Click here to switch to the map view.

The map label for this gene is lexA

Identifier: 78222651

GI number: 78222651

Start: 1608980

End: 1609585

Strand: Direct

Name: lexA

Synonym: Gmet_1439

Alternate gene names: 78222651

Gene position: 1608980-1609585 (Clockwise)

Preceding gene: 78222650

Following gene: 78222652

Centisome position: 40.25

GC content: 62.38

Gene sequence:

>606_bases
ATGGAAGAACTCACCCCTCGCCAGAACGAAGTCCTCCGATTTCTCGAAGGCTACCTCACCCAATACGGCTATCCCCCAAC
CATGAGGGACATTGCCGCCCATCTGCGGATAAGTGGCACCCTTGGGGTATCGAAACACCTCACCGCCTTGGAACGCAAAG
GGTACATCCGCAGGGACCCTGGCAACTCCCGCGGCATTTCCCTCGTGGGCCACGGATCAAAATCGGCATCGCTCCCGATC
GCTGGCGTGGTGCGGGCCGGCATGCTCCAGCCGGCCATCGAGGATATCGAGGGGTATCTGGCCATCGACCAGGCCCAGCT
CAAGGGGGGAAAGTTCTTCCTGCGGGTGAAAGGGGACTCCATGGTGAACGCCGCCATCCTCGACGGCGACCTGGCCCTCA
TCCGTCCGCAGCCCACTGCGGAGAATAACGACATCGTCGTCGCCATGGTCGACGGCGAGGCGACCCTCAAGGCCTTTTAC
CGGGAGCGGGGACAGATTCGCCTCCAGCCCCGCAACCCGAACATGGAGCCGATCATCATCCGGGAAGGGGAGGGTGAGGT
CGCGATCGTCGGCAAGGTGGTCGGGATCTTCCGGACCCTGGAATAA

Upstream 100 bases:

>100_bases
CAAGCTCTTCCAGGGGTAACCTCCCTCACACACCACCCGCTTGACACGCTGCTCCTCTGGTGATAGGTTGGCACATGTGA
ACCTATAGAGGAGCAGCGCC

Downstream 100 bases:

>100_bases
GCCATGCTGCAGCGGGTACAGGAACAGATCAGAGTCGGGGCCGTCTTTGGCCCGGGGCCGTCCATCCACCCGGTCTGGTT
CGACTGGCGGCAACAGAAAC

Product: SOS-response transcriptional repressor, LexA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEELTPRQNEVLRFLEGYLTQYGYPPTMRDIAAHLRISGTLGVSKHLTALERKGYIRRDPGNSRGISLVGHGSKSASLPI
AGVVRAGMLQPAIEDIEGYLAIDQAQLKGGKFFLRVKGDSMVNAAILDGDLALIRPQPTAENNDIVVAMVDGEATLKAFY
RERGQIRLQPRNPNMEPIIIREGEGEVAIVGKVVGIFRTLE

Sequences:

>Translated_201_residues
MEELTPRQNEVLRFLEGYLTQYGYPPTMRDIAAHLRISGTLGVSKHLTALERKGYIRRDPGNSRGISLVGHGSKSASLPI
AGVVRAGMLQPAIEDIEGYLAIDQAQLKGGKFFLRVKGDSMVNAAILDGDLALIRPQPTAENNDIVVAMVDGEATLKAFY
RERGQIRLQPRNPNMEPIIIREGEGEVAIVGKVVGIFRTLE
>Mature_201_residues
MEELTPRQNEVLRFLEGYLTQYGYPPTMRDIAAHLRISGTLGVSKHLTALERKGYIRRDPGNSRGISLVGHGSKSASLPI
AGVVRAGMLQPAIEDIEGYLAIDQAQLKGGKFFLRVKGDSMVNAAILDGDLALIRPQPTAENNDIVVAMVDGEATLKAFY
RERGQIRLQPRNPNMEPIIIREGEGEVAIVGKVVGIFRTLE

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=200, Percent_Identity=35.5, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787431, Length=122, Percent_Identity=31.9672131147541, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_GEOMG (Q39VQ1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384398.1
- ProteinModelPortal:   Q39VQ1
- SMR:   Q39VQ1
- STRING:   Q39VQ1
- MEROPS:   S24.001
- GeneID:   3739082
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1439
- NMPDR:   fig|269799.3.peg.1280
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   Q39VQ1
- ProtClustDB:   CLSK827607
- BioCyc:   GMET269799:GMET_1439-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22021; Mature: 22021

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: ACT_SITE 120-120 ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELTPRQNEVLRFLEGYLTQYGYPPTMRDIAAHLRISGTLGVSKHLTALERKGYIRRDP
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHCCCEEECC
GNSRGISLVGHGSKSASLPIAGVVRAGMLQPAIEDIEGYLAIDQAQLKGGKFFLRVKGDS
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHEECCCCEEEEEECCCC
MVNAAILDGDLALIRPQPTAENNDIVVAMVDGEATLKAFYRERGQIRLQPRNPNMEPIII
EEEEEEECCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCCCCEEEE
REGEGEVAIVGKVVGIFRTLE
ECCCCCEEEEEEHHHHHHCCC
>Mature Secondary Structure
MEELTPRQNEVLRFLEGYLTQYGYPPTMRDIAAHLRISGTLGVSKHLTALERKGYIRRDP
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHCCCEEECC
GNSRGISLVGHGSKSASLPIAGVVRAGMLQPAIEDIEGYLAIDQAQLKGGKFFLRVKGDS
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHEECCCCEEEEEECCCC
MVNAAILDGDLALIRPQPTAENNDIVVAMVDGEATLKAFYRERGQIRLQPRNPNMEPIII
EEEEEEECCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCCCCEEEE
REGEGEVAIVGKVVGIFRTLE
ECCCCCEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA