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The map label for this gene is aspA

Identifier: 78224274

GI number: 78224274

Start: 3478370

End: 3479779

Strand: Reverse

Name: aspA

Synonym: Gmet_3082

Alternate gene names: 78224274

Gene position: 3479779-3478370 (Counterclockwise)

Preceding gene: 78224275

Following gene: 78224273

Centisome position: 87.05

GC content: 68.3

Gene sequence:

>1410_bases
GTGACGTTTCGGATTGAGAAGGATACCCTCGGCGACGTGCAGGTCCCGGCCGGCGCCTACTACGGTGCCCAGACGGCCAG
GGCCGTGGCCAACTTTCCCCTCTCGGGGCTCCGGCCCCATCCGGCCCTGGTTCGGGCCACGGTCGTCATAAAAAAGAGCG
CCGCCCGGGCCAATATGACCACGGGGAGGCTCGACCCCCGGCTCGGCGAGGCCATCGTCCGGGCCGCCGACGAGGCCCTC
GCCGGCGACTTCGATCCCCACTTCGTGGTGGACCCGTTCCAGGCCGGGGCCGGCACCTCCCACAATATGAACGTGAACGA
GATCCTCGCCAACCGGGCCAACGAACTCCTGGGGGGAGAGCGCGGCGCCTACGCGCCGGTCCACCCCAACGACCACGTGA
ACATGGCCCAGTCCACCAACGACGTCTTTCCCACGGCCATGCGGCTGGCGTCTCTTTCACTCGTGGAGAAGCTGCGTCCG
GGACTGGAGGGGCTCGTGGCGGCCCTGCGGGGGAAGGGGCGGGAGTTCGACGCCGTCCTCAAGAGCGGCCGGACCCACCT
CCAGGACGCGGTGCCGATTCGCCTCGGCCAGGAGTTCGAGGCGTGGGCCGTGGCAATGGAGAAAAACCTGGCGGGGATCG
ACGCCGCGCTGCCGGGGCTCCGGGAACTGGGGATCGGCGGCACCGCCGTCGGCACCGGCATGAACGCCGAGGCGGCCTAC
ATCGATCTGGTGGTGGCGGAACTGGCCCGGGAAACCGGGTTTCCTCTTCATCGCGGGACGAACCTGGTGGAGCGGATGCA
GAACATGGACCCCTTCGTGGCTCTTTCGTCGGCCCTCAAGGGGCTTGCCGCGAACCTCGTCCGCATCGCCAACGACCTGC
GGCTCCTCTCCTCCGGCCCCCGGACGGGGCTCGCCGAGATCGCGCTTCCGGCGATCCAGCCCGGCTCCTCCATCATGCCG
GGGAAGGTGAACCCGAGCATGGCCGAGGCAACCGACATGGTTGCCTTCCAGGTGATGGGGGCCGATGCGGTCATAACCCT
GGCAGCCCAGGCGGGACAGCTGGAGCTGAACGTGATGATGCCGGTCATCGCCTTCAATCTTCTCTTCAGCCTGGAGATCC
TGAAGAACACGGTGCCAAGGCTGGTCCAGGCGTGCATCGCCGGCATCACCGCCGACCGGGAGCGCTGCCGCCGGTACCTG
GACCAGTCGGTGGGGCTCGCCACGGTGCTGGCCCCCTACGTCGGCTACGCCGCCGCCGCCGAGGTGGCAAAGGAGTCGAC
GGCCACGGGACGGAGCATCCGGGAGATCGTGGAGGAGCGGCAGCTGCTGACGGCCGACAAGCTCGCGGAGATTCTCGACC
CCCTTCCCCTTACGAGCCCCGGGGTTCCGGGAGAGAAGCATAACGATTAA

Upstream 100 bases:

>100_bases
GGACGAGGAGACCGAGCACTTGCGGGTGATTACCGAGAAGTACAACGCCATCAAGGGGTAATCGGGGACCTGTTCCCGGG
GACCGGGAAGGGGCGAAACA

Downstream 100 bases:

>100_bases
GTGTTGAGTGCCACGACATCGTGGACAGAAAAATGTGCCAGCACATCGTAGACAAAGTTCTCTGACAATCGAATAGTCGC
CACGGCATTCTGTGAACACC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTFRIEKDTLGDVQVPAGAYYGAQTARAVANFPLSGLRPHPALVRATVVIKKSAARANMTTGRLDPRLGEAIVRAADEAL
AGDFDPHFVVDPFQAGAGTSHNMNVNEILANRANELLGGERGAYAPVHPNDHVNMAQSTNDVFPTAMRLASLSLVEKLRP
GLEGLVAALRGKGREFDAVLKSGRTHLQDAVPIRLGQEFEAWAVAMEKNLAGIDAALPGLRELGIGGTAVGTGMNAEAAY
IDLVVAELARETGFPLHRGTNLVERMQNMDPFVALSSALKGLAANLVRIANDLRLLSSGPRTGLAEIALPAIQPGSSIMP
GKVNPSMAEATDMVAFQVMGADAVITLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPRLVQACIAGITADRERCRRYL
DQSVGLATVLAPYVGYAAAAEVAKESTATGRSIREIVEERQLLTADKLAEILDPLPLTSPGVPGEKHND

Sequences:

>Translated_469_residues
MTFRIEKDTLGDVQVPAGAYYGAQTARAVANFPLSGLRPHPALVRATVVIKKSAARANMTTGRLDPRLGEAIVRAADEAL
AGDFDPHFVVDPFQAGAGTSHNMNVNEILANRANELLGGERGAYAPVHPNDHVNMAQSTNDVFPTAMRLASLSLVEKLRP
GLEGLVAALRGKGREFDAVLKSGRTHLQDAVPIRLGQEFEAWAVAMEKNLAGIDAALPGLRELGIGGTAVGTGMNAEAAY
IDLVVAELARETGFPLHRGTNLVERMQNMDPFVALSSALKGLAANLVRIANDLRLLSSGPRTGLAEIALPAIQPGSSIMP
GKVNPSMAEATDMVAFQVMGADAVITLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPRLVQACIAGITADRERCRRYL
DQSVGLATVLAPYVGYAAAAEVAKESTATGRSIREIVEERQLLTADKLAEILDPLPLTSPGVPGEKHND
>Mature_468_residues
TFRIEKDTLGDVQVPAGAYYGAQTARAVANFPLSGLRPHPALVRATVVIKKSAARANMTTGRLDPRLGEAIVRAADEALA
GDFDPHFVVDPFQAGAGTSHNMNVNEILANRANELLGGERGAYAPVHPNDHVNMAQSTNDVFPTAMRLASLSLVEKLRPG
LEGLVAALRGKGREFDAVLKSGRTHLQDAVPIRLGQEFEAWAVAMEKNLAGIDAALPGLRELGIGGTAVGTGMNAEAAYI
DLVVAELARETGFPLHRGTNLVERMQNMDPFVALSSALKGLAANLVRIANDLRLLSSGPRTGLAEIALPAIQPGSSIMPG
KVNPSMAEATDMVAFQVMGADAVITLAAQAGQLELNVMMPVIAFNLLFSLEILKNTVPRLVQACIAGITADRERCRRYLD
QSVGLATVLAPYVGYAAAAEVAKESTATGRSIREIVEERQLLTADKLAEILDPLPLTSPGVPGEKHND

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=44.4924406047516, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI87082375, Length=471, Percent_Identity=44.7983014861996, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787896, Length=430, Percent_Identity=46.2790697674419, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=42.8571428571429, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=39.4495412844037, Blast_Score=254, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=44.060475161987, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=42.9805615550756, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=42.9805615550756, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=42.4311926605505, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=39.4396551724138, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24583245, Length=438, Percent_Identity=38.8127853881278, Blast_Score=329, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49679; Mature: 49548

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRIEKDTLGDVQVPAGAYYGAQTARAVANFPLSGLRPHPALVRATVVIKKSAARANMT
CCEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEHHHHHCCCC
TGRLDPRLGEAIVRAADEALAGDFDPHFVVDPFQAGAGTSHNMNVNEILANRANELLGGE
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCC
RGAYAPVHPNDHVNMAQSTNDVFPTAMRLASLSLVEKLRPGLEGLVAALRGKGREFDAVL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
KSGRTHLQDAVPIRLGQEFEAWAVAMEKNLAGIDAALPGLRELGIGGTAVGTGMNAEAAY
HCCCHHHHHCCCHHCCCCHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCEECCCCCCCHHH
IDLVVAELARETGFPLHRGTNLVERMQNMDPFVALSSALKGLAANLVRIANDLRLLSSGP
HHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RTGLAEIALPAIQPGSSIMPGKVNPSMAEATDMVAFQVMGADAVITLAAQAGQLELNVMM
CCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCEEEEEECCCCCEEEHHHH
PVIAFNLLFSLEILKNTVPRLVQACIAGITADRERCRRYLDQSVGLATVLAPYVGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EVAKESTATGRSIREIVEERQLLTADKLAEILDPLPLTSPGVPGEKHND
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFRIEKDTLGDVQVPAGAYYGAQTARAVANFPLSGLRPHPALVRATVVIKKSAARANMT
CEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEHHHHHCCCC
TGRLDPRLGEAIVRAADEALAGDFDPHFVVDPFQAGAGTSHNMNVNEILANRANELLGGE
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCC
RGAYAPVHPNDHVNMAQSTNDVFPTAMRLASLSLVEKLRPGLEGLVAALRGKGREFDAVL
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHH
KSGRTHLQDAVPIRLGQEFEAWAVAMEKNLAGIDAALPGLRELGIGGTAVGTGMNAEAAY
HCCCHHHHHCCCHHCCCCHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCEECCCCCCCHHH
IDLVVAELARETGFPLHRGTNLVERMQNMDPFVALSSALKGLAANLVRIANDLRLLSSGP
HHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RTGLAEIALPAIQPGSSIMPGKVNPSMAEATDMVAFQVMGADAVITLAAQAGQLELNVMM
CCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCEEEEEECCCCCEEEHHHH
PVIAFNLLFSLEILKNTVPRLVQACIAGITADRERCRRYLDQSVGLATVLAPYVGYAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EVAKESTATGRSIREIVEERQLLTADKLAEILDPLPLTSPGVPGEKHND
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]