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The map label for this gene is purF [H]

Identifier: 78355381

GI number: 78355381

Start: 323124

End: 324512

Strand: Direct

Name: purF [H]

Synonym: Dde_0334

Alternate gene names: 78355381

Gene position: 323124-324512 (Clockwise)

Preceding gene: 78355380

Following gene: 78355382

Centisome position: 8.66

GC content: 55.72

Gene sequence:

>1389_bases
ATGAAACGTGAATACTGCGGCGTCTTCGGCATATACAACCATGCCGAAGCCGCCCGCATGACCTACTTCGGACTGTATGC
GCTGCAGCACCGCGGGCAGGAAAGTGCGGGCATTGTAACATGGGACGGTTCGGCCCTGCGTGAAGAACGCGGCATGGGGC
TGGTGCCGGAAGTGTTCAATGAACGGCATCTGAGCAAAGAACTCAAAGGCGATATCGCCATCGGCCACACCCGCTATTCC
ACCACCGGCGCATCGCTGCTGCGCAATGCCCAGCCGTTCCGTGTGCGCTATAAAAACATGGATATAGCCATCGCCCACAA
CGGTAATCTGGTAAACACCGTGGAGCTTCGTTCCAGACTGGAAGAAAGCGGCTCCATTTTCCAGACGACCAACGACAGCG
AGGTAATTGTCCATCTGATTGCCAAGCACATGAACGGAGGCACTGTCGAAGAAGCCGTCATGGCTGCCTGCCGCGAGGCA
CGCGGTGCGTATTCGCTGCTTATTCTGGCCAATGATAAGATGATAGCCGTGCGTGACCCGCACGGCTTTCGTCCTTTGCT
GCTGGGAAAAGTGGGCGACGCGCATGTCTTTGCCAGTGAATCGTGCGCCTTTGATCTGCTGGAGGCGGAGCTTATCCGTT
CCGTCAAGCCGGGCGAGATGATTGTCATCGATAAAGGCAAGGTGCACAGCTATGATATGGATATGGGCAAAAAGCGCCAC
CACTGTATCTTCGAGCTGATCTACTTTGCCCGCCCCGATTCCATGGTATTCGGAGAAAATGTCTACTTCTGCCGCAAAGC
CATGGGCAAGCAGCTGGCCACGGAAGCGCCGGTGGATGCCGACTTCGTCATGCCCTTTCCCGATTCCGGCGTATACTGCG
CCGTGGGATACGCCCAGCAGTCGGGGTTGCCGTACGAGCATGCCATGATCCGCAACCACTATGTGGGCCGCACCTTTATC
CAGCCTTCGCAGGATATGCGTGATTACAGCGTAAGGGTGAAGATCAACCCTGTGAAGGATATGATCAAGGACAAGCGCAT
CATCATTGTGGATGATTCCATCGTGCGGGGTACCACCATCCGCACCCGCGTGAAAAAACTGAGGGAGCTGGGCGCCCGCG
AAGTGCATTTCCGCGTGAGCTGCCCCCCCATCAAGTTTCCGTGTTTCTACGGCATTGATTTTGCCTCCAAGGGTGAACTG
ATTGCGGCCAACCATTCCGTAAGAGAGATAGAACGCTTTATCGGTCTGGACAGCCTGCATTACATCAGCATAGAGGGGCT
GCTCAAGTCGGTCAGTACGCCGGATGACTATTGTCTGGCATGCTTTACCGGCAGCTACCCGTTGCAGTGCGAAGGAAATC
TGAAGTCGTGCCTTGAGTGCGGCTGCTAA

Upstream 100 bases:

>100_bases
GCCATTGCCGAGCAGCGCCGCTGCGGGCTTACCGTGAAAAGCCTGCAGGAATACTACGCGGGCAGCACCGAAGCGCCCGT
ATGCGGGGAAAAATAATATC

Downstream 100 bases:

>100_bases
ATACGACATATTGTGCATGCGGGCTGCCGTGCCGGACACGGTGTGCCCGCATGCGCTTTTTTTATCGCAGCGTCATCCGG
TGTGTATCCGACAGCCCTGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKREYCGVFGIYNHAEAARMTYFGLYALQHRGQESAGIVTWDGSALREERGMGLVPEVFNERHLSKELKGDIAIGHTRYS
TTGASLLRNAQPFRVRYKNMDIAIAHNGNLVNTVELRSRLEESGSIFQTTNDSEVIVHLIAKHMNGGTVEEAVMAACREA
RGAYSLLILANDKMIAVRDPHGFRPLLLGKVGDAHVFASESCAFDLLEAELIRSVKPGEMIVIDKGKVHSYDMDMGKKRH
HCIFELIYFARPDSMVFGENVYFCRKAMGKQLATEAPVDADFVMPFPDSGVYCAVGYAQQSGLPYEHAMIRNHYVGRTFI
QPSQDMRDYSVRVKINPVKDMIKDKRIIIVDDSIVRGTTIRTRVKKLRELGAREVHFRVSCPPIKFPCFYGIDFASKGEL
IAANHSVREIERFIGLDSLHYISIEGLLKSVSTPDDYCLACFTGSYPLQCEGNLKSCLECGC

Sequences:

>Translated_462_residues
MKREYCGVFGIYNHAEAARMTYFGLYALQHRGQESAGIVTWDGSALREERGMGLVPEVFNERHLSKELKGDIAIGHTRYS
TTGASLLRNAQPFRVRYKNMDIAIAHNGNLVNTVELRSRLEESGSIFQTTNDSEVIVHLIAKHMNGGTVEEAVMAACREA
RGAYSLLILANDKMIAVRDPHGFRPLLLGKVGDAHVFASESCAFDLLEAELIRSVKPGEMIVIDKGKVHSYDMDMGKKRH
HCIFELIYFARPDSMVFGENVYFCRKAMGKQLATEAPVDADFVMPFPDSGVYCAVGYAQQSGLPYEHAMIRNHYVGRTFI
QPSQDMRDYSVRVKINPVKDMIKDKRIIIVDDSIVRGTTIRTRVKKLRELGAREVHFRVSCPPIKFPCFYGIDFASKGEL
IAANHSVREIERFIGLDSLHYISIEGLLKSVSTPDDYCLACFTGSYPLQCEGNLKSCLECGC
>Mature_462_residues
MKREYCGVFGIYNHAEAARMTYFGLYALQHRGQESAGIVTWDGSALREERGMGLVPEVFNERHLSKELKGDIAIGHTRYS
TTGASLLRNAQPFRVRYKNMDIAIAHNGNLVNTVELRSRLEESGSIFQTTNDSEVIVHLIAKHMNGGTVEEAVMAACREA
RGAYSLLILANDKMIAVRDPHGFRPLLLGKVGDAHVFASESCAFDLLEAELIRSVKPGEMIVIDKGKVHSYDMDMGKKRH
HCIFELIYFARPDSMVFGENVYFCRKAMGKQLATEAPVDADFVMPFPDSGVYCAVGYAQQSGLPYEHAMIRNHYVGRTFI
QPSQDMRDYSVRVKINPVKDMIKDKRIIIVDDSIVRGTTIRTRVKKLRELGAREVHFRVSCPPIKFPCFYGIDFASKGEL
IAANHSVREIERFIGLDSLHYISIEGLLKSVSTPDDYCLACFTGSYPLQCEGNLKSCLECGC

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=41.5354330708661, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=38.7580299785867, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1790167, Length=321, Percent_Identity=27.4143302180685, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=476, Percent_Identity=40.546218487395, Blast_Score=337, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=480, Percent_Identity=37.2916666666667, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=41.8219461697723, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=42.1166306695464, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI28573187, Length=486, Percent_Identity=39.7119341563786, Blast_Score=342, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51789; Mature: 51789

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREYCGVFGIYNHAEAARMTYFGLYALQHRGQESAGIVTWDGSALREERGMGLVPEVFN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCHHHHH
ERHLSKELKGDIAIGHTRYSTTGASLLRNAQPFRVRYKNMDIAIAHNGNLVNTVELRSRL
HHHHHHHHCCCEEEECEEECCHHHHHHHCCCCEEEEEECEEEEEEECCCEEEHHHHHHHH
EESGSIFQTTNDSEVIVHLIAKHMNGGTVEEAVMAACREARGAYSLLILANDKMIAVRDP
HHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEECC
HGFRPLLLGKVGDAHVFASESCAFDLLEAELIRSVKPGEMIVIDKGKVHSYDMDMGKKRH
CCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCHH
HCIFELIYFARPDSMVFGENVYFCRKAMGKQLATEAPVDADFVMPFPDSGVYCAVGYAQQ
HHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEHHH
SGLPYEHAMIRNHYVGRTFIQPSQDMRDYSVRVKINPVKDMIKDKRIIIVDDSIVRGTTI
CCCCHHHHHHHHCCCCCEECCCCCCCCCEEEEEEEEHHHHHHCCCEEEEECCCEECCCHH
RTRVKKLRELGAREVHFRVSCPPIKFPCFYGIDFASKGELIAANHSVREIERFIGLDSLH
HHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHCCCCEE
YISIEGLLKSVSTPDDYCLACFTGSYPLQCEGNLKSCLECGC
EEEHHHHHHHCCCCCCEEEEEECCCCCEEECCCHHHHHHCCC
>Mature Secondary Structure
MKREYCGVFGIYNHAEAARMTYFGLYALQHRGQESAGIVTWDGSALREERGMGLVPEVFN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCHHHHH
ERHLSKELKGDIAIGHTRYSTTGASLLRNAQPFRVRYKNMDIAIAHNGNLVNTVELRSRL
HHHHHHHHCCCEEEECEEECCHHHHHHHCCCCEEEEEECEEEEEEECCCEEEHHHHHHHH
EESGSIFQTTNDSEVIVHLIAKHMNGGTVEEAVMAACREARGAYSLLILANDKMIAVRDP
HHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEEECC
HGFRPLLLGKVGDAHVFASESCAFDLLEAELIRSVKPGEMIVIDKGKVHSYDMDMGKKRH
CCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCHH
HCIFELIYFARPDSMVFGENVYFCRKAMGKQLATEAPVDADFVMPFPDSGVYCAVGYAQQ
HHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEHHH
SGLPYEHAMIRNHYVGRTFIQPSQDMRDYSVRVKINPVKDMIKDKRIIIVDDSIVRGTTI
CCCCHHHHHHHHCCCCCEECCCCCCCCCEEEEEEEEHHHHHHCCCEEEEECCCEECCCHH
RTRVKKLRELGAREVHFRVSCPPIKFPCFYGIDFASKGELIAANHSVREIERFIGLDSLH
HHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHCCCCEE
YISIEGLLKSVSTPDDYCLACFTGSYPLQCEGNLKSCLECGC
EEEHHHHHHHCCCCCCEEEEEECCCCCEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]