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The map label for this gene is asnB [H]

Identifier: 78358357

GI number: 78358357

Start: 3302685

End: 3304574

Strand: Reverse

Name: asnB [H]

Synonym: Dde_3317

Alternate gene names: 78358357

Gene position: 3304574-3302685 (Counterclockwise)

Preceding gene: 78358358

Following gene: 78358356

Centisome position: 88.59

GC content: 60.05

Gene sequence:

>1890_bases
ATGTGCGGAATAGCAGGATATTGCGGCGCCTGTGGCGTTGCTGACACCACAGAAAAGGAATGTGCCGGACGTCTTGAGGC
GCTGCTGGCCGGTATTGTCCATCGCGGTCCCGATGATGCGGGCATTACCTGTGCGCATAATGTGGCCATAGGCATGCGGC
GGCTTTCCATCATCGACCTTGCTGGCGGGCATCAGCCCATGTTGTCCGGAGACGGCCGCTATGCGCTGGTGTTCAACGGC
GAAATATACAACTTCAGAGAACTGCGCAGCGAACTGGCGGAGGCGGGATACTCATTCGCCACGAACAGTGACAGCGAAGT
ACTGCTCAAGGGGTATATGGCGTGGGGCAGGGGGGTTCTTGACCGGCTTGACGGCATGTTCGCCTTTGCCGTGCATGACA
GACATGCCGGTACAGTGTTTCTGGCGCGTGATCATCTGGGTATCAAGCCGCTGTATTACATCCATTTGGGCGGGGTGTTT
GCTTTCTGTTCCGAACCTGCTCCGCTTCTGGAACTGCCGCAGGTCCGCCGCAGGCTTGACCACGACGCGCTGGCAGCGTT
TCTGAGCTTTAAGTATGTGCCCGCACCGGATACGTTTATCAAGGATATCAAAAAGCTCGCAGCAGGCCACACGCTTGAGT
ATTCGCCGCGCGGCGACATGTTGCGCACGGAGCGTTACTGGCAGCTGCAGGGCCGCGAAGGCAGCAGTGATGCGGGCGAA
CTGGCTGAAAGACTGACCCGTGCGGTTGACAGCCAGATGGTCAGCGATGTGCCGCTGGGGGTTTTTCTGAGCGGCGGCAT
AGACAGCGGGCTGCTGACATGGGCCGCCGCAGGGCAGGGCGGAGAGCATTTCACGGGCGGCTATACCGTAGCCTTTGACG
AGACAGGCCGTAAAAAAGGCCTGCGCGCGGGCGGTTCATTTGATGAAAGCCGTCTGGCCGCCCTGAGTGCCGCGCATCTG
GGTGTGCCTCTTTCCAGCACTACGCTTGCAAGTCCGGCACAGGCGACACTGGATGACTGGATACGGCGGTTTCAGGAGCC
GTTTGCCAATACCAGCATACCGGCCAATTTTTTGCTGTGCCAGTGGGTTGCCCGGCACGTCAAAGTCGCCCTGAACGGTT
CAGGTGCCGATGAGCTTTTCGGCGGGTATGACAGGTACTACGCCGTGCATCCTCCGGCAGCCCTGCGCGCCCTGCACTGT
GCGGCGCCGCTGCTGCGGCGTGTGGCGGACATGCTGCCGGTAGGGGGCGGAAAGAACTCTCTGGTGCACCGTGCCAGACG
CTATCTGGCAGGCTGTGCACTTGCCCCCGCGCAAAAACATGCCGGCAGCATCAGACTGTTTACCGATACCCAGCTTGCAG
CCATTGCTCCCGGTGTTGTGCCGGCGACTTTTGTACAGGATGCTTTTGCCGGTGCGCCTCAGGGCAGCCTGCTGTACAAG
GCCACGTGGACAGATATGCAGACCATGCTGCCGGACGACTATCTGACTCTGGTGGACAGAACCAGCATGGCGGCTTCGCT
GGAAGTACGTGTACCGTTTCTTGCCAGGGATTTTGTGCAGTACGCCTTTTCATTGCCGCCGGCCGCAAAGATTGCGGGGT
GGAAAAAGAAAACCGCCCTGCGTGCGCTTGCCGCAAAGCACCTGCCCGAAGACATTGCACGCCAGCCTAAAATGGGGTTT
GAATCGCCGGTGGGCCGGTGGTTCCGTGACGGGCTGGGTGAGGCACTGTGCCGGCAGCTGGCGCAAAGCCCGCTGCAGAA
TATTATATCGGTACGGGGGGTGCAGGCGCTGGCACAAAGCCACAGCGCCGGTCAGGTGGACGCAGCCAAGCCGCTTCTGG
CGTTGTATACCCTTGTGCGCTGGATGGATATGTACAGGGTGGAACTTTAG

Upstream 100 bases:

