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The map label for this gene is rimO

Identifier: 78355553

GI number: 78355553

Start: 513506

End: 514798

Strand: Direct

Name: rimO

Synonym: Dde_0506

Alternate gene names: 78355553

Gene position: 513506-514798 (Clockwise)

Preceding gene: 78355545

Following gene: 78355554

Centisome position: 13.77

GC content: 61.95

Gene sequence:

>1293_bases
ATGATAAGCGTTTATTCGATAAGTCTCGGCTGTCCCAAGAACCGCGTGGACACCGAGTGGCTGCTGGGCGTGCTGGGGCC
GGAGGTCCGACCCGTCCGGGAGATGGCGGATGCCGACCTTGTACTCATCAATACCTGCGGGTTCATTGCTCCGGCGGTGG
AAGAGTCTGTCCGGACGGTGGTCGAGGCCGTGGCCGAACTGGAAGACCTGCCCCGCAGGCCGCTGCTGGCTGTGGCCGGC
TGTCTGGTGGGCCGGTACGGCCGGCAGGACCTGGCCGCAGAACTGCCCGAGGTTGATCTGTGGCTGACCAACAGAGATAT
GGATGCATGGCCGGAGATGATAGGCCGCGCGCTGGGAGTTGCGGTGCATGTGCCGCCGGTGCGTCTGCTTTCCACCGGTC
CGTCATATGCCTACCTCAAGGTCAGCGACGGCTGCGGCCATAACTGTTCTTTCTGCACCATACCTTCCATCCGCGGCGGG
CTGGTGTCCACTCCGGCCGACGTGCTGGAAGCGGAGGCCGTCAACCTGCTTTCCCGCGGGGTGAAGGAGCTGATATTTGT
GGCGCAGGATGTTGCCGCCTACGGCAGAGACATGGGACTCAGGCACGGTCTGCGTTCGCTGCTGGACAGGCTGCTGCCGC
TGGACGGGCTGGAACGGCTGCGGCTCATGTATCTGTATCCTGCCGGTCTTGATGCCGGTCTGCTCCGTTATCTGCGTGAT
GCGGGCAAACCTTTTGTGCCGTATTTTGATATTCCCGTGCAGCACGCCCATCCTGATGTGCTGTCGCGCATGGGACGTCC
TTTTGCCCGCAACCCGCGCGAGGTGGTGGACCGCGTGCGCGATGTCTTTCCCGAAGCGGCGCTGCGCACGTCCATCATAG
TGGGCTTTCCCGGTGAGACACAGCAGCACTACGACCATCTGACGCGCTTTGTGCAGGATGTGCGTTTTATGCATCTGGGC
GTGTTTGCCTATCGTGCCGAAGAAGGCACCCCCGCAGCCGGCATGCCCGGTCAGGTGGATGAAGTGGAAAAAGACTGGCG
CCGCGATGCCCTGATGGAAGTGCAGGCAGAGATAAGCGAAGAAATTCTGGAAGGGTTTACCGGCAGCGACGAAGACGTGC
TGGTGGACGCCGCGCACGAGGAGTGGCCGGGGCTGCATGTGGGCCGCACATGGTTTCAGGCACCGGAAATTGACGGCGTG
ACCTACATAAGCGGACCGGGGGTGAAACCCGGAGCCATGGTGCGTGCCGAGATAGTGGAATCGCGGACCTATGATCTTGT
GGCACTCTCATAA

Upstream 100 bases:

>100_bases
TACGCGGTCCCGTCCGGCCGGCATTATTTTACTTGCATAGTGAGTCCCGCCGTGGCAATGCGCGGGTTGCACAGTTACCT
TTTACCCTGTCTTACTCCTT

Downstream 100 bases:

>100_bases
ACAGGATTTACAGTTGAGTTCCTGCCGCCTTTTTGGTAATGAGGCAAATCCCGCAGGACGGTAGCGGGAAAATTTGTTGG
TTGGAGGAAACGTATGACAA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MISVYSISLGCPKNRVDTEWLLGVLGPEVRPVREMADADLVLINTCGFIAPAVEESVRTVVEAVAELEDLPRRPLLAVAG
CLVGRYGRQDLAAELPEVDLWLTNRDMDAWPEMIGRALGVAVHVPPVRLLSTGPSYAYLKVSDGCGHNCSFCTIPSIRGG
LVSTPADVLEAEAVNLLSRGVKELIFVAQDVAAYGRDMGLRHGLRSLLDRLLPLDGLERLRLMYLYPAGLDAGLLRYLRD
AGKPFVPYFDIPVQHAHPDVLSRMGRPFARNPREVVDRVRDVFPEAALRTSIIVGFPGETQQHYDHLTRFVQDVRFMHLG
VFAYRAEEGTPAAGMPGQVDEVEKDWRRDALMEVQAEISEEILEGFTGSDEDVLVDAAHEEWPGLHVGRTWFQAPEIDGV
TYISGPGVKPGAMVRAEIVESRTYDLVALS

Sequences:

