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The map label for this gene is mdh

Identifier: 78222572

GI number: 78222572

Start: 1524251

End: 1525204

Strand: Direct

Name: mdh

Synonym: Gmet_1360

Alternate gene names: 78222572

Gene position: 1524251-1525204 (Clockwise)

Preceding gene: 78222571

Following gene: 78222573

Centisome position: 38.13

GC content: 54.09

Gene sequence:

>954_bases
ATGGCACGTAAGAAAATCTCTCTCATCGGTGGTGGTCAGATTGGTGGCGTTCTTGCTCAACTTTGCGCCCTGCGCGAACT
TGGCGATGTAGTGATGTTTGATATTGTCGAAGGTCTTCCTCAGGGGAAGATGCTTGATATCGCTGAGGTTGGTCCGGTTG
ACGGTTTTGATGTATGCCTCAAGGGTACCAACAGCTACGCGGATATAGCTGGCTCCGATGTTGTCATCGTAACCGCTGGT
CTTCCCCGCAAGCCGGGCATGAGCCGTGACGATCTCATCGAAGTCAACTCCAAGATCATGACCCAGGTTGCTGAGGGTAT
TAAGCAATATGCCCCCAATTCTTTCGTAATCGTTATCTCCAACCCGCTGGACGCCATGGTTACCCTTTGCCAGAAAATCA
CCGGCTTCCCCTACAACAGGGTTATCGGCCAGGCAGGCGTTCTCGACTCCTCCCGTTTTGCAGCCTTCATCGCCTGGGAA
CTGGGCGTTTCCGTGAAGGATGTCGTTGCCGTAACCCTCGGTGGCCATGGTGATGACATGGTGCCTCTGGTTCGTTATAC
TAGTGTTTGTGGCATCCCGGTCATGGAACTTCTTGAGCGTAAGTATAAGGATAAGGCTAAGGCCAAGGAAGTCATGGAGG
CAATGGTTAAGCGTACTCGCGGTGCGGGTGGTGAGGTCGTCGCTCTCCTCAAGACCGGTTCTGCATTCTACTCTCCGGCC
TCTGCCGCAATCGCCATGACCGAGTCGATCCTCAAGGACCAGAAGCGGGTTCTTCCGACCTGCTGCTTCCTCCAGGGCGA
GTTCGGCGTGAATGGATACTACGTCGGTGTTCCTGCAGTGCTGGGCGAGAACGGTGTTGAGCAGATCATTCAGTTCAACC
TCGACGCCGAAGAGCAGGCAATGATGGATAAGTCGGTTGCCGCGGTGAAGAGTCTCGTCGACAGCCTTAAGTAA

Upstream 100 bases:

>100_bases
AGGCAATGCGTCCTAGCCCGACCCTGAACGCAATTATTGATGCTATCTAAAGGTAGTTGTATCACGCAGATAACCCCAAT
TCAAAACGGAGGAAGAAAAC

Downstream 100 bases:

>100_bases
TTTTTCCGATTCCCACTGCAGCAAGGCAAAAGAAGGGCCCTGTGCCCTTCTTTTGTCATGTCGGGTCTTCCCTTTAAGAA
AATAACAGTATTTTATGATC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARKKISLIGGGQIGGVLAQLCALRELGDVVMFDIVEGLPQGKMLDIAEVGPVDGFDVCLKGTNSYADIAGSDVVIVTAG
LPRKPGMSRDDLIEVNSKIMTQVAEGIKQYAPNSFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSSRFAAFIAWE
LGVSVKDVVAVTLGGHGDDMVPLVRYTSVCGIPVMELLERKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
SAAIAMTESILKDQKRVLPTCCFLQGEFGVNGYYVGVPAVLGENGVEQIIQFNLDAEEQAMMDKSVAAVKSLVDSLK

Sequences:

>Translated_317_residues
MARKKISLIGGGQIGGVLAQLCALRELGDVVMFDIVEGLPQGKMLDIAEVGPVDGFDVCLKGTNSYADIAGSDVVIVTAG
LPRKPGMSRDDLIEVNSKIMTQVAEGIKQYAPNSFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSSRFAAFIAWE
LGVSVKDVVAVTLGGHGDDMVPLVRYTSVCGIPVMELLERKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
SAAIAMTESILKDQKRVLPTCCFLQGEFGVNGYYVGVPAVLGENGVEQIIQFNLDAEEQAMMDKSVAAVKSLVDSLK
>Mature_316_residues
ARKKISLIGGGQIGGVLAQLCALRELGDVVMFDIVEGLPQGKMLDIAEVGPVDGFDVCLKGTNSYADIAGSDVVIVTAGL
PRKPGMSRDDLIEVNSKIMTQVAEGIKQYAPNSFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSSRFAAFIAWEL
GVSVKDVVAVTLGGHGDDMVPLVRYTSVCGIPVMELLERKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPAS
AAIAMTESILKDQKRVLPTCCFLQGEFGVNGYYVGVPAVLGENGVEQIIQFNLDAEEQAMMDKSVAAVKSLVDSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=319, Percent_Identity=32.6018808777429, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI15082234, Length=319, Percent_Identity=29.7805642633229, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI5031857, Length=307, Percent_Identity=30.9446254071661, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI260099723, Length=307, Percent_Identity=30.9446254071661, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=31.023102310231, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=31.023102310231, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI291575128, Length=323, Percent_Identity=28.4829721362229, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI4557032, Length=323, Percent_Identity=28.4829721362229, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=32.4200913242009, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI260099725, Length=211, Percent_Identity=32.2274881516588, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI207028494, Length=191, Percent_Identity=32.4607329842932, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI21735621, Length=254, Percent_Identity=27.1653543307087, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI103472011, Length=324, Percent_Identity=23.4567901234568, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789632, Length=330, Percent_Identity=27.5757575757576, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=283, Percent_Identity=23.6749116607774, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=28.1150159744409, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=305, Percent_Identity=26.8852459016393, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=238, Percent_Identity=27.3109243697479, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=218, Percent_Identity=29.3577981651376, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=27.741935483871, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=29.9065420560748, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24663599, Length=288, Percent_Identity=28.4722222222222, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550422, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24663595, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_GEOMG (Q39VY0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384319.1
- ProteinModelPortal:   Q39VY0
- SMR:   Q39VY0
- STRING:   Q39VY0
- GeneID:   3738696
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1360
- NMPDR:   fig|269799.3.peg.1201
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- PhylomeDB:   Q39VY0
- ProtClustDB:   PRK06223
- BioCyc:   GMET269799:GMET_1360-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33730; Mature: 33599

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKISLIGGGQIGGVLAQLCALRELGDVVMFDIVEGLPQGKMLDIAEVGPVDGFDVCL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHEE
KGTNSYADIAGSDVVIVTAGLPRKPGMSRDDLIEVNSKIMTQVAEGIKQYAPNSFVIVIS
ECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NPLDAMVTLCQKITGFPYNRVIGQAGVLDSSRFAAFIAWELGVSVKDVVAVTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHEEEEHHHCCCHHHEEEEEECCCCCCC
VPLVRYTSVCGIPVMELLERKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCH
SAAIAMTESILKDQKRVLPTCCFLQGEFGVNGYYVGVPAVLGENGVEQIIQFNLDAEEQA
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCEEEECCHHHCCHHHHHHHHCCCCCHHHH
MMDKSVAAVKSLVDSLK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKKISLIGGGQIGGVLAQLCALRELGDVVMFDIVEGLPQGKMLDIAEVGPVDGFDVCL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCHHHEE
KGTNSYADIAGSDVVIVTAGLPRKPGMSRDDLIEVNSKIMTQVAEGIKQYAPNSFVIVIS
ECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NPLDAMVTLCQKITGFPYNRVIGQAGVLDSSRFAAFIAWELGVSVKDVVAVTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHEEEEHHHCCCHHHEEEEEECCCCCCC
VPLVRYTSVCGIPVMELLERKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCH
SAAIAMTESILKDQKRVLPTCCFLQGEFGVNGYYVGVPAVLGENGVEQIIQFNLDAEEQA
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCEEEECCHHHCCHHHHHHHHCCCCCHHHH
MMDKSVAAVKSLVDSLK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA