATAAATTACTCTCGTTTGAAACTCCTCTTTTTCTCCTACTTCTGCCACTTTCATATTCAGAGTTTTC
AGAAGAGTTTCCATAACTTC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSSIKSNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSKALLYTEMIVAQSLIYTNEKEKFLDFNNEEHPISIQFGGDNPK
ILKEGAKMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNSCSLPVTIKHRIGVDEDDSFVNLNNFV
RYIANAGADRFTVHARKAILKGLNPKQNRTIPPLKYDVVKKLKKLNPELLIEINGGFTNINESLEALSNFDGVMIGRSVY
KHPLRWSEIDQKVYGINTKPKSASDIIFSLIPYIEEHLSNGGKSWDICKHLINLVEGIPKAKVWRNQISTKSIKKELDID
YLLKLTSRLEEMGY

Sequences:

>Translated_334_residues
MSSIKSNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSKALLYTEMIVAQSLIYTNEKEKFLDFNNEEHPISIQFGGDNPK
ILKEGAKMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNSCSLPVTIKHRIGVDEDDSFVNLNNFV
RYIANAGADRFTVHARKAILKGLNPKQNRTIPPLKYDVVKKLKKLNPELLIEINGGFTNINESLEALSNFDGVMIGRSVY
KHPLRWSEIDQKVYGINTKPKSASDIIFSLIPYIEEHLSNGGKSWDICKHLINLVEGIPKAKVWRNQISTKSIKKELDID
YLLKLTSRLEEMGY
>Mature_333_residues
SSIKSNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSKALLYTEMIVAQSLIYTNEKEKFLDFNNEEHPISIQFGGDNPKI
LKEGAKMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNSCSLPVTIKHRIGVDEDDSFVNLNNFVR
YIANAGADRFTVHARKAILKGLNPKQNRTIPPLKYDVVKKLKKLNPELLIEINGGFTNINESLEALSNFDGVMIGRSVYK
HPLRWSEIDQKVYGINTKPKSASDIIFSLIPYIEEHLSNGGKSWDICKHLINLVEGIPKAKVWRNQISTKSIKKELDIDY
LLKLTSRLEEMGY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=232, Percent_Identity=26.2931034482759, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40807366, Length=172, Percent_Identity=29.6511627906977, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=41.5094339622642, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789660, Length=242, Percent_Identity=24.7933884297521, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788462, Length=242, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=169, Percent_Identity=25.4437869822485, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=170, Percent_Identity=30.5882352941176, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=30.9941520467836, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=30.9941520467836, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37853; Mature: 37722

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIKSNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSKALLYTEMIVAQSLIYTNEKEKF
CCCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHH
LDFNNEEHPISIQFGGDNPKILKEGAKMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
HCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKNSCSLPVTIKHRIGVDEDDSFVNLNNFVRYIANAGADRFTVHARKAIL
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECHHHHHHHHHHCCCCHHHHHHHHHHH
KGLNPKQNRTIPPLKYDVVKKLKKLNPELLIEINGGFTNINESLEALSNFDGVMIGRSVY
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHCCHHHH
KHPLRWSEIDQKVYGINTKPKSASDIIFSLIPYIEEHLSNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
AKVWRNQISTKSIKKELDIDYLLKLTSRLEEMGY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSIKSNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSKALLYTEMIVAQSLIYTNEKEKF
CCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHH
LDFNNEEHPISIQFGGDNPKILKEGAKMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
HCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKNSCSLPVTIKHRIGVDEDDSFVNLNNFVRYIANAGADRFTVHARKAIL
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECHHHHHHHHHHCCCCHHHHHHHHHHH
KGLNPKQNRTIPPLKYDVVKKLKKLNPELLIEINGGFTNINESLEALSNFDGVMIGRSVY
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHCCHHHH
KHPLRWSEIDQKVYGINTKPKSASDIIFSLIPYIEEHLSNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCH
AKVWRNQISTKSIKKELDIDYLLKLTSRLEEMGY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]