Click here to switch to the map view.

The map label for this gene is fliF [H]

Identifier: 78224304

GI number: 78224304

Start: 3510531

End: 3512114

Strand: Reverse

Name: fliF [H]

Synonym: Gmet_3112

Alternate gene names: 78224304

Gene position: 3512114-3510531 (Counterclockwise)

Preceding gene: 78224305

Following gene: 78224303

Centisome position: 87.86

GC content: 64.02

Gene sequence:

>1584_bases
ATGCCTGAAGGTCTGAAAAAATTGTTGGAGCCGTTCATGGCCCTTCCCCCCGCGAAGCGGTGGATCGTGGGCGGGGTCGT
GGGGGTGTCGATCATCGCCTTCGCCCTCCTGATAATGGTCGCCAACCGGACCGACTACCGGCCCCTCTTCACCAACCTCA
CCACCGAGGATGCCGGCGAGATCGTCACCAAGCTCAAGGAGCAGAAGGTTCCCTACCGGATCGCCGCCGACGGCAAGGCA
ATCCTGGTCCCCTCCGACAAGGTCTACGATCTGCGCCTGACCATGGCCTCCGCAGGGCTTCCCCAGGGGGGCGGGGTCGG
CTTCGAGATCTTTGACCGAAAAAACTTTGGCATGACCGAGTTCGTCCAGAAGCTCAACTACCAGCGGGCCCTCCAGGGGG
AACTCTCCCGGACCATCGGCCAGATCGCCGGGGTGGAGCAGGCTCGGGTCCACCTGGCAATTCCTGAAAAGGCCCTCTTC
AAGGAGGACGAGAAGCCCGCCACCGCATCGGTGGTGCTCAAGGTGAAGGGGAATCGCCAACTCAATGACAGTGATGTTCA
GGGGGTCGTCCATCTCGTGGCTTCCTCCATCGAGGGGATGAACCCGGAGCACGTGACCGTCCTCGACCAGAAGGGGAAGC
TCCTTTCCAAGAATGGACCCACTGACGCCGCCGGCAAGATGACCGCCTCCATGCAGGAGGTGCAGCGGACCTACGAGCGG
GGCACCGAGGAGCGGCTCCAGTCGCTCCTGGACAAGGCGGTCGGTTCTGGCAAGTCGGTGGCCCGCGTGTCGGCGGTCTT
CGATTTCCGGCAGGTGGAGCGCTACGAGGAGAAGTACGACCCCGAAACCGTGGTCCGCAGCGAGCAGCGCAGCGAGGAGA
GCATGGGAGGCGCCAACGCCACGGCGGGAGTCCCGGGGGCACAGACGAACCTGGGCCGCACCCCGGCCGGCGCCGGCGGC
GCCACCGGCCCTAACGGAAGCAAGAACGATGAAACCCTCAACTACGAGGTGAGCCGCTCCACGGCCCGGACCATCGAGCC
GGTGGGAGCCCTTTCCAAGGTGTCGGTGGCGATCCTCGTGGACGGCAAGTACGACGCCCCGGCCGTCGGCAAGGACGGCA
AGAACGCCAAGCCCAAGTACACCCCCCGCTCCCCCGCCGAACTCCAACAGATTGAGGCTCTGGTCAAAGGCGCCGTCGGC
TTCAACGCCGTCAGGGGTGATCAGGTGACGGTGGCGAACATCCCGTTCCAGGATACCGCCGAGGCGGGCACCGACGAGGG
GAGCCAGTGGTGGAACGCGCCGATCTTCCTCTCGCTCCTCAAGAACGGCCTCATCGGCTTCGGCTTCCTGGCACTCCTCC
TCTTTGTGGTACGCCCCCTCATGAAGATGCTGAAGCCCGAGAAGAAGACCTCCTTCGAGCCGATTTCCACTGCCGAGGAA
ACCATCAACCAGATCGCCGAGGTCCACCGCGCCCAGATCCAGAACCAGACCATCCGGCAGGTGGAACTCATGAACAAAGT
GAAGGAAGAGCCCTACCAGGCCGCCCAGATCCTCCAGAACTGGCTCCGCGCCAAGGAACAGTGA

Upstream 100 bases:

>100_bases
TGCTACGCTTCACTCCTCGGGTTCGGGCGCCTAGCATCTGGGCATTTTTGAGCGCTCACGGTAAGAGCGGAATTCTATAA
GGTTTAACCCGGAGTCATAA

Downstream 100 bases:

>100_bases
TCCGTTAGCATGAACCACCATCCCATCCCACGTTTTCCGAGGCTCTGACCATGGACGGTACCGACAAGGCTGCCATTCTG
CTCCTCTACCTGGGTCCCGA

Product: flagellar FliF M-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MPEGLKKLLEPFMALPPAKRWIVGGVVGVSIIAFALLIMVANRTDYRPLFTNLTTEDAGEIVTKLKEQKVPYRIAADGKA
ILVPSDKVYDLRLTMASAGLPQGGGVGFEIFDRKNFGMTEFVQKLNYQRALQGELSRTIGQIAGVEQARVHLAIPEKALF
KEDEKPATASVVLKVKGNRQLNDSDVQGVVHLVASSIEGMNPEHVTVLDQKGKLLSKNGPTDAAGKMTASMQEVQRTYER
GTEERLQSLLDKAVGSGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEESMGGANATAGVPGAQTNLGRTPAGAGG
ATGPNGSKNDETLNYEVSRSTARTIEPVGALSKVSVAILVDGKYDAPAVGKDGKNAKPKYTPRSPAELQQIEALVKGAVG
FNAVRGDQVTVANIPFQDTAEAGTDEGSQWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLMKMLKPEKKTSFEPISTAEE
TINQIAEVHRAQIQNQTIRQVELMNKVKEEPYQAAQILQNWLRAKEQ

Sequences:

>Translated_527_residues
MPEGLKKLLEPFMALPPAKRWIVGGVVGVSIIAFALLIMVANRTDYRPLFTNLTTEDAGEIVTKLKEQKVPYRIAADGKA
ILVPSDKVYDLRLTMASAGLPQGGGVGFEIFDRKNFGMTEFVQKLNYQRALQGELSRTIGQIAGVEQARVHLAIPEKALF
KEDEKPATASVVLKVKGNRQLNDSDVQGVVHLVASSIEGMNPEHVTVLDQKGKLLSKNGPTDAAGKMTASMQEVQRTYER
GTEERLQSLLDKAVGSGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEESMGGANATAGVPGAQTNLGRTPAGAGG
ATGPNGSKNDETLNYEVSRSTARTIEPVGALSKVSVAILVDGKYDAPAVGKDGKNAKPKYTPRSPAELQQIEALVKGAVG
FNAVRGDQVTVANIPFQDTAEAGTDEGSQWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLMKMLKPEKKTSFEPISTAEE
TINQIAEVHRAQIQNQTIRQVELMNKVKEEPYQAAQILQNWLRAKEQ
>Mature_526_residues
PEGLKKLLEPFMALPPAKRWIVGGVVGVSIIAFALLIMVANRTDYRPLFTNLTTEDAGEIVTKLKEQKVPYRIAADGKAI
LVPSDKVYDLRLTMASAGLPQGGGVGFEIFDRKNFGMTEFVQKLNYQRALQGELSRTIGQIAGVEQARVHLAIPEKALFK
EDEKPATASVVLKVKGNRQLNDSDVQGVVHLVASSIEGMNPEHVTVLDQKGKLLSKNGPTDAAGKMTASMQEVQRTYERG
TEERLQSLLDKAVGSGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEESMGGANATAGVPGAQTNLGRTPAGAGGA
TGPNGSKNDETLNYEVSRSTARTIEPVGALSKVSVAILVDGKYDAPAVGKDGKNAKPKYTPRSPAELQQIEALVKGAVGF
NAVRGDQVTVANIPFQDTAEAGTDEGSQWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLMKMLKPEKKTSFEPISTAEET
INQIAEVHRAQIQNQTIRQVELMNKVKEEPYQAAQILQNWLRAKEQ

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=449, Percent_Identity=36.9710467706013, Blast_Score=272, Evalue=4e-74,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 57314; Mature: 57183

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGLKKLLEPFMALPPAKRWIVGGVVGVSIIAFALLIMVANRTDYRPLFTNLTTEDAGE
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHH
IVTKLKEQKVPYRIAADGKAILVPSDKVYDLRLTMASAGLPQGGGVGFEIFDRKNFGMTE
HHHHHHHHCCCEEEECCCCEEEECCCCEEEEEEEHHHCCCCCCCCCCEEEECCCCCCHHH
FVQKLNYQRALQGELSRTIGQIAGVEQARVHLAIPEKALFKEDEKPATASVVLKVKGNRQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCEEEEEEECCCCC
LNDSDVQGVVHLVASSIEGMNPEHVTVLDQKGKLLSKNGPTDAAGKMTASMQEVQRTYER
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHC
GTEERLQSLLDKAVGSGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEESMGGANA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
TAGVPGAQTNLGRTPAGAGGATGPNGSKNDETLNYEVSRSTARTIEPVGALSKVSVAILV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHEEEEEEE
DGKYDAPAVGKDGKNAKPKYTPRSPAELQQIEALVKGAVGFNAVRGDQVTVANIPFQDTA
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
EAGTDEGSQWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLMKMLKPEKKTSFEPISTAEE
CCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
TINQIAEVHRAQIQNQTIRQVELMNKVKEEPYQAAQILQNWLRAKEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEGLKKLLEPFMALPPAKRWIVGGVVGVSIIAFALLIMVANRTDYRPLFTNLTTEDAGE
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHH
IVTKLKEQKVPYRIAADGKAILVPSDKVYDLRLTMASAGLPQGGGVGFEIFDRKNFGMTE
HHHHHHHHCCCEEEECCCCEEEECCCCEEEEEEEHHHCCCCCCCCCCEEEECCCCCCHHH
FVQKLNYQRALQGELSRTIGQIAGVEQARVHLAIPEKALFKEDEKPATASVVLKVKGNRQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCEEEEEEECCCCC
LNDSDVQGVVHLVASSIEGMNPEHVTVLDQKGKLLSKNGPTDAAGKMTASMQEVQRTYER
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHC
GTEERLQSLLDKAVGSGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEESMGGANA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
TAGVPGAQTNLGRTPAGAGGATGPNGSKNDETLNYEVSRSTARTIEPVGALSKVSVAILV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCHHHEEEEEEE
DGKYDAPAVGKDGKNAKPKYTPRSPAELQQIEALVKGAVGFNAVRGDQVTVANIPFQDTA
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
EAGTDEGSQWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLMKMLKPEKKTSFEPISTAEE
CCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
TINQIAEVHRAQIQNQTIRQVELMNKVKEEPYQAAQILQNWLRAKEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]