Click here to switch to the map view.

The map label for this gene is kinE [H]

Identifier: 78355367

GI number: 78355367

Start: 309353

End: 311083

Strand: Direct

Name: kinE [H]

Synonym: Dde_0320

Alternate gene names: 78355367

Gene position: 309353-311083 (Clockwise)

Preceding gene: 78355366

Following gene: 78355368

Centisome position: 8.29

GC content: 55.63

Gene sequence:

>1731_bases
ATGGAGCAGCAACACTGCAGCAGACAGGGCTGGCTCGTCCGGCTGTTTGACCCGCTGGGCGGCACACAGTCGTCGCAGAG
GTATTATTATCTGCGGCGCAATATTATCATTCTGATTGTGTCCGTCTCGGTGGTGCCGCTGGCGCTGATGGCTGCCATAA
ACTTTTTCATGTATCAGGATTCGCTTAAAAATCAGGCGGAAAGCCAGGTGCGGACCCTGATAAACAAGACACGCCATTCT
TTTGAGCTTTTTCTGCATGAACGGCTGGCTTCCGTGGCTTTTATAGCCGGAGCCTATACCTACGATCAGCTTAAAGATGA
AAAAGCCCTGAACCGTGTGTACCACGCCGCCAAAAAAGAACTGGCCGGCTTTGTGGACTTCGGGCTTATAAACGCCAACG
GCACGCAGGTAAGCTATGTCGGTCCGTATGATCTTAAAAACAAAGATTATTCCGGTCAGGACTGGTTTCAGCAGGTCCGT
GTGCGCGGCACATACATCAGTGATGTGTTCATGGGATACCGCGGTATCCCGCATGTGGCCATAGCAGTGGAACATCTGGA
CGAAGACGGTGCGCGCTGGGTGGTGCGGGCCACCATCAACACCGACCGGTTTGAAGAACTCATAGCCGCCATGGGGCTGG
GCTCCCAGTCCGACGCTTTTCTTATCAACAGGGCCGGTGTGATGCAGACCGACTCGCGGCATTTCGGCAAGGTGCTGGAA
AAGGCTGCCATAACGGTGGGGCCCGGCACGCATCAGCCCGAAGTGGTGGAAAGCACCGGACTGAACGGGCAGAAGATTTT
TGTGGGGCATGTGAATTTCGGCAATCCGGAGCTGACGCTTGTGCTGGTGCAGCCCATAAGTGTGGCATACAAGGCGTGGA
ACAAGCTGCGCATGGAAATGCTCACGTTATTTCTGGGCAGCACGGTGCTGATTGTTTTTGTGGTCTGGGCTCTGACGGGC
GTGCTGGTGCGCCGCATACGCGAAGCGGACGACAAGCGTGCGGCGGCGTTCCGCGAGGTGGAACATGCGCACAAGCTGTC
TTCCATCGGACGGCTGGCTGCCGGAGTGGCACATGAAATAAACAACCCGCTTGCCATCATCAACGAGAAAGCAGGGCTGA
TGAATGACCTGATGGAGCACGGAAACGATGAGATCATCAGGGCGAAATTTCCCGAGCTTGTGGCCTCTGTGCTGCAGTCC
GTTTCGCGTTGCCGCACCATTACGCACCGGCTGCTGGGCTTTGCCCGCCGCATGGAAGTGCAGGTCGAACCGCTGGACGT
CAACGAGATTGTGCGCGAGGTGCTCGGTTTTCTGGAAAAAGAGAGCCTGTACCGCAATATCAGTCTTGAACTGAATCTGG
ATGACGATCTGCCGCGTATTTCATCCGACCGCGGACAGCTGCAGCAGGTTTTTCTGAATATCATCAATAACGCCATAGCG
GCAGTTGATAACGGCGGCAGGGTGCAGATAACCACCCGCTCCGTCGATCTGGATACCGTTGCCGTGCTGATAACGGACAA
CGGCGTGGGAATGTCTGATGAAGTGCTGAGTCATATATTCGAACCCTTCTTTACCACCAAGGGTGCGGAAGGCACCGGTC
TTGGACTGGCGATCACCTATGGTATCGTGCAGAAGCTGGGCGGGCGCATTGCGGTTGCCAGCGAGGAAGGCAAGGGATCG
ACGTTCACCATTGAACTGCCCAAGCGTGCAAAGCCGCAGGAGGAGTCTTAG

Upstream 100 bases:

>100_bases
TCGGCAGGCGCAGAGATGTCATAAACGCCTATCTGGCCAGAACGCTCCGTCCGGGGGCGGTTCTCGTTCCGCGTGACGAA
TAAGGAATAAAGGGGTTGCC

Downstream 100 bases:

>100_bases
GCGATGTCTGAGCTGAAGGTGCTGCTGGTTGACGATGAAATTGAATTTGCCGGAACGCTGGCTGAGCGGTTGCGGCTGCG
CGGATTGCAGGCCGATGTGA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MEQQHCSRQGWLVRLFDPLGGTQSSQRYYYLRRNIIILIVSVSVVPLALMAAINFFMYQDSLKNQAESQVRTLINKTRHS
FELFLHERLASVAFIAGAYTYDQLKDEKALNRVYHAAKKELAGFVDFGLINANGTQVSYVGPYDLKNKDYSGQDWFQQVR
VRGTYISDVFMGYRGIPHVAIAVEHLDEDGARWVVRATINTDRFEELIAAMGLGSQSDAFLINRAGVMQTDSRHFGKVLE
KAAITVGPGTHQPEVVESTGLNGQKIFVGHVNFGNPELTLVLVQPISVAYKAWNKLRMEMLTLFLGSTVLIVFVVWALTG
VLVRRIREADDKRAAAFREVEHAHKLSSIGRLAAGVAHEINNPLAIINEKAGLMNDLMEHGNDEIIRAKFPELVASVLQS
VSRCRTITHRLLGFARRMEVQVEPLDVNEIVREVLGFLEKESLYRNISLELNLDDDLPRISSDRGQLQQVFLNIINNAIA
AVDNGGRVQITTRSVDLDTVAVLITDNGVGMSDEVLSHIFEPFFTTKGAEGTGLGLAITYGIVQKLGGRIAVASEEGKGS
TFTIELPKRAKPQEES

Sequences:

>Translated_576_residues
MEQQHCSRQGWLVRLFDPLGGTQSSQRYYYLRRNIIILIVSVSVVPLALMAAINFFMYQDSLKNQAESQVRTLINKTRHS
FELFLHERLASVAFIAGAYTYDQLKDEKALNRVYHAAKKELAGFVDFGLINANGTQVSYVGPYDLKNKDYSGQDWFQQVR
VRGTYISDVFMGYRGIPHVAIAVEHLDEDGARWVVRATINTDRFEELIAAMGLGSQSDAFLINRAGVMQTDSRHFGKVLE
KAAITVGPGTHQPEVVESTGLNGQKIFVGHVNFGNPELTLVLVQPISVAYKAWNKLRMEMLTLFLGSTVLIVFVVWALTG
VLVRRIREADDKRAAAFREVEHAHKLSSIGRLAAGVAHEINNPLAIINEKAGLMNDLMEHGNDEIIRAKFPELVASVLQS
VSRCRTITHRLLGFARRMEVQVEPLDVNEIVREVLGFLEKESLYRNISLELNLDDDLPRISSDRGQLQQVFLNIINNAIA
AVDNGGRVQITTRSVDLDTVAVLITDNGVGMSDEVLSHIFEPFFTTKGAEGTGLGLAITYGIVQKLGGRIAVASEEGKGS
TFTIELPKRAKPQEES
>Mature_576_residues
MEQQHCSRQGWLVRLFDPLGGTQSSQRYYYLRRNIIILIVSVSVVPLALMAAINFFMYQDSLKNQAESQVRTLINKTRHS
FELFLHERLASVAFIAGAYTYDQLKDEKALNRVYHAAKKELAGFVDFGLINANGTQVSYVGPYDLKNKDYSGQDWFQQVR
VRGTYISDVFMGYRGIPHVAIAVEHLDEDGARWVVRATINTDRFEELIAAMGLGSQSDAFLINRAGVMQTDSRHFGKVLE
KAAITVGPGTHQPEVVESTGLNGQKIFVGHVNFGNPELTLVLVQPISVAYKAWNKLRMEMLTLFLGSTVLIVFVVWALTG
VLVRRIREADDKRAAAFREVEHAHKLSSIGRLAAGVAHEINNPLAIINEKAGLMNDLMEHGNDEIIRAKFPELVASVLQS
VSRCRTITHRLLGFARRMEVQVEPLDVNEIVREVLGFLEKESLYRNISLELNLDDDLPRISSDRGQLQQVFLNIINNAIA
AVDNGGRVQITTRSVDLDTVAVLITDNGVGMSDEVLSHIFEPFFTTKGAEGTGLGLAITYGIVQKLGGRIAVASEEGKGS
TFTIELPKRAKPQEES

