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The map label for this gene is mltD [H]

Identifier: 78358619

GI number: 78358619

Start: 3539285

End: 3540919

Strand: Reverse

Name: mltD [H]

Synonym: Dde_3580

Alternate gene names: 78358619

Gene position: 3540919-3539285 (Counterclockwise)

Preceding gene: 78358621

Following gene: 78358614

Centisome position: 94.92

GC content: 57.74

Gene sequence:

>1635_bases
ATGCATATATTCTCACGCGGTTTTGCTCCGGGCCGCGCCGGCGTGCTGGCACTGCTTGTGCTCATGCTCGCGGGTGGCGG
CTGCGTGTCCAAAAATTCTCCGCAACCCGAACTGCCCCCCGTAGCCGAAGTGCAGGCCTGCCCCGCACCGCTGCAAGAGC
AGGATGCTTCCATCAGCGATGCCGAAGAAGTGGTTGAAATTGAAGTGCCGCCGGCAGACGACAATCAGCCTCTGACCGTG
GAAGAAGAGCAGGCTCTGCTGACCCAGCTGGATATTGATATTGAAATGGACGAAAGCGACCGCAGCGTGGTCGAGTCGTA
CATCAAATATTTCACCCACCGTGCCCGCGGTTCGTTTGAGCGCTACCTCGAACGCGCCGAAGTGTATCTGCCCGCCGCAA
GAGAAGTCTTCCGGCAGAAGGGGCTGCCTGAAGAACTGGTCTATCTTGCATTTGTGGAAAGCGGCTTCAACCCCAACGCG
TACTCGCGGGCCGGTGCCGCCGGTGTGTGGCAGTTTATGCCTTTCACCGGCCGCAAATACGGGCTGCGGTACGACTGGTG
GATTGATGAACGGCGCGATCCTTATAAGTCGGCAGAGGCTGCGGCCAACTACCTGTCGTGGCTTTATGCAGAATTCGGCG
ACTGGTATCTGGCTCTTGCCGCGTATAATGCAGGTGAGGGCAAGATAGGCAGAGCCATAGCAGGCACCGGTGCCGAGAGC
TTTTTTGAACTTACAAGCCAGAATCATAAACTGAGCCACCGTAAGCAGCTTCGCCGTGAGACGCGTCACTATGTGCCTAA
GTTCATAGCCATAGTGAAGATCATGCGTAACCTTGAAGAGCTTGGTTTCAAACCGCTGCGGCTTGATTGCGCTCCCGAGC
TGAATCCGCTGCAGGTAAAGGGTGGCACCGATTTGCTGGCACTGGCCGATGCCGCCGGAATGAAATGGGACGACTTTAAG
AAATACAACACCGCGTTTCTGCGGTATGTCACGCCTCCCGATGCTGAGACCACTGTATATGTGACTCCCGCGGCAAAGTC
CGGTGCTCTGGCGTTTCTGGCAAAGCCGGAATCACGCCCGTATGCCGGATGGGCCGATTACAAGGTGCGCAGCGGTGACT
CATGGTATCGTATCAGCCGCAGATACGGGGTGCCTGTGTCTGTGCTGAAAAAGGTCAACCAGAAGAAAAGCAATCTGCTG
CGTCCGGGTGAGCGGCTCATGATTCCGCGTACCGGCAGTGGCAGAATTCCTTCTTCGCCCATGCAGGAGACCAGAGCACT
GGCGCAGAAGCGCGGTGACTACGTGGTGAGTCAGGGCGACACCCTGTACGATATAGCCCGCAACTACGGGCTGACAGTGG
CCACGCTGAAGCAGGCCAACGGTCTGCGCAGCAGCATGCTGCGTCCCGGACAGAAGCTGTACATTCCCGGACAGACCAGA
GCGCAGGAAGCCCGTACCCGCAAGGACGCGGAACAGGCGCGCAAGGCCGTTGTGTACAGAGTGCGTACCGGTGATTCGCT
ATGGGCCATCGCCCAGCGGTTCAATGTCTCATATAATGAACTGATGCGCTGGAACAACCTCGGCCGCAGAAGCATCATCC
GCCCCGGCGACAAACTGACTCTGTATGTGGACTGA

Upstream 100 bases:

>100_bases
CGTTTAGGGTAACAGGAGGCTACTTCACCCCGCAGTACCCGCGTTTTGCCGCGACTGCAAGGGTAATCAGAAACAAACCA
TAGGGAGACACCGGAACCTT

Downstream 100 bases:

>100_bases
TGCGGCTATAAAGCCTGACCCCGCAAGCCCCGCCACCCGGCGGGGCTTTTTGTGTTATGAGCTGATGATATTTATAGTGT
TTAAAAACAAGATGTTAGAA

Product: peptidoglycan-binding LysM

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MHIFSRGFAPGRAGVLALLVLMLAGGGCVSKNSPQPELPPVAEVQACPAPLQEQDASISDAEEVVEIEVPPADDNQPLTV
EEEQALLTQLDIDIEMDESDRSVVESYIKYFTHRARGSFERYLERAEVYLPAAREVFRQKGLPEELVYLAFVESGFNPNA
YSRAGAAGVWQFMPFTGRKYGLRYDWWIDERRDPYKSAEAAANYLSWLYAEFGDWYLALAAYNAGEGKIGRAIAGTGAES
FFELTSQNHKLSHRKQLRRETRHYVPKFIAIVKIMRNLEELGFKPLRLDCAPELNPLQVKGGTDLLALADAAGMKWDDFK
KYNTAFLRYVTPPDAETTVYVTPAAKSGALAFLAKPESRPYAGWADYKVRSGDSWYRISRRYGVPVSVLKKVNQKKSNLL
RPGERLMIPRTGSGRIPSSPMQETRALAQKRGDYVVSQGDTLYDIARNYGLTVATLKQANGLRSSMLRPGQKLYIPGQTR
AQEARTRKDAEQARKAVVYRVRTGDSLWAIAQRFNVSYNELMRWNNLGRRSIIRPGDKLTLYVD

Sequences:

>Translated_544_residues
MHIFSRGFAPGRAGVLALLVLMLAGGGCVSKNSPQPELPPVAEVQACPAPLQEQDASISDAEEVVEIEVPPADDNQPLTV
EEEQALLTQLDIDIEMDESDRSVVESYIKYFTHRARGSFERYLERAEVYLPAAREVFRQKGLPEELVYLAFVESGFNPNA
YSRAGAAGVWQFMPFTGRKYGLRYDWWIDERRDPYKSAEAAANYLSWLYAEFGDWYLALAAYNAGEGKIGRAIAGTGAES
FFELTSQNHKLSHRKQLRRETRHYVPKFIAIVKIMRNLEELGFKPLRLDCAPELNPLQVKGGTDLLALADAAGMKWDDFK
KYNTAFLRYVTPPDAETTVYVTPAAKSGALAFLAKPESRPYAGWADYKVRSGDSWYRISRRYGVPVSVLKKVNQKKSNLL
RPGERLMIPRTGSGRIPSSPMQETRALAQKRGDYVVSQGDTLYDIARNYGLTVATLKQANGLRSSMLRPGQKLYIPGQTR
AQEARTRKDAEQARKAVVYRVRTGDSLWAIAQRFNVSYNELMRWNNLGRRSIIRPGDKLTLYVD
>Mature_544_residues
MHIFSRGFAPGRAGVLALLVLMLAGGGCVSKNSPQPELPPVAEVQACPAPLQEQDASISDAEEVVEIEVPPADDNQPLTV
EEEQALLTQLDIDIEMDESDRSVVESYIKYFTHRARGSFERYLERAEVYLPAAREVFRQKGLPEELVYLAFVESGFNPNA
YSRAGAAGVWQFMPFTGRKYGLRYDWWIDERRDPYKSAEAAANYLSWLYAEFGDWYLALAAYNAGEGKIGRAIAGTGAES
FFELTSQNHKLSHRKQLRRETRHYVPKFIAIVKIMRNLEELGFKPLRLDCAPELNPLQVKGGTDLLALADAAGMKWDDFK
KYNTAFLRYVTPPDAETTVYVTPAAKSGALAFLAKPESRPYAGWADYKVRSGDSWYRISRRYGVPVSVLKKVNQKKSNLL
RPGERLMIPRTGSGRIPSSPMQETRALAQKRGDYVVSQGDTLYDIARNYGLTVATLKQANGLRSSMLRPGQKLYIPGQTR
AQEARTRKDAEQARKAVVYRVRTGDSLWAIAQRFNVSYNELMRWNNLGRRSIIRPGDKLTLYVD

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=402, Percent_Identity=30.0995024875622, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 61247; Mature: 61247

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIFSRGFAPGRAGVLALLVLMLAGGGCVSKNSPQPELPPVAEVQACPAPLQEQDASISD
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHCCCCCCC
AEEVVEIEVPPADDNQPLTVEEEQALLTQLDIDIEMDESDRSVVESYIKYFTHRARGSFE
CCCEEEEECCCCCCCCCEEECHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHH
RYLERAEVYLPAAREVFRQKGLPEELVYLAFVESGFNPNAYSRAGAAGVWQFMPFTGRKY
HHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCC
GLRYDWWIDERRDPYKSAEAAANYLSWLYAEFGDWYLALAAYNAGEGKIGRAIAGTGAES
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEECCCHHH
FFELTSQNHKLSHRKQLRRETRHYVPKFIAIVKIMRNLEELGFKPLRLDCAPELNPLQVK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEC
GGTDLLALADAAGMKWDDFKKYNTAFLRYVTPPDAETTVYVTPAAKSGALAFLAKPESRP
CCCCEEEEHHHCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCC
YAGWADYKVRSGDSWYRISRRYGVPVSVLKKVNQKKSNLLRPGERLMIPRTGSGRIPSSP
CCCCCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCH
MQETRALAQKRGDYVVSQGDTLYDIARNYGLTVATLKQANGLRSSMLRPGQKLYIPGQTR
HHHHHHHHHHCCCEEEECCCHHHHHHHHCCCEEEEHHHHCCHHHHHCCCCCEEEECCCCH
AQEARTRKDAEQARKAVVYRVRTGDSLWAIAQRFNVSYNELMRWNNLGRRSIIRPGDKLT
HHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEE
LYVD
EEEC
>Mature Secondary Structure
MHIFSRGFAPGRAGVLALLVLMLAGGGCVSKNSPQPELPPVAEVQACPAPLQEQDASISD
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHCCCCCCC
AEEVVEIEVPPADDNQPLTVEEEQALLTQLDIDIEMDESDRSVVESYIKYFTHRARGSFE
CCCEEEEECCCCCCCCCEEECHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHH
RYLERAEVYLPAAREVFRQKGLPEELVYLAFVESGFNPNAYSRAGAAGVWQFMPFTGRKY
HHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCCCCCCC
GLRYDWWIDERRDPYKSAEAAANYLSWLYAEFGDWYLALAAYNAGEGKIGRAIAGTGAES
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCEEECCCHHH
FFELTSQNHKLSHRKQLRRETRHYVPKFIAIVKIMRNLEELGFKPLRLDCAPELNPLQVK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEC
GGTDLLALADAAGMKWDDFKKYNTAFLRYVTPPDAETTVYVTPAAKSGALAFLAKPESRP
CCCCEEEEHHHCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCC
YAGWADYKVRSGDSWYRISRRYGVPVSVLKKVNQKKSNLLRPGERLMIPRTGSGRIPSSP
CCCCCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCH
MQETRALAQKRGDYVVSQGDTLYDIARNYGLTVATLKQANGLRSSMLRPGQKLYIPGQTR
HHHHHHHHHHCCCEEEECCCHHHHHHHHCCCEEEEHHHHCCHHHHHCCCCCEEEECCCCH
AQEARTRKDAEQARKAVVYRVRTGDSLWAIAQRFNVSYNELMRWNNLGRRSIIRPGDKLT
HHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEE
LYVD
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]