Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 78355307

GI number: 78355307

Start: 244822

End: 245790

Strand: Direct

Name: braE [H]

Synonym: Dde_0260

Alternate gene names: 78355307

Gene position: 244822-245790 (Clockwise)

Preceding gene: 78355306

Following gene: 78355308

Centisome position: 6.56

GC content: 57.89

Gene sequence:

>969_bases
ATGAACAGACGCTACGCGATGTACATGGTCGCTGCGGCGGCGTTTGCCACCATGCCGGCCTTTATCAACCCCTACTGGAC
CGATGTGCTCAACACCGTGGGACTGTATGCGGTTCTGGCTCTGTCGCTGAATATCATTCTGGGCCATGCGGGCATGTTTC
ACATGGGGCATGCCGCCTTTTACGCCGTGGGGGCCTACACCACGGCCGTTCTCAACGTGACATTTCATGTTCCCGTGCTG
TGGGCCATGCCTGCGGCCGGTGTGCTTGCGGCATTGTTTGCGCTGGTGGTGGCACGTCCCATCATTCATCTGCGGGGCGA
CTACCTGCTTATCGTGACCATAGGCATAGTGGAAATTGTGCGTATAGCGCTGGTGAACAACATTTTTGACATGACCGGCG
GCGCCAACGGCATTTTCGGCATAGCCCGGCCGGAACTGTTCGGTTTCAAGATCCGCAAGCCCGTGCATTTTTTCTATCTT
ATCTGGGGCTTCTGCGCTGTGACCATCCTGCTGTTCCACTGGCTCGAAAATTCGCGTTTCGGCAGAGCGCTCAACTACAT
ACGTGAAGACGAAGTGGCCGCGGAAGGCAGCGGAGTGAACACCGCAGCCTACAAGCTGGGTGCTTTTGTGCTGGGTGCTT
TCTGGGCGGGTATGGCCGGAACTCTCTTTGCCGCTAAAATGACCATCATCTCACCGGAATCATTTTCTTTCTGGGAATCT
GTGGTCATGTTCACCATTGTGATTCTGGGCGGCATGGGTTCCATCCGCGGGGTGTTGCTGGGGGCCTTTCTCATCGTCGG
CCTGCCCGAAGTTTTCCGCGAGCTGGCAAGCGCCCGTATGCTGGTGTTCGGTGCGGCCATGGTGGCCATGATGATTTTCC
GTACGCAGGGCCTGCTGCCTCCGGCACCGCGCCATTATGACCTGCGGGGAATCATGTCCGGCACGCAGACGCGGGAGGAG
CGCGCATGA

Upstream 100 bases:

>100_bases
TTTCCATCGCCTGGAAAGATGCCATCGCCTTCTTCGTGCTCATCATCATTCTGATAATCAGGCCGACAGGCCTGCTGGGC
GAAAGGGTGGCTGACAAAAT

Downstream 100 bases:

>100_bases
GCCTGCTCAGACTGACCAACCTTACCAAGACCTTCGGCGGGCTTATTGCCGTCAATGATGTTTCCATGACTGTGGAGCAG
GGCAGCATTGTGGGGTTGAT

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNRRYAMYMVAAAAFATMPAFINPYWTDVLNTVGLYAVLALSLNIILGHAGMFHMGHAAFYAVGAYTTAVLNVTFHVPVL
WAMPAAGVLAALFALVVARPIIHLRGDYLLIVTIGIVEIVRIALVNNIFDMTGGANGIFGIARPELFGFKIRKPVHFFYL
IWGFCAVTILLFHWLENSRFGRALNYIREDEVAAEGSGVNTAAYKLGAFVLGAFWAGMAGTLFAAKMTIISPESFSFWES
VVMFTIVILGGMGSIRGVLLGAFLIVGLPEVFRELASARMLVFGAAMVAMMIFRTQGLLPPAPRHYDLRGIMSGTQTREE
RA

Sequences:

>Translated_322_residues
MNRRYAMYMVAAAAFATMPAFINPYWTDVLNTVGLYAVLALSLNIILGHAGMFHMGHAAFYAVGAYTTAVLNVTFHVPVL
WAMPAAGVLAALFALVVARPIIHLRGDYLLIVTIGIVEIVRIALVNNIFDMTGGANGIFGIARPELFGFKIRKPVHFFYL
IWGFCAVTILLFHWLENSRFGRALNYIREDEVAAEGSGVNTAAYKLGAFVLGAFWAGMAGTLFAAKMTIISPESFSFWES
VVMFTIVILGGMGSIRGVLLGAFLIVGLPEVFRELASARMLVFGAAMVAMMIFRTQGLLPPAPRHYDLRGIMSGTQTREE
RA
>Mature_322_residues
MNRRYAMYMVAAAAFATMPAFINPYWTDVLNTVGLYAVLALSLNIILGHAGMFHMGHAAFYAVGAYTTAVLNVTFHVPVL
WAMPAAGVLAALFALVVARPIIHLRGDYLLIVTIGIVEIVRIALVNNIFDMTGGANGIFGIARPELFGFKIRKPVHFFYL
IWGFCAVTILLFHWLENSRFGRALNYIREDEVAAEGSGVNTAAYKLGAFVLGAFWAGMAGTLFAAKMTIISPESFSFWES
VVMFTIVILGGMGSIRGVLLGAFLIVGLPEVFRELASARMLVFGAAMVAMMIFRTQGLLPPAPRHYDLRGIMSGTQTREE
RA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=313, Percent_Identity=39.6166134185303, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35173; Mature: 35173

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRYAMYMVAAAAFATMPAFINPYWTDVLNTVGLYAVLALSLNIILGHAGMFHMGHAAF
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAVGAYTTAVLNVTFHVPVLWAMPAAGVLAALFALVVARPIIHLRGDYLLIVTIGIVEIV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
RIALVNNIFDMTGGANGIFGIARPELFGFKIRKPVHFFYLIWGFCAVTILLFHWLENSRF
HHHHHHHHHHHCCCCCCCEECCCCHHHCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
GRALNYIREDEVAAEGSGVNTAAYKLGAFVLGAFWAGMAGTLFAAKMTIISPESFSFWES
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
VVMFTIVILGGMGSIRGVLLGAFLIVGLPEVFRELASARMLVFGAAMVAMMIFRTQGLLP
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAPRHYDLRGIMSGTQTREERA
CCCCCCCHHHHHCCCCHHHCCC
>Mature Secondary Structure
MNRRYAMYMVAAAAFATMPAFINPYWTDVLNTVGLYAVLALSLNIILGHAGMFHMGHAAF
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAVGAYTTAVLNVTFHVPVLWAMPAAGVLAALFALVVARPIIHLRGDYLLIVTIGIVEIV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHH
RIALVNNIFDMTGGANGIFGIARPELFGFKIRKPVHFFYLIWGFCAVTILLFHWLENSRF
HHHHHHHHHHHCCCCCCCEECCCCHHHCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
GRALNYIREDEVAAEGSGVNTAAYKLGAFVLGAFWAGMAGTLFAAKMTIISPESFSFWES
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHH
VVMFTIVILGGMGSIRGVLLGAFLIVGLPEVFRELASARMLVFGAAMVAMMIFRTQGLLP
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PAPRHYDLRGIMSGTQTREERA
CCCCCCCHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]