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The map label for this gene is hydN [H]

Identifier: 78355523

GI number: 78355523

Start: 486400

End: 486894

Strand: Direct

Name: hydN [H]

Synonym: Dde_0476

Alternate gene names: 78355523

Gene position: 486400-486894 (Clockwise)

Preceding gene: 78355522

Following gene: 78355524

Centisome position: 13.04

GC content: 67.47

Gene sequence:

>495_bases
ATGAATGCTTTTATTGTGGCGGACCCGCGCCGGTGCATCGGGTGTGCCGCGTGCGAGGTGGCGTGTGCCGCGGCAGCGGC
AGGTCAGTCCGTGTTTGCCCCCGGGGGACAGGCAGCCGTATTCAGCCCCCGGCTGCATCTGGTGGCGGAAGCTGCGGTTA
CAGCGCCGGTGCAGTGCCGGCAATGTGCCGATGCTCCCTGTGCGGCAATTTGTCCGCGCGGAGCCATACGGATGGCGGAG
GGCGTGGTCACCGTGGACAGCGGGCTGTGCATCGGCTGCAAAATGTGCATGGTGGCGTGTCCGGTGGGGGCCATTGAGGT
GCGGGGCGGCAGGGCGTCCAAGTGTCAGCTGTGCACGGACCGTCCGGAAGGTCCGGCCTGTATGCAGGTGTGCCCTGCGG
AAGCATTTACGCTGGTGCGTGCGCAAACCCTGCGCGCCGAACTGGTACGCCGCAGAGCGGCCGGAGTATGCCCGGCTGCT
GATGCGCGGCTCTGA

Upstream 100 bases:

>100_bases
GTGCAGGCGTTGTACCGTGAATTTCTGGGCGAACCTCTGAGCCATGTTTCGCACAATCTGCTGCATACTGTTTACGGCCA
GACGCGTTAGGGAGGCTAAG

Downstream 100 bases:

>100_bases
CAGTAGGTCGGGCTGTAATGCCGGATGCTGTCTGATGTGCATTGAACGCCGTATCCTGTAAAAGGATACGGCGTTTTTTG
TATGCAGAAAACCCCCCGCT

Product: electron transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNAFIVADPRRCIGCAACEVACAAAAAGQSVFAPGGQAAVFSPRLHLVAEAAVTAPVQCRQCADAPCAAICPRGAIRMAE
GVVTVDSGLCIGCKMCMVACPVGAIEVRGGRASKCQLCTDRPEGPACMQVCPAEAFTLVRAQTLRAELVRRRAAGVCPAA
DARL

Sequences:

>Translated_164_residues
MNAFIVADPRRCIGCAACEVACAAAAAGQSVFAPGGQAAVFSPRLHLVAEAAVTAPVQCRQCADAPCAAICPRGAIRMAE
GVVTVDSGLCIGCKMCMVACPVGAIEVRGGRASKCQLCTDRPEGPACMQVCPAEAFTLVRAQTLRAELVRRRAAGVCPAA
DARL
>Mature_164_residues
MNAFIVADPRRCIGCAACEVACAAAAAGQSVFAPGGQAAVFSPRLHLVAEAAVTAPVQCRQCADAPCAAICPRGAIRMAE
GVVTVDSGLCIGCKMCMVACPVGAIEVRGGRASKCQLCTDRPEGPACMQVCPAEAFTLVRAQTLRAELVRRRAAGVCPAA
DARL

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=41.6184971098266, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=40.251572327044, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87082179, Length=151, Percent_Identity=41.7218543046358, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI87082180, Length=159, Percent_Identity=40.8805031446541, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1788811, Length=162, Percent_Identity=40.1234567901235, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI87082114, Length=191, Percent_Identity=31.9371727748691, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=33.5106382978723, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1787122, Length=155, Percent_Identity=31.6129032258064, Blast_Score=76, Evalue=9e-16,
Organism=Escherichia coli, GI1787872, Length=155, Percent_Identity=30.9677419354839, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI1789370, Length=180, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787963, Length=160, Percent_Identity=30, Blast_Score=63, Evalue=8e-12,
Organism=Escherichia coli, GI1790326, Length=164, Percent_Identity=28.6585365853659, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI2367345, Length=160, Percent_Identity=32.5, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787749, Length=170, Percent_Identity=28.2352941176471, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 16735; Mature: 16735

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
13.4 %Cys+Met (Translated Protein)
10.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
13.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFIVADPRRCIGCAACEVACAAAAAGQSVFAPGGQAAVFSPRLHLVAEAAVTAPVQCR
CCEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCHHHHHHHHHHCCCHHHH
QCADAPCAAICPRGAIRMAEGVVTVDSGLCIGCKMCMVACPVGAIEVRGGRASKCQLCTD
HHCCCCHHHHCCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCEECCC
RPEGPACMQVCPAEAFTLVRAQTLRAELVRRRAAGVCPAADARL
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAFIVADPRRCIGCAACEVACAAAAAGQSVFAPGGQAAVFSPRLHLVAEAAVTAPVQCR
CCEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCHHHHHHHHHHCCCHHHH
QCADAPCAAICPRGAIRMAEGVVTVDSGLCIGCKMCMVACPVGAIEVRGGRASKCQLCTD
HHCCCCHHHHCCCCCHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCEECCC
RPEGPACMQVCPAEAFTLVRAQTLRAELVRRRAAGVCPAADARL
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]