>100_bases
TGCTCTGCCATGCGTTGTATCTTCTCTTTTTCGGCAATGACCTTTTTTCTTCGGGCAACAAGCTGTTCATGCTGTGCCGC
GGCAGTAAAGCGTAACGGCC

Downstream 100 bases:

>100_bases
CCATGCAGATCAGCTTCATATCGCACGAATATCCCCCTGTGAGCGGCGGCGGGTCCACGGCCCTGCACGAGTATGCCTCG
GGGCTTGCCACCCGCGGCCA

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MCGIAGYCGACGVADTTEKECAGRLEALLAGIVHRGPDDAGITCAHNVAIGMRRLSIIDLAGGHQPMLSGDGRYALVFNG
EIYNFRELRSELAEAGYSFATNSDSEVLLKGYMAWGRGVLDRLDGMFAFAVHDRHAGTVFLARDHLGIKPLYYIHLGGVF
AFCSEPAPLLELPQVRRRLDHDALAAFLSFKYVPAPDTFIKDIKKLAAGHTLEYSPRGDMLRTERYWQLQGREGSSDAGE
LAERLTRAVDSQMVSDVPLGVFLSGGIDSGLLTWAAAGQGGEHFTGGYTVAFDETGRKKGLRAGGSFDESRLAALSAAHL
GVPLSSTTLASPAQATLDDWIRRFQEPFANTSIPANFLLCQWVARHVKVALNGSGADELFGGYDRYYAVHPPAALRALHC
AAPLLRRVADMLPVGGGKNSLVHRARRYLAGCALAPAQKHAGSIRLFTDTQLAAIAPGVVPATFVQDAFAGAPQGSLLYK
ATWTDMQTMLPDDYLTLVDRTSMAASLEVRVPFLARDFVQYAFSLPPAAKIAGWKKKTALRALAAKHLPEDIARQPKMGF
ESPVGRWFRDGLGEALCRQLAQSPLQNIISVRGVQALAQSHSAGQVDAAKPLLALYTLVRWMDMYRVEL

Sequences:

>Translated_629_residues
MCGIAGYCGACGVADTTEKECAGRLEALLAGIVHRGPDDAGITCAHNVAIGMRRLSIIDLAGGHQPMLSGDGRYALVFNG
EIYNFRELRSELAEAGYSFATNSDSEVLLKGYMAWGRGVLDRLDGMFAFAVHDRHAGTVFLARDHLGIKPLYYIHLGGVF
AFCSEPAPLLELPQVRRRLDHDALAAFLSFKYVPAPDTFIKDIKKLAAGHTLEYSPRGDMLRTERYWQLQGREGSSDAGE
LAERLTRAVDSQMVSDVPLGVFLSGGIDSGLLTWAAAGQGGEHFTGGYTVAFDETGRKKGLRAGGSFDESRLAALSAAHL
GVPLSSTTLASPAQATLDDWIRRFQEPFANTSIPANFLLCQWVARHVKVALNGSGADELFGGYDRYYAVHPPAALRALHC
AAPLLRRVADMLPVGGGKNSLVHRARRYLAGCALAPAQKHAGSIRLFTDTQLAAIAPGVVPATFVQDAFAGAPQGSLLYK
ATWTDMQTMLPDDYLTLVDRTSMAASLEVRVPFLARDFVQYAFSLPPAAKIAGWKKKTALRALAAKHLPEDIARQPKMGF
ESPVGRWFRDGLGEALCRQLAQSPLQNIISVRGVQALAQSHSAGQVDAAKPLLALYTLVRWMDMYRVEL
>Mature_629_residues
MCGIAGYCGACGVADTTEKECAGRLEALLAGIVHRGPDDAGITCAHNVAIGMRRLSIIDLAGGHQPMLSGDGRYALVFNG
EIYNFRELRSELAEAGYSFATNSDSEVLLKGYMAWGRGVLDRLDGMFAFAVHDRHAGTVFLARDHLGIKPLYYIHLGGVF
AFCSEPAPLLELPQVRRRLDHDALAAFLSFKYVPAPDTFIKDIKKLAAGHTLEYSPRGDMLRTERYWQLQGREGSSDAGE
LAERLTRAVDSQMVSDVPLGVFLSGGIDSGLLTWAAAGQGGEHFTGGYTVAFDETGRKKGLRAGGSFDESRLAALSAAHL
GVPLSSTTLASPAQATLDDWIRRFQEPFANTSIPANFLLCQWVARHVKVALNGSGADELFGGYDRYYAVHPPAALRALHC
AAPLLRRVADMLPVGGGKNSLVHRARRYLAGCALAPAQKHAGSIRLFTDTQLAAIAPGVVPATFVQDAFAGAPQGSLLYK
ATWTDMQTMLPDDYLTLVDRTSMAASLEVRVPFLARDFVQYAFSLPPAAKIAGWKKKTALRALAAKHLPEDIARQPKMGF
ESPVGRWFRDGLGEALCRQLAQSPLQNIISVRGVQALAQSHSAGQVDAAKPLLALYTLVRWMDMYRVEL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=412, Percent_Identity=25.9708737864078, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI168229252, Length=412, Percent_Identity=25.9708737864078, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI168229250, Length=412, Percent_Identity=25.9708737864078, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI296010848, Length=381, Percent_Identity=26.246719160105, Blast_Score=90, Evalue=7e-18,
Organism=Escherichia coli, GI1786889, Length=391, Percent_Identity=32.4808184143223, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71993933, Length=407, Percent_Identity=27.5184275184275, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25147557, Length=407, Percent_Identity=27.5184275184275, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25147560, Length=285, Percent_Identity=29.1228070175439, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325403, Length=396, Percent_Identity=30.5555555555556, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=392, Percent_Identity=29.3367346938776, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45553209, Length=362, Percent_Identity=30.939226519337, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68047; Mature: 68047

