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The map label for this gene is pspA [H]

Identifier: 78358266

GI number: 78358266

Start: 3214999

End: 3215664

Strand: Reverse

Name: pspA [H]

Synonym: Dde_3226

Alternate gene names: 78358266

Gene position: 3215664-3214999 (Counterclockwise)

Preceding gene: 78358270

Following gene: 78358265

Centisome position: 86.21

GC content: 60.06

Gene sequence:

>666_bases
ATGGGTATTTTTTCCCGGTTCAAGGATATCGTGAGTGCCAACATGAGCGCCATGCTGGACAAGGCAGAAGATCCTGAAAA
ACTGATCAGGCTTATGATTCGCGAGATGGAAGAAACGCTGGTCGAGCTGAAGGCCAACTGCGCCGGTACGATGGCTGAAG
CCACCCGTGTCGGACGCGAGCTTGACGTGGTGGAAAAGGCCAGAGAGCGCTGGGCACAGCGGGCTGAACTGGCTGTCAGC
CGCGGGCGCGAAGATCTGGCGCGCGAAGCGCTGCTGGAAAAACGGCGCGAACAGGAACGTGCGGATGCGTTGCTGCGCGA
GATGGCCGGTTGTGAGGCTCTGGTGGCCGAGGCGCGCAACGATATAGCCACGCTGGAAGAAAAGCTGGCATCGGCACGTG
AAAAGCAGCGTCTGCTCATGCAGCGCCATGCGCATGCCAGAGTGCGTACGCAGGCGCGGCGTGATGTGAACCGCGCTGTT
TCGCTGGACGCCATGCGCCGTTTTGACGAGCTGGAACAGCGGGTCGAGTTTATGGAAGCCGAAGCCGAGATTGAAGTCCC
GCAGCGCAAGCCCGACCTTGAGGACCGGTTTGCCCAGCTGGAAGGTGCGGATGAAATAGAGCGTGAACTGGATGCCCTGC
GCCGCAAGGTGAATCCTTCCGCGTAA

Upstream 100 bases:

>100_bases
GCCCGATATAAAATAAACTGTAAAAACAGAATGATGTGTCAGTTGAGCGCCTCTGGCATAGAAGGTGCTTATTTACTGCC
AACCAACCAAGGAGGATGCC

Downstream 100 bases:

>100_bases
AAAAAAACGGGCAGGGCGTGCGGGGGCAGGTGGTTGTATGCCGCGCCGTGCTGGGGTAGCATGGCTGGCCTGTATGTTGC
CACCCGCTGCCGCGGCTTTG

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MGIFSRFKDIVSANMSAMLDKAEDPEKLIRLMIREMEETLVELKANCAGTMAEATRVGRELDVVEKARERWAQRAELAVS
RGREDLAREALLEKRREQERADALLREMAGCEALVAEARNDIATLEEKLASAREKQRLLMQRHAHARVRTQARRDVNRAV
SLDAMRRFDELEQRVEFMEAEAEIEVPQRKPDLEDRFAQLEGADEIERELDALRRKVNPSA

Sequences:

>Translated_221_residues
MGIFSRFKDIVSANMSAMLDKAEDPEKLIRLMIREMEETLVELKANCAGTMAEATRVGRELDVVEKARERWAQRAELAVS
RGREDLAREALLEKRREQERADALLREMAGCEALVAEARNDIATLEEKLASAREKQRLLMQRHAHARVRTQARRDVNRAV
SLDAMRRFDELEQRVEFMEAEAEIEVPQRKPDLEDRFAQLEGADEIERELDALRRKVNPSA
>Mature_220_residues
GIFSRFKDIVSANMSAMLDKAEDPEKLIRLMIREMEETLVELKANCAGTMAEATRVGRELDVVEKARERWAQRAELAVSR
GREDLAREALLEKRREQERADALLREMAGCEALVAEARNDIATLEEKLASAREKQRLLMQRHAHARVRTQARRDVNRAVS
LDAMRRFDELEQRVEFMEAEAEIEVPQRKPDLEDRFAQLEGADEIERELDALRRKVNPSA

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=217, Percent_Identity=41.4746543778802, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25484; Mature: 25353

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFKDIVSANMSAMLDKAEDPEKLIRLMIREMEETLVELKANCAGTMAEATRVGRE
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDVVEKARERWAQRAELAVSRGREDLAREALLEKRREQERADALLREMAGCEALVAEARN
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIATLEEKLASAREKQRLLMQRHAHARVRTQARRDVNRAVSLDAMRRFDELEQRVEFMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEIEVPQRKPDLEDRFAQLEGADEIERELDALRRKVNPSA
HHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIFSRFKDIVSANMSAMLDKAEDPEKLIRLMIREMEETLVELKANCAGTMAEATRVGRE
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDVVEKARERWAQRAELAVSRGREDLAREALLEKRREQERADALLREMAGCEALVAEARN
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIATLEEKLASAREKQRLLMQRHAHARVRTQARRDVNRAVSLDAMRRFDELEQRVEFMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEIEVPQRKPDLEDRFAQLEGADEIERELDALRRKVNPSA
HHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]