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The map label for this gene is ilvI [H]

Identifier: 78222474

GI number: 78222474

Start: 1420132

End: 1421832

Strand: Direct

Name: ilvI [H]

Synonym: Gmet_1260

Alternate gene names: 78222474

Gene position: 1420132-1421832 (Clockwise)

Preceding gene: 78222473

Following gene: 78222475

Centisome position: 35.53

GC content: 58.55

Gene sequence:

>1701_bases
ATGAAAATGACAGGTGCACGAATCCTCCTTGAATGTCTCAAGCGTGAAGGAGTTGATACTGTTTTCGGTTACCCCGGGGG
CACGGTGATCAATCTCTACGACGAGCTTTTCAGCTTCAAGGATATCCGCCATATTCTGCCCCGCCACGAACAGGCAGGTG
TCCATGCGGCTGATGGATTTGCCCGTGCCACCGGCAGGGTCGGTGTCGCCATCGCCACCTCCGGCCCCGGTGCCACCAAT
ACGGTTACCGGTATCGCGACCGCCTACATGGACTCCATCCCCCTTGTGGTGATAACCGGCCAGGTGCCCACGGCCCTCAT
TGGCAATGATGCCTTCCAGGAGGTGGACATCATCGGCATTACCCGCCCCTGCACCAAGCACAACTTTCTCGTGAAAGACG
TGAAGGATATCGCCACGATCATCAAGAAAGCCTTTTACATTGCCCGCACCGGGCGTCCCGGGCCGGTTCTCGTGGACCTG
CCGAAGGATGTGCAGATCGCCACGGCCGAGTTTGAGTATCCCGAAGCCATTGAGCTTCGCAGCTACAAACCGACCGTGGA
GGGTCACCCGAAGCAGATCGAGAAGGCCGTGTCCATGCTCCTGGCGGCTAAGAAGCCGGTCATGTACGTGGGAGGGGGCG
CCATTTCCGGTGATGCCGCCGGTGAACTCACTGCCCTAGCCACCAGACTCCACATCCCGGTCACCACAACCCTCATGGGA
CTCGGTTCCTTCCCCGAGGACAATCCCCTCTCTCTCAAGCTTCTCGGGATGCATGGCACCTATTTCGCCAATATGGCGGT
GACCAACTGCGACCTGCTGGTTGCCGTGGGGGCGCGCTTCGACGACCGGGTGACCGGAAAGATCGCCACCTTCGCTCCCC
ATGCAAAGATCATCCATATGGACGTGGACCCGACATCCATTCGGAAGAACGTCCGGGTTGACCTTCCGATTGTTGGTCAG
GTCAAGGAAGTCCTTGGTCGCATGCTCAAGTCCCTTGATGAGAGCGGTGACAAGGTAGCCGCGTTCCATACGGCGATCGA
ACCGTGGCTTGCGGAAATCGAGGGGTGGAAGCAGAAGCATCCAATAACCTACAAGCAGGCATCCACGGTGATCAAACCCC
AGTTCGTGATCCAGAAACTTCGTGAGCTTTCTGATCCCGACGCCATTGTGGCCACCGACGTGGGGCAGCACCAGATGTGG
ACTGCCCAGTTCTTCGGGTTCCGTGAGCCCCGTACGCTCCTGTCGTCCGGCGGGCTCGGAACCATGGGATTCGGATTGCC
AGCGGCCATGGGGGCCCAGGCAGCCTTCCCGAACCGGCAGGTCATCGCGGTTTGCGGCGACGGCGGTTTCCAGATGAACC
TCCAGGAGGTGGCAACGCTGGTCCAGAATCGGCTTCCCGTGAAGATCGTCATACTCAACAATAACTTCCTCGGCATGGTG
CGCCAGTGGCAGGAACTCTTCTTCGATAAGCGCTATTCACAGACCTGCATGGAGCTTCCCATTGATTTCATCAAGCTTGC
CGAGGCGTTCGGGGCTAAGGGGTTCCAGGCGACAAAATCCGACGAGGTGGAAGCTACCATCAAGAAGGCCTTCGAGACGC
CAGGACCGGTTATCATGGAATTCAAGATCGCCCGGGAGGAGAAGGTTCTCCCCATGGTTCCTGCCGGGGCGTCGCTCAAT
GAAATGGTGCTCAATGCCTGA

Upstream 100 bases:

>100_bases
TACACCGGCGCGGTTACCACAGCCGAATAGGAACGATTCTGCAATCGCTCCCGTAGACCAGTCGCTCCACTATCTGATTT
CCCTTTTGGGAGGTTTTTCT

Downstream 100 bases:

>100_bases
TTGGCGCCGTTACGCGCCAGGGATTCCGATCTTGCCTGCTCTTCAGGAGGTTTTTTTATGAGACATACAATCACCGTTCT
GGTGGAAAACGAATTTGGTG

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKMTGARILLECLKREGVDTVFGYPGGTVINLYDELFSFKDIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPLVVITGQVPTALIGNDAFQEVDIIGITRPCTKHNFLVKDVKDIATIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFEYPEAIELRSYKPTVEGHPKQIEKAVSMLLAAKKPVMYVGGGAISGDAAGELTALATRLHIPVTTTLMG
LGSFPEDNPLSLKLLGMHGTYFANMAVTNCDLLVAVGARFDDRVTGKIATFAPHAKIIHMDVDPTSIRKNVRVDLPIVGQ
VKEVLGRMLKSLDESGDKVAAFHTAIEPWLAEIEGWKQKHPITYKQASTVIKPQFVIQKLRELSDPDAIVATDVGQHQMW
TAQFFGFREPRTLLSSGGLGTMGFGLPAAMGAQAAFPNRQVIAVCGDGGFQMNLQEVATLVQNRLPVKIVILNNNFLGMV
RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKSDEVEATIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA

Sequences:

>Translated_566_residues
MKMTGARILLECLKREGVDTVFGYPGGTVINLYDELFSFKDIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPLVVITGQVPTALIGNDAFQEVDIIGITRPCTKHNFLVKDVKDIATIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFEYPEAIELRSYKPTVEGHPKQIEKAVSMLLAAKKPVMYVGGGAISGDAAGELTALATRLHIPVTTTLMG
LGSFPEDNPLSLKLLGMHGTYFANMAVTNCDLLVAVGARFDDRVTGKIATFAPHAKIIHMDVDPTSIRKNVRVDLPIVGQ
VKEVLGRMLKSLDESGDKVAAFHTAIEPWLAEIEGWKQKHPITYKQASTVIKPQFVIQKLRELSDPDAIVATDVGQHQMW
TAQFFGFREPRTLLSSGGLGTMGFGLPAAMGAQAAFPNRQVIAVCGDGGFQMNLQEVATLVQNRLPVKIVILNNNFLGMV
RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKSDEVEATIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA
>Mature_566_residues
MKMTGARILLECLKREGVDTVFGYPGGTVINLYDELFSFKDIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPLVVITGQVPTALIGNDAFQEVDIIGITRPCTKHNFLVKDVKDIATIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFEYPEAIELRSYKPTVEGHPKQIEKAVSMLLAAKKPVMYVGGGAISGDAAGELTALATRLHIPVTTTLMG
LGSFPEDNPLSLKLLGMHGTYFANMAVTNCDLLVAVGARFDDRVTGKIATFAPHAKIIHMDVDPTSIRKNVRVDLPIVGQ
VKEVLGRMLKSLDESGDKVAAFHTAIEPWLAEIEGWKQKHPITYKQASTVIKPQFVIQKLRELSDPDAIVATDVGQHQMW
TAQFFGFREPRTLLSSGGLGTMGFGLPAAMGAQAAFPNRQVIAVCGDGGFQMNLQEVATLVQNRLPVKIVILNNNFLGMV
RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKSDEVEATIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=551, Percent_Identity=25.7713248638838, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=25.6227758007117, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=49.8239436619718, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=42.9840142095915, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=34.2960288808664, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=30.2702702702703, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=26.6429840142096, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=585, Percent_Identity=28.8888888888889, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=585, Percent_Identity=28.8888888888889, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=516, Percent_Identity=27.7131782945736, Blast_Score=148, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=46.8267581475129, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320816, Length=491, Percent_Identity=25.6619144602851, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320123, Length=551, Percent_Identity=23.2304900181488, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=540, Percent_Identity=23.7037037037037, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=594, Percent_Identity=25.0841750841751, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323073, Length=594, Percent_Identity=25.2525252525253, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=25.7839721254355, Blast_Score=170, Evalue=3e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61485; Mature: 61485

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTGARILLECLKREGVDTVFGYPGGTVINLYDELFSFKDIRHILPRHEQAGVHAADGF
CCCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCH
ARATGRVGVAIATSGPGATNTVTGIATAYMDSIPLVVITGQVPTALIGNDAFQEVDIIGI
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCHHCEEEEEE
TRPCTKHNFLVKDVKDIATIIKKAFYIARTGRPGPVLVDLPKDVQIATAEFEYPEAIELR
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEEECCCCCCCEEEC
SYKPTVEGHPKQIEKAVSMLLAAKKPVMYVGGGAISGDAAGELTALATRLHIPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCHHHHHHHEEECCHHHHHHH
LGSFPEDNPLSLKLLGMHGTYFANMAVTNCDLLVAVGARFDDRVTGKIATFAPHAKIIHM
CCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCEEEEE
DVDPTSIRKNVRVDLPIVGQVKEVLGRMLKSLDESGDKVAAFHTAIEPWLAEIEGWKQKH
ECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHCC
PITYKQASTVIKPQFVIQKLRELSDPDAIVATDVGQHQMWTAQFFGFREPRTLLSSGGLG
CEEHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHCCCCCCHHHHHCCCCC
TMGFGLPAAMGAQAAFPNRQVIAVCGDGGFQMNLQEVATLVQNRLPVKIVILNNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKSDEVEATIKKAFETPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
FKIAREEKVLPMVPAGASLNEMVLNA
EEECCCCCCCEECCCCCCHHHHHCCC