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00435 PEROXIDASE_1 ; PS00605 ATPASE_C

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGYCGACGVADTTEKECAGRLEALLAGIVHRGPDDAGITCAHNVAIGMRRLSIIDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEEEE
AGGHQPMLSGDGRYALVFNGEIYNFRELRSELAEAGYSFATNSDSEVLLKGYMAWGRGVL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
DRLDGMFAFAVHDRHAGTVFLARDHLGIKPLYYIHLGGVFAFCSEPAPLLELPQVRRRLD
HHHCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEECHHHHHHCCCCCHHHHHHHHHHHC
HDALAAFLSFKYVPAPDTFIKDIKKLAAGHTLEYSPRGDMLRTERYWQLQGREGSSDAGE
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEECCCEEEEECCCCCCCHHH
LAERLTRAVDSQMVSDVPLGVFLSGGIDSGLLTWAAAGQGGEHFTGGYTVAFDETGRKKG
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEECCCCCCCCCCCEEEEECCCCCCCC
LRAGGSFDESRLAALSAAHLGVPLSSTTLASPAQATLDDWIRRFQEPFANTSIPANFLLC
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
QWVARHVKVALNGSGADELFGGYDRYYAVHPPAALRALHCAAPLLRRVADMLPVGGGKNS
HHHHHHHEEEECCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LVHRARRYLAGCALAPAQKHAGSIRLFTDTQLAAIAPGVVPATFVQDAFAGAPQGSLLYK
HHHHHHHHHHHCCCCCHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
ATWTDMQTMLPDDYLTLVDRTSMAASLEVRVPFLARDFVQYAFSLPPAAKIAGWKKKTAL
EEHHHHHHHCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCHHHHCCCHHHHHH
RALAAKHLPEDIARQPKMGFESPVGRWFRDGLGEALCRQLAQSPLQNIISVRGVQALAQS
HHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HSAGQVDAAKPLLALYTLVRWMDMYRVEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGYCGACGVADTTEKECAGRLEALLAGIVHRGPDDAGITCAHNVAIGMRRLSIIDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEEEE
AGGHQPMLSGDGRYALVFNGEIYNFRELRSELAEAGYSFATNSDSEVLLKGYMAWGRGVL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
DRLDGMFAFAVHDRHAGTVFLARDHLGIKPLYYIHLGGVFAFCSEPAPLLELPQVRRRLD
HHHCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEECHHHHHHCCCCCHHHHHHHHHHHC
HDALAAFLSFKYVPAPDTFIKDIKKLAAGHTLEYSPRGDMLRTERYWQLQGREGSSDAGE
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEECCCEEEEECCCCCCCHHH
LAERLTRAVDSQMVSDVPLGVFLSGGIDSGLLTWAAAGQGGEHFTGGYTVAFDETGRKKG
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEECCCCCCCCCCCEEEEECCCCCCCC
LRAGGSFDESRLAALSAAHLGVPLSSTTLASPAQATLDDWIRRFQEPFANTSIPANFLLC
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
QWVARHVKVALNGSGADELFGGYDRYYAVHPPAALRALHCAAPLLRRVADMLPVGGGKNS
HHHHHHHEEEECCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LVHRARRYLAGCALAPAQKHAGSIRLFTDTQLAAIAPGVVPATFVQDAFAGAPQGSLLYK
HHHHHHHHHHHCCCCCHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEE
ATWTDMQTMLPDDYLTLVDRTSMAASLEVRVPFLARDFVQYAFSLPPAAKIAGWKKKTAL
EEHHHHHHHCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCHHHHCCCHHHHHH
RALAAKHLPEDIARQPKMGFESPVGRWFRDGLGEALCRQLAQSPLQNIISVRGVQALAQS
HHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HSAGQVDAAKPLLALYTLVRWMDMYRVEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]