Click here to switch to the map view.

The map label for this gene is fliM [H]

Identifier: 78224292

GI number: 78224292

Start: 3498729

End: 3499712

Strand: Reverse

Name: fliM [H]

Synonym: Gmet_3100

Alternate gene names: 78224292

Gene position: 3499712-3498729 (Counterclockwise)

Preceding gene: 78224293

Following gene: 78224291

Centisome position: 87.55

GC content: 58.74

Gene sequence:

>984_bases
ATGGAAAAGATCCTCACAAAACAAGAAATAGAGGCGCTGCTGGCCGCCGTCTTCGAGGGGAAGATCGAGCCCGACAAGGA
GCTGGCCAAGGCAGAGGCGACGGTTCAGTCCTACGACCTCTTCAGTAGTGAGTCGAAGGGGAACATTCCCAACCTCGACA
TCATTTACGACAGCTTCATCCGCTACCAGCGGGGCACCCTGTCGAACCGCCTGGGGCGCATCGTCGAGATCAAGAAGGTG
GGGGCAGCTTCCTTCAAGTTCGACGACTTCCTCCATACCATGCCGTCGCCGGTGGCCATGGCCGTCTACAAGGCCGATCC
CCTCAAGGGGGCGGCGCTGATCGCCTTTGACAGCACCCTGGTCTTCACCATCGTCGACTGCATTCTGGGGGGGACCGGTT
CCTCGGTCGTGCCGACCGTCGGCAACCGGATGTTCACCTCCATCGAGTTGCGGCTGGTGCAGAAGATCGTCATGGATGTG
CTTGCCGATCTCGAAAAGGCCTGGGCCCCCATTTACGCCACCAAAATGGGCTTTTTGCGGATGGAGATGAACCCCCGGCT
CGTCAATATCGTTCCCCCCGAGTATCAGGTGGTGACGATGGAGATGGAGATTCAGATCGAGGAGATCATCGGCAAGATGA
TCTTCGCCGTACCCCTCACGACCATCGATCCGGTCAGGGAAAAGCTCAAGAACGGCGCCCAGGTCGACATGATGGCCATC
GATCCCCAGTGGTCGTTCCGCCTGTCGAAGGAACTTCTGGAGGCCCCCCTCGACCTTTCCGTGGAGGTGGGAGGAGCCAT
CATCAGCATGGACGAGCTTCTGAATCTCACCCCTGGCGACACCATCATGCTTGACACACCCTGCAACAGCGACCTGGTGG
TGAAGGTTGGGGGGGTATCGAAATTCATGTCCGCACCGGGGCTGAGGCACGGCAACAAGGCGGCGCAGATAACCACCATT
ATCGGTAAAGGGAGAGAGCAGTGA

Upstream 100 bases:

>100_bases
CCTCCCTCCGGGCAAGGTGAGCAAGGTCTATTTCACCGACTTCGTGGTGCAGTAGGGGCGCCGCTTGCCGTGTGATTCGC
CGTAACACCAGGAACCGTCC

Downstream 100 bases:

>100_bases
GCGACCTCGACAATGAAAACCTGAAGGATGGCGAACTGGATACCAAGAACCTGAACTTCATCCTCGACATACCGCTGCAG
CTCACGGTGGAGCTGGGGCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MEKILTKQEIEALLAAVFEGKIEPDKELAKAEATVQSYDLFSSESKGNIPNLDIIYDSFIRYQRGTLSNRLGRIVEIKKV
GAASFKFDDFLHTMPSPVAMAVYKADPLKGAALIAFDSTLVFTIVDCILGGTGSSVVPTVGNRMFTSIELRLVQKIVMDV
LADLEKAWAPIYATKMGFLRMEMNPRLVNIVPPEYQVVTMEMEIQIEEIIGKMIFAVPLTTIDPVREKLKNGAQVDMMAI
DPQWSFRLSKELLEAPLDLSVEVGGAIISMDELLNLTPGDTIMLDTPCNSDLVVKVGGVSKFMSAPGLRHGNKAAQITTI
IGKGREQ

Sequences:

>Translated_327_residues
MEKILTKQEIEALLAAVFEGKIEPDKELAKAEATVQSYDLFSSESKGNIPNLDIIYDSFIRYQRGTLSNRLGRIVEIKKV
GAASFKFDDFLHTMPSPVAMAVYKADPLKGAALIAFDSTLVFTIVDCILGGTGSSVVPTVGNRMFTSIELRLVQKIVMDV
LADLEKAWAPIYATKMGFLRMEMNPRLVNIVPPEYQVVTMEMEIQIEEIIGKMIFAVPLTTIDPVREKLKNGAQVDMMAI
DPQWSFRLSKELLEAPLDLSVEVGGAIISMDELLNLTPGDTIMLDTPCNSDLVVKVGGVSKFMSAPGLRHGNKAAQITTI
IGKGREQ
>Mature_327_residues
MEKILTKQEIEALLAAVFEGKIEPDKELAKAEATVQSYDLFSSESKGNIPNLDIIYDSFIRYQRGTLSNRLGRIVEIKKV
GAASFKFDDFLHTMPSPVAMAVYKADPLKGAALIAFDSTLVFTIVDCILGGTGSSVVPTVGNRMFTSIELRLVQKIVMDV
LADLEKAWAPIYATKMGFLRMEMNPRLVNIVPPEYQVVTMEMEIQIEEIIGKMIFAVPLTTIDPVREKLKNGAQVDMMAI
DPQWSFRLSKELLEAPLDLSVEVGGAIISMDELLNLTPGDTIMLDTPCNSDLVVKVGGVSKFMSAPGLRHGNKAAQITTI
IGKGREQ

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=28.5276073619632, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 35944; Mature: 35944

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKILTKQEIEALLAAVFEGKIEPDKELAKAEATVQSYDLFSSESKGNIPNLDIIYDSFI
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RYQRGTLSNRLGRIVEIKKVGAASFKFDDFLHTMPSPVAMAVYKADPLKGAALIAFDSTL
HHHCCCHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCHH
VFTIVDCILGGTGSSVVPTVGNRMFTSIELRLVQKIVMDVLADLEKAWAPIYATKMGFLR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCEEE
MEMNPRLVNIVPPEYQVVTMEMEIQIEEIIGKMIFAVPLTTIDPVREKLKNGAQVDMMAI
EECCCEEEEECCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
DPQWSFRLSKELLEAPLDLSVEVGGAIISMDELLNLTPGDTIMLDTPCNSDLVVKVGGVS
CCCCCHHHHHHHHCCCCCCEEECCCEEEEHHHHHCCCCCCEEEEECCCCCCEEEEECCHH
KFMSAPGLRHGNKAAQITTIIGKGREQ
HHHCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MEKILTKQEIEALLAAVFEGKIEPDKELAKAEATVQSYDLFSSESKGNIPNLDIIYDSFI
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RYQRGTLSNRLGRIVEIKKVGAASFKFDDFLHTMPSPVAMAVYKADPLKGAALIAFDSTL
HHHCCCHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCHH
VFTIVDCILGGTGSSVVPTVGNRMFTSIELRLVQKIVMDVLADLEKAWAPIYATKMGFLR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCEEE
MEMNPRLVNIVPPEYQVVTMEMEIQIEEIIGKMIFAVPLTTIDPVREKLKNGAQVDMMAI
EECCCEEEEECCCCEEEEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEE
DPQWSFRLSKELLEAPLDLSVEVGGAIISMDELLNLTPGDTIMLDTPCNSDLVVKVGGVS
CCCCCHHHHHHHHCCCCCCEEECCCEEEEHHHHHCCCCCCEEEEECCCCCCEEEEECCHH
KFMSAPGLRHGNKAAQITTIIGKGREQ
HHHCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]