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=280, Percent_Identity=35.7142857142857, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=36.1702127659575, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=29.8701298701299, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790300, Length=273, Percent_Identity=26.007326007326, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=29.0836653386454, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI48994928, Length=158, Percent_Identity=29.1139240506329, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=241, Percent_Identity=24.0663900414938, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790861, Length=223, Percent_Identity=25.5605381165919, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1786783, Length=227, Percent_Identity=25.1101321585903, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64069; Mature: 64069

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQHCSRQGWLVRLFDPLGGTQSSQRYYYLRRNIIILIVSVSVVPLALMAAINFFMYQD
CCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHH
SLKNQAESQVRTLINKTRHSFELFLHERLASVAFIAGAYTYDQLKDEKALNRVYHAAKKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGFVDFGLINANGTQVSYVGPYDLKNKDYSGQDWFQQVRVRGTYISDVFMGYRGIPHVA
HHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHE
IAVEHLDEDGARWVVRATINTDRFEELIAAMGLGSQSDAFLINRAGVMQTDSRHFGKVLE
EEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHH
KAAITVGPGTHQPEVVESTGLNGQKIFVGHVNFGNPELTLVLVQPISVAYKAWNKLRMEM
HHEEEECCCCCCCCHHHHCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHH
LTLFLGSTVLIVFVVWALTGVLVRRIREADDKRAAAFREVEHAHKLSSIGRLAAGVAHEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNPLAIINEKAGLMNDLMEHGNDEIIRAKFPELVASVLQSVSRCRTITHRLLGFARRMEV
CCCEEEEECCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
QVEPLDVNEIVREVLGFLEKESLYRNISLELNLDDDLPRISSDRGQLQQVFLNIINNAIA
EECCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
AVDNGGRVQITTRSVDLDTVAVLITDNGVGMSDEVLSHIFEPFFTTKGAEGTGLGLAITY
EECCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GIVQKLGGRIAVASEEGKGSTFTIELPKRAKPQEES
HHHHHHCCEEEEEECCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MEQQHCSRQGWLVRLFDPLGGTQSSQRYYYLRRNIIILIVSVSVVPLALMAAINFFMYQD
CCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHH
SLKNQAESQVRTLINKTRHSFELFLHERLASVAFIAGAYTYDQLKDEKALNRVYHAAKKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGFVDFGLINANGTQVSYVGPYDLKNKDYSGQDWFQQVRVRGTYISDVFMGYRGIPHVA
HHHHHHEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHE
IAVEHLDEDGARWVVRATINTDRFEELIAAMGLGSQSDAFLINRAGVMQTDSRHFGKVLE
EEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHH
KAAITVGPGTHQPEVVESTGLNGQKIFVGHVNFGNPELTLVLVQPISVAYKAWNKLRMEM
HHEEEECCCCCCCCHHHHCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHH
LTLFLGSTVLIVFVVWALTGVLVRRIREADDKRAAAFREVEHAHKLSSIGRLAAGVAHEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNPLAIINEKAGLMNDLMEHGNDEIIRAKFPELVASVLQSVSRCRTITHRLLGFARRMEV
CCCEEEEECCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
QVEPLDVNEIVREVLGFLEKESLYRNISLELNLDDDLPRISSDRGQLQQVFLNIINNAIA
EECCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
AVDNGGRVQITTRSVDLDTVAVLITDNGVGMSDEVLSHIFEPFFTTKGAEGTGLGLAITY
EECCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GIVQKLGGRIAVASEEGKGSTFTIELPKRAKPQEES
HHHHHHCCEEEEEECCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]