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The map label for this gene is pit [H]

Identifier: 78224323

GI number: 78224323

Start: 3527965

End: 3528966

Strand: Reverse

Name: pit [H]

Synonym: Gmet_3131

Alternate gene names: 78224323

Gene position: 3528966-3527965 (Counterclockwise)

Preceding gene: 78224324

Following gene: 78224321

Centisome position: 88.28

GC content: 60.98

Gene sequence:

>1002_bases
ATGCTTGATGCCACGTTGATCATGCTCGTCCTGGTCATCGGAGCGGCGCTGGCGTTCGACTACATCAACGGCTTCCACGA
TACGGCAAATGCCATCGCCACCTGCGTCTCGACCCGGGCACTCTCCGTCAGGGCCGCCATTATCATGGCGGCGGTGCTGA
ACTTCGCCGGCGCCATGATCTCCACCAAGGTGGCCGCCACCATCGGCAAGGGGATCGTCGATGCGCCCAACGTTACCCAG
ATGGTGGTCCTGGCGGGGATCCTCGGCGCCATTGTCTGGGATTTGATCACCTGGTACTACGGACTCCCCGCCTCCTCCTC
CCACGCCATCATCGGTGGACTCATGGGTGCGGTGATCGCCCATGCCGGCACGACGGCCCTCCACTGGAAAGGCCTCGAAA
AGATCGTCCTCTCCCTGGTCCTTTCCCCCATCGTGGGAACCTTCCTCGGTTTCGCCGTCATGGTCATCATGCTCTGGTGT
TTTAAAAGCACATCACCCTATTCCATTAATAAACACTTCCGGCGACTTCAGGTTCTCTCGGCGGCCTTCATGGCCTTCTC
CCACGGCACCGCCGACGCCCAGAAGTCCATGGGGGTCATCACCATGGCGCTGGTGAGCTACGGAGCCCTCTCCTCCTTCG
TTGTTCCCACCTGGGTGAAGATTGCCTGCGCCGTGGCCATGGGGCTCGGCACCGCCGCCGGCGGGTGGCGGATCATCAAG
ACCGTCGGCAAAGACTTCGTGAAGCTTCAGCCCGTTCACGGCTTCTGTGTCGAAACCGCCTCGGCCGGGGTCATTCTGGG
GGCGTCATCCATCGGGATGCCGGTCAGTACTACTCACGTCATCACGTCCGCAATTCTCGGCGTTGGTCTCTCAAAGCGCC
TCACAGCGGTCAACTGGAACGTGGCCGGGAGGATAGTCGTCGCCTGGGTCCTGACCATCCCGGCATCGGCGATCATGGCC
TATGTCTGCTACCAGGTTCTCAATCCTATTCTTGGGAAGTGA

Upstream 100 bases:

>100_bases
ATCCAGCTCATCAAGTGGAAAGAGATCTACGAGACCCTGGAGCGGGCAACCGACAAGTGCGAGGACGCGGCCAACATTCT
GGAAAGCGTGGTGGTGAAAA

Downstream 100 bases:

>100_bases
GCGGAGTTGCCGCTGAACAGTGGCGGGTGACAAATCGACAGGAAACAGAAAAAGCGGGGCATCGTCCCCGCTTTTTCTAT
TCCAGCTCTTCGCCGACCTC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLDATLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRAAIIMAAVLNFAGAMISTKVAATIGKGIVDAPNVTQ
MVVLAGILGAIVWDLITWYYGLPASSSHAIIGGLMGAVIAHAGTTALHWKGLEKIVLSLVLSPIVGTFLGFAVMVIMLWC
FKSTSPYSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGALSSFVVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSAILGVGLSKRLTAVNWNVAGRIVVAWVLTIPASAIMA
YVCYQVLNPILGK

Sequences:

>Translated_333_residues
MLDATLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRAAIIMAAVLNFAGAMISTKVAATIGKGIVDAPNVTQ
MVVLAGILGAIVWDLITWYYGLPASSSHAIIGGLMGAVIAHAGTTALHWKGLEKIVLSLVLSPIVGTFLGFAVMVIMLWC
FKSTSPYSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGALSSFVVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSAILGVGLSKRLTAVNWNVAGRIVVAWVLTIPASAIMA
YVCYQVLNPILGK
>Mature_333_residues
MLDATLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRAAIIMAAVLNFAGAMISTKVAATIGKGIVDAPNVTQ
MVVLAGILGAIVWDLITWYYGLPASSSHAIIGGLMGAVIAHAGTTALHWKGLEKIVLSLVLSPIVGTFLGFAVMVIMLWC
FKSTSPYSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGALSSFVVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSAILGVGLSKRLTAVNWNVAGRIVVAWVLTIPASAIMA
YVCYQVLNPILGK

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=36.5853658536585, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI5803173, Length=163, Percent_Identity=35.5828220858896, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789907, Length=227, Percent_Identity=34.3612334801762, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789360, Length=227, Percent_Identity=34.3612334801762, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71981576, Length=156, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=29.6969696969697, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25146401, Length=156, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=167, Percent_Identity=28.7425149700599, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32566716, Length=153, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=151, Percent_Identity=34.4370860927152, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356511, Length=176, Percent_Identity=26.7045454545455, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34708; Mature: 34708

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]