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The map label for this gene is fliY [H]

Identifier: 78358781

GI number: 78358781

Start: 3697260

End: 3698045

Strand: Direct

Name: fliY [H]

Synonym: Dde_3742

Alternate gene names: 78358781

Gene position: 3697260-3698045 (Clockwise)

Preceding gene: 78358780

Following gene: 78358782

Centisome position: 99.12

GC content: 57.25

Gene sequence:

>786_bases
ATGAAGATTGCATTCCGTTATTTTCCGCTGGCTGCCGTGCTTATGCTCGCCCTTCTGCTTTCCGCCTGTTCGGAAGAAAA
AACCGACGCGCTCAGCCGCATCAAGGAAGCGGGCGAAATCAGCTTTGCCATGAGCGGCGGTTACCCGCCCTTCAATTTTT
ACAATGACAGCAACGAGCTGACAGGCTTTGACGTTGACGTTGCCAAAGAGGTGGCATCGCGCCTCGGCGTTGCCTTTGTA
CCCGTGACCACAGAGTGGAGCGGGATCATTGAAGGACTGCGCTCCGGCGCCTACGACGGCATTCTTGGCAGCATGGCTGT
CACCCCCGAACGCCTTAAGGTGGTAAACTTTTCAAACGCCTACTACTACTCTGGCGCGCAGGTAATGGTAAAAGACAGTG
CCCCTTACACCGCACCGCAGGAGCTGAAAGGCAAGGTTTTCGGCGTGGTGACCGGCACAACGTTTGCCGAAGATGCCGAA
AAGCTGGGCGCCGGCGAAGTGCGTCTGTACAAAGATGACACACAGACCCTGCTTGAGCTTGATAACGGCGTGGTCGACGC
CGTCATCACCGACAGGGTTGTGGGTGTGAACGCCGTCAATTCCGGCAGATTCAACATCCGCATGCTGGGCGCCCCGCTGC
GCAGCGAAAATATCGCCGTCGCCTTCCGCAAAGGCGAAGACACCCTGCTTAAAGAAGTCAACGCCGTACTTGCCGCCATG
CACGAAGACGGCACGCTGGCCAGTCTGAGCCGCAAATGGCTTAACGCCGACATAACCACCCGCTAG

Upstream 100 bases:

>100_bases
GCATATCTGCCTGCTGCCGGAGACCTGCGTACATAAAGAGCTGTTTGCCGTTTTTATCCATACGCACAAGGGCACAACAT
AAACCTTACCGGAGTTTACC

Downstream 100 bases:

>100_bases
CGCGTAGAAACGCTTTTACCGCCGCACACTACCCTCAGGCAGTGTGCGGCGGCTGCCCTGCCGTACAAACAACAACACAC
CGGAAATATTATGTACTTCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKIAFRYFPLAAVLMLALLLSACSEEKTDALSRIKEAGEISFAMSGGYPPFNFYNDSNELTGFDVDVAKEVASRLGVAFV
PVTTEWSGIIEGLRSGAYDGILGSMAVTPERLKVVNFSNAYYYSGAQVMVKDSAPYTAPQELKGKVFGVVTGTTFAEDAE
KLGAGEVRLYKDDTQTLLELDNGVVDAVITDRVVGVNAVNSGRFNIRMLGAPLRSENIAVAFRKGEDTLLKEVNAVLAAM
HEDGTLASLSRKWLNADITTR

Sequences:

>Translated_261_residues
MKIAFRYFPLAAVLMLALLLSACSEEKTDALSRIKEAGEISFAMSGGYPPFNFYNDSNELTGFDVDVAKEVASRLGVAFV
PVTTEWSGIIEGLRSGAYDGILGSMAVTPERLKVVNFSNAYYYSGAQVMVKDSAPYTAPQELKGKVFGVVTGTTFAEDAE
KLGAGEVRLYKDDTQTLLELDNGVVDAVITDRVVGVNAVNSGRFNIRMLGAPLRSENIAVAFRKGEDTLLKEVNAVLAAM
HEDGTLASLSRKWLNADITTR
>Mature_261_residues
MKIAFRYFPLAAVLMLALLLSACSEEKTDALSRIKEAGEISFAMSGGYPPFNFYNDSNELTGFDVDVAKEVASRLGVAFV
PVTTEWSGIIEGLRSGAYDGILGSMAVTPERLKVVNFSNAYYYSGAQVMVKDSAPYTAPQELKGKVFGVVTGTTFAEDAE
KLGAGEVRLYKDDTQTLLELDNGVVDAVITDRVVGVNAVNSGRFNIRMLGAPLRSENIAVAFRKGEDTLLKEVNAVLAAM
HEDGTLASLSRKWLNADITTR

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=255, Percent_Identity=35.2941176470588, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787085, Length=228, Percent_Identity=30.2631578947368, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=28.6290322580645, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788648, Length=223, Percent_Identity=27.3542600896861, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788649, Length=223, Percent_Identity=23.3183856502242, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28198; Mature: 28198

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFRYFPLAAVLMLALLLSACSEEKTDALSRIKEAGEISFAMSGGYPPFNFYNDSNEL
CEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
TGFDVDVAKEVASRLGVAFVPVTTEWSGIIEGLRSGAYDGILGSMAVTPERLKVVNFSNA
CCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEECCE
YYYSGAQVMVKDSAPYTAPQELKGKVFGVVTGTTFAEDAEKLGAGEVRLYKDDTQTLLEL
EEECCCEEEEECCCCCCCCHHHCCEEEEEEECCCHHHHHHHCCCCEEEEEECCHHHHHHH
DNGVVDAVITDRVVGVNAVNSGRFNIRMLGAPLRSENIAVAFRKGEDTLLKEVNAVLAAM
CCCEEEHHHHCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
HEDGTLASLSRKWLNADITTR
CCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIAFRYFPLAAVLMLALLLSACSEEKTDALSRIKEAGEISFAMSGGYPPFNFYNDSNEL
CEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
TGFDVDVAKEVASRLGVAFVPVTTEWSGIIEGLRSGAYDGILGSMAVTPERLKVVNFSNA
CCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEECCE
YYYSGAQVMVKDSAPYTAPQELKGKVFGVVTGTTFAEDAEKLGAGEVRLYKDDTQTLLEL
EEECCCEEEEECCCCCCCCHHHCCEEEEEEECCCHHHHHHHCCCCEEEEEECCHHHHHHH
DNGVVDAVITDRVVGVNAVNSGRFNIRMLGAPLRSENIAVAFRKGEDTLLKEVNAVLAAM
CCCEEEHHHHCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
HEDGTLASLSRKWLNADITTR
CCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]