>Translated_430_residues
MISVYSISLGCPKNRVDTEWLLGVLGPEVRPVREMADADLVLINTCGFIAPAVEESVRTVVEAVAELEDLPRRPLLAVAG
CLVGRYGRQDLAAELPEVDLWLTNRDMDAWPEMIGRALGVAVHVPPVRLLSTGPSYAYLKVSDGCGHNCSFCTIPSIRGG
LVSTPADVLEAEAVNLLSRGVKELIFVAQDVAAYGRDMGLRHGLRSLLDRLLPLDGLERLRLMYLYPAGLDAGLLRYLRD
AGKPFVPYFDIPVQHAHPDVLSRMGRPFARNPREVVDRVRDVFPEAALRTSIIVGFPGETQQHYDHLTRFVQDVRFMHLG
VFAYRAEEGTPAAGMPGQVDEVEKDWRRDALMEVQAEISEEILEGFTGSDEDVLVDAAHEEWPGLHVGRTWFQAPEIDGV
TYISGPGVKPGAMVRAEIVESRTYDLVALS
>Mature_430_residues
MISVYSISLGCPKNRVDTEWLLGVLGPEVRPVREMADADLVLINTCGFIAPAVEESVRTVVEAVAELEDLPRRPLLAVAG
CLVGRYGRQDLAAELPEVDLWLTNRDMDAWPEMIGRALGVAVHVPPVRLLSTGPSYAYLKVSDGCGHNCSFCTIPSIRGG
LVSTPADVLEAEAVNLLSRGVKELIFVAQDVAAYGRDMGLRHGLRSLLDRLLPLDGLERLRLMYLYPAGLDAGLLRYLRD
AGKPFVPYFDIPVQHAHPDVLSRMGRPFARNPREVVDRVRDVFPEAALRTSIIVGFPGETQQHYDHLTRFVQDVRFMHLG
VFAYRAEEGTPAAGMPGQVDEVEKDWRRDALMEVQAEISEEILEGFTGSDEDVLVDAAHEEWPGLHVGRTWFQAPEIDGV
TYISGPGVKPGAMVRAEIVESRTYDLVALS

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=361, Percent_Identity=29.9168975069252, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=37.020316027088, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1786882, Length=433, Percent_Identity=26.0969976905312, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71996771, Length=221, Percent_Identity=33.4841628959276, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922432, Length=354, Percent_Identity=26.271186440678, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=24.9422632794457, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_DESDG (Q315T9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387002.1
- ProteinModelPortal:   Q315T9
- SMR:   Q315T9
- STRING:   Q315T9
- GeneID:   3756529
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0506
- NMPDR:   fig|207559.3.peg.2046
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK706202
- BioCyc:   DDES207559:DDE_0506-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 47432; Mature: 47432

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVYSISLGCPKNRVDTEWLLGVLGPEVRPVREMADADLVLINTCGFIAPAVEESVRTV
CEEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
VEAVAELEDLPRRPLLAVAGCLVGRYGRQDLAAELPEVDLWLTNRDMDAWPEMIGRALGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCE
AVHVPPVRLLSTGPSYAYLKVSDGCGHNCSFCTIPSIRGGLVSTPADVLEAEAVNLLSRG
EEECCCCCEEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
VKELIFVAQDVAAYGRDMGLRHGLRSLLDRLLPLDGLERLRLMYLYPAGLDAGLLRYLRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCHHHHHHHHH
AGKPFVPYFDIPVQHAHPDVLSRMGRPFARNPREVVDRVRDVFPEAALRTSIIVGFPGET
CCCCCCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHCEEEEECCCCH
QQHYDHLTRFVQDVRFMHLGVFAYRAEEGTPAAGMPGQVDEVEKDWRRDALMEVQAEISE
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EILEGFTGSDEDVLVDAAHEEWPGLHVGRTWFQAPEIDGVTYISGPGVKPGAMVRAEIVE
HHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCHHHHHHHHH
SRTYDLVALS
CCCCEEEEEC
>Mature Secondary Structure
MISVYSISLGCPKNRVDTEWLLGVLGPEVRPVREMADADLVLINTCGFIAPAVEESVRTV
CEEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
VEAVAELEDLPRRPLLAVAGCLVGRYGRQDLAAELPEVDLWLTNRDMDAWPEMIGRALGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCE
AVHVPPVRLLSTGPSYAYLKVSDGCGHNCSFCTIPSIRGGLVSTPADVLEAEAVNLLSRG
EEECCCCCEEECCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
VKELIFVAQDVAAYGRDMGLRHGLRSLLDRLLPLDGLERLRLMYLYPAGLDAGLLRYLRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCHHHHHHHHH
AGKPFVPYFDIPVQHAHPDVLSRMGRPFARNPREVVDRVRDVFPEAALRTSIIVGFPGET
CCCCCCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHCEEEEECCCCH
QQHYDHLTRFVQDVRFMHLGVFAYRAEEGTPAAGMPGQVDEVEKDWRRDALMEVQAEISE
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EILEGFTGSDEDVLVDAAHEEWPGLHVGRTWFQAPEIDGVTYISGPGVKPGAMVRAEIVE
HHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCHHHHHHHHH
SRTYDLVALS
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA