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The map label for this gene is cheB2

Identifier: 78223507

GI number: 78223507

Start: 2612182

End: 2613264

Strand: Reverse

Name: cheB2

Synonym: Gmet_2304

Alternate gene names: 78223507

Gene position: 2613264-2612182 (Counterclockwise)

Preceding gene: 78223508

Following gene: 78223506

Centisome position: 65.37

GC content: 60.39

Gene sequence:

>1083_bases
ATGAGAAAAATCAGGGTTGTCGTCATCGACGATTCCGCCTTCAACCGGCGGGCCATTATCAAAATGCTTGAAAGCATGCC
CGAGGTGGTGGTGGCGGGATACGCCAACGACGGCGAGGAGGGGATCAGAAAAGTCATTGATCTGAAGCCCGACCTCGTGA
CCCTTGACCTCGAGATGCCCAAGATGGACGGCTTCACTCTCCTGCGGATCATCATGGGGTACTGTCCGACACCGGTCATT
GTGATCAGCGCCAAGAGTGATGACGAGAAGGTCTTCAAGGCTCTTGAACTGGGAGCGGTCGATTTCGTCGCCAAGCCGAG
CCAGGGGATTTCCGAGGAACTCCTTACCATAACCGACGATATCCAGCAGAAGGTGCGGGGCGTCTTTTCCCTGAACATGA
AGGAGATCATCCGCCGTGAGAAAGTGGAGGTTTTGGCTCCCCCTCCTGTCGCCCCGAAAAAGGCGGAAGCGGAGCCGCGA
CGTGTGGTGCCGTGCCGTCTCGATGTGGTGGCCATCGGTTCTTCCACCGGCGGCCCCCCCGCCATCCAGCGGATTCTCTC
TTCGTTCCGCGAGGCCCTTCCCCTGGCGATCGTTATTTCTCAACACATGCCCGCCGGTTTCACGCGAACCTTCGCGGAGC
GCCTCAACCGCCTCTCCGTTTTCGAGGTCAAGGAAGCGGCCGACGGCGATACGGTGTGGCCGGGGCGGGTGCTGATTGCT
CCCGGCGGCCACAACATGGTGTTCGAGAAGAGTTCCGGCTCAGTGGTGGTGAGGGTGCGCAAACCATCTCCCGAGGATCG
CTACATCCCATCGGTCGACGCCATGCTTGCCTCCTGTGCGGAAGTCTTTGGACCGCGGACCCTTGGCGTAGTGCTTACGG
GCATGGGGAACGATGGGAGCCGGGGGGTGCGGGCCATCAAAGGCGCGGGAGGACAAGCCCTTGCCGAAGCCGAGAGTTCG
GCAGTGGTGTTTGGAATGCCGAGGGAGGCCATCGCCACGGGTGTGGTTGATAAGGTGGTACCCCTCGATCTGATTGCGCG
GGAAATCCGTATGCGTTGCGGAGTGGCCACCGACCTTGATTAG

Upstream 100 bases:

>100_bases
CCGAATCGCTCATGAATATCTCCACGGCCTTCACCCTTAAACACTTCAAGAACGACATGGTCTACCAGAAGCCCTTCCCG
GGCGGGGCAGGGGGTGGATT

Downstream 100 bases:

>100_bases
CAGGCCGAGGGTCCGCTGCGTAACCGCCGCGCCGTATAAACAAGTGTGGTGCTGGGAGCAAGTCATGTCGATGGATGACA
AGAGTGATGAAAAAGGGATG

Product: response regulator receiver (CheY-like) modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRKIRVVVIDDSAFNRRAIIKMLESMPEVVVAGYANDGEEGIRKVIDLKPDLVTLDLEMPKMDGFTLLRIIMGYCPTPVI
VISAKSDDEKVFKALELGAVDFVAKPSQGISEELLTITDDIQQKVRGVFSLNMKEIIRREKVEVLAPPPVAPKKAEAEPR
RVVPCRLDVVAIGSSTGGPPAIQRILSSFREALPLAIVISQHMPAGFTRTFAERLNRLSVFEVKEAADGDTVWPGRVLIA
PGGHNMVFEKSSGSVVVRVRKPSPEDRYIPSVDAMLASCAEVFGPRTLGVVLTGMGNDGSRGVRAIKGAGGQALAEAESS
AVVFGMPREAIATGVVDKVVPLDLIAREIRMRCGVATDLD

Sequences:

>Translated_360_residues
MRKIRVVVIDDSAFNRRAIIKMLESMPEVVVAGYANDGEEGIRKVIDLKPDLVTLDLEMPKMDGFTLLRIIMGYCPTPVI
VISAKSDDEKVFKALELGAVDFVAKPSQGISEELLTITDDIQQKVRGVFSLNMKEIIRREKVEVLAPPPVAPKKAEAEPR
RVVPCRLDVVAIGSSTGGPPAIQRILSSFREALPLAIVISQHMPAGFTRTFAERLNRLSVFEVKEAADGDTVWPGRVLIA
PGGHNMVFEKSSGSVVVRVRKPSPEDRYIPSVDAMLASCAEVFGPRTLGVVLTGMGNDGSRGVRAIKGAGGQALAEAESS
AVVFGMPREAIATGVVDKVVPLDLIAREIRMRCGVATDLD
>Mature_360_residues
MRKIRVVVIDDSAFNRRAIIKMLESMPEVVVAGYANDGEEGIRKVIDLKPDLVTLDLEMPKMDGFTLLRIIMGYCPTPVI
VISAKSDDEKVFKALELGAVDFVAKPSQGISEELLTITDDIQQKVRGVFSLNMKEIIRREKVEVLAPPPVAPKKAEAEPR
RVVPCRLDVVAIGSSTGGPPAIQRILSSFREALPLAIVISQHMPAGFTRTFAERLNRLSVFEVKEAADGDTVWPGRVLIA
PGGHNMVFEKSSGSVVVRVRKPSPEDRYIPSVDAMLASCAEVFGPRTLGVVLTGMGNDGSRGVRAIKGAGGQALAEAESS
AVVFGMPREAIATGVVDKVVPLDLIAREIRMRCGVATDLD

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=355, Percent_Identity=40, Blast_Score=237, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_GEOMG (Q39T95)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385254.1
- ProteinModelPortal:   Q39T95
- STRING:   Q39T95
- GeneID:   3740459
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2304
- NMPDR:   fig|269799.3.peg.2548
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   SQHMPAG
- PhylomeDB:   Q39T95
- ProtClustDB:   CLSK828757
- BioCyc:   GMET269799:GMET_2304-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38866; Mature: 38866

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 175-175 ACT_SITE 202-202 ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIRVVVIDDSAFNRRAIIKMLESMPEVVVAGYANDGEEGIRKVIDLKPDLVTLDLEMP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCC
KMDGFTLLRIIMGYCPTPVIVISAKSDDEKVFKALELGAVDFVAKPSQGISEELLTITDD
CCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHH
IQQKVRGVFSLNMKEIIRREKVEVLAPPPVAPKKAEAEPRRVVPCRLDVVAIGSSTGGPP
HHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCEECCEEEEEEEECCCCCCCH
AIQRILSSFREALPLAIVISQHMPAGFTRTFAERLNRLSVFEVKEAADGDTVWPGRVLIA
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCEEEEE
PGGHNMVFEKSSGSVVVRVRKPSPEDRYIPSVDAMLASCAEVFGPRTLGVVLTGMGNDGS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCC
RGVRAIKGAGGQALAEAESSAVVFGMPREAIATGVVDKVVPLDLIAREIRMRCGVATDLD
CCHHHHCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRKIRVVVIDDSAFNRRAIIKMLESMPEVVVAGYANDGEEGIRKVIDLKPDLVTLDLEMP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCC
KMDGFTLLRIIMGYCPTPVIVISAKSDDEKVFKALELGAVDFVAKPSQGISEELLTITDD
CCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHH
IQQKVRGVFSLNMKEIIRREKVEVLAPPPVAPKKAEAEPRRVVPCRLDVVAIGSSTGGPP
HHHHHHHHHCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCEECCEEEEEEEECCCCCCCH
AIQRILSSFREALPLAIVISQHMPAGFTRTFAERLNRLSVFEVKEAADGDTVWPGRVLIA
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEHHCCCCCCCCCCEEEEE
PGGHNMVFEKSSGSVVVRVRKPSPEDRYIPSVDAMLASCAEVFGPRTLGVVLTGMGNDGS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCC
RGVRAIKGAGGQALAEAESSAVVFGMPREAIATGVVDKVVPLDLIAREIRMRCGVATDLD
CCHHHHCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA