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The map label for this gene is dosC [H]

Identifier: 78355914

GI number: 78355914

Start: 895487

End: 896425

Strand: Direct

Name: dosC [H]

Synonym: Dde_0867

Alternate gene names: 78355914

Gene position: 895487-896425 (Clockwise)

Preceding gene: 78355913

Following gene: 78355919

Centisome position: 24.01

GC content: 48.56

Gene sequence:

>939_bases
ATGGAACGTTTTTACAGGGAATTATTAGATACACTTACCGACGGTATTTATTTTGTCACTTCCTCAGGTAAGATTACATA
CTGGAATAAATCTGCAGAGCGATTGAGTGGCTATGCTGCCAGCGAAGTAGTGGGAAAGAGCTGCGGTGATAATGTGCTGC
GACACGTGGATGACTGCGGGACTCCTCTGTGTCTTACCGGATGCCCTCTGGCAGCCACTCTCAGAGATAGCAAGATTCGC
GAGGCCCGGGTGTATATGCATCATAAACAGGGATACAGGGTGCCGGTGCTCGTGCGTACTTTTCCTATGGCTGATGCACA
GGGAAACTCCATAGGCGCGGTGGAAGTTTTTACTGATGACAGCAAAAATATTAATTTTAAAAAAGAGATGGAAGAGCTCA
GGCGGGAAGTCCTGACCGATCCCCTGACAGAGGTTGGAAACCGTCGTTACGCAGAGATAATTCTTGAGCGCATGCAGGTC
AGTATGAATGATGTCGGTTCGCCGTATGCCGTTATCTTTGTTGATATTGATTTGTTTAAAGATGTTAATGATACATGGGG
GCACATTGTTGGTGACAATGTTTTGAAAATGGTTGCGAAAACCATTGAATCCTCTTTGCGGCCCCTTGATGCGGTTTGCA
GGTGGGGTGGCGAGGAATTTATAATCTGCGTGCCCGGTGCCACAAAAGACGGAGTTATGCAGCTTATGGAGCGGTTGCGG
ATGCTAGTTCAGGAATCGTGGATTATGCATGAACAGCATAAAATAGCGGTGACCGCTTCCTTCGGGGCTGCAATTTGCTC
ACAGGGAGAGTCTTCCGAGTCTGTGGCGCAACGGGCTGACGAGCAGCTTTATCTTAGCAAAAATACAGGACGGAACTGTG
TGCATCTTGACGGAGTGCGAGGTGAACAATGCTCCCGTCCCTCCTGCCGTTGTGCATGA

Upstream 100 bases:

>100_bases
TTTTGTTTCCGTCTGTATATTGAGTTTTGTAAAAAATATTATAATACTATTGTGATGTTTTTATTATTTCGCGTCGAATA
CGGAGAATAGAGGGGGCGTT

Downstream 100 bases:

>100_bases
ATAATGGTCTTTGTCTTGACGGCAGCGGCGATGGAGAGGCACAACGCAAAAGGCCCCGCATCTGTCTGCATGCGGGGCTT
TTCGTGTAACAGAAGAAGCG

Product: diguanylate cyclase with PAS/PAC sensor

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MERFYRELLDTLTDGIYFVTSSGKITYWNKSAERLSGYAASEVVGKSCGDNVLRHVDDCGTPLCLTGCPLAATLRDSKIR
EARVYMHHKQGYRVPVLVRTFPMADAQGNSIGAVEVFTDDSKNINFKKEMEELRREVLTDPLTEVGNRRYAEIILERMQV
SMNDVGSPYAVIFVDIDLFKDVNDTWGHIVGDNVLKMVAKTIESSLRPLDAVCRWGGEEFIICVPGATKDGVMQLMERLR
MLVQESWIMHEQHKIAVTASFGAAICSQGESSESVAQRADEQLYLSKNTGRNCVHLDGVRGEQCSRPSCRCA

Sequences:

>Translated_312_residues
MERFYRELLDTLTDGIYFVTSSGKITYWNKSAERLSGYAASEVVGKSCGDNVLRHVDDCGTPLCLTGCPLAATLRDSKIR
EARVYMHHKQGYRVPVLVRTFPMADAQGNSIGAVEVFTDDSKNINFKKEMEELRREVLTDPLTEVGNRRYAEIILERMQV
SMNDVGSPYAVIFVDIDLFKDVNDTWGHIVGDNVLKMVAKTIESSLRPLDAVCRWGGEEFIICVPGATKDGVMQLMERLR
MLVQESWIMHEQHKIAVTASFGAAICSQGESSESVAQRADEQLYLSKNTGRNCVHLDGVRGEQCSRPSCRCA
>Mature_312_residues
MERFYRELLDTLTDGIYFVTSSGKITYWNKSAERLSGYAASEVVGKSCGDNVLRHVDDCGTPLCLTGCPLAATLRDSKIR
EARVYMHHKQGYRVPVLVRTFPMADAQGNSIGAVEVFTDDSKNINFKKEMEELRREVLTDPLTEVGNRRYAEIILERMQV
SMNDVGSPYAVIFVDIDLFKDVNDTWGHIVGDNVLKMVAKTIESSLRPLDAVCRWGGEEFIICVPGATKDGVMQLMERLR
MLVQESWIMHEQHKIAVTASFGAAICSQGESSESVAQRADEQLYLSKNTGRNCVHLDGVRGEQCSRPSCRCA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=38.4146341463415, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081881, Length=189, Percent_Identity=34.9206349206349, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788381, Length=287, Percent_Identity=28.9198606271777, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=38.9937106918239, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=132, Percent_Identity=40.1515151515151, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=128, Percent_Identity=39.0625, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787541, Length=302, Percent_Identity=23.5099337748344, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788956, Length=158, Percent_Identity=30.379746835443, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=120, Percent_Identity=38.3333333333333, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=29.2993630573248, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786584, Length=154, Percent_Identity=29.2207792207792, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787802, Length=127, Percent_Identity=33.0708661417323, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=29.4444444444444, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34902; Mature: 34902

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFYRELLDTLTDGIYFVTSSGKITYWNKSAERLSGYAASEVVGKSCGDNVLRHVDDCG
CHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
TPLCLTGCPLAATLRDSKIREARVYMHHKQGYRVPVLVRTFPMADAQGNSIGAVEVFTDD
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEECC
SKNINFKKEMEELRREVLTDPLTEVGNRRYAEIILERMQVSMNDVGSPYAVIFVDIDLFK
CCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHH
DVNDTWGHIVGDNVLKMVAKTIESSLRPLDAVCRWGGEEFIICVPGATKDGVMQLMERLR
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
MLVQESWIMHEQHKIAVTASFGAAICSQGESSESVAQRADEQLYLSKNTGRNCVHLDGVR
HHHHHHHHHCCCCEEEEEECHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCEEEECCCC
GEQCSRPSCRCA
CCCCCCCCCCCC
>Mature Secondary Structure
MERFYRELLDTLTDGIYFVTSSGKITYWNKSAERLSGYAASEVVGKSCGDNVLRHVDDCG
CHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
TPLCLTGCPLAATLRDSKIREARVYMHHKQGYRVPVLVRTFPMADAQGNSIGAVEVFTDD
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEECC
SKNINFKKEMEELRREVLTDPLTEVGNRRYAEIILERMQVSMNDVGSPYAVIFVDIDLFK
CCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHH
DVNDTWGHIVGDNVLKMVAKTIESSLRPLDAVCRWGGEEFIICVPGATKDGVMQLMERLR
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
MLVQESWIMHEQHKIAVTASFGAAICSQGESSESVAQRADEQLYLSKNTGRNCVHLDGVR
HHHHHHHHHCCCCEEEEEECHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCEEEECCCC
GEQCSRPSCRCA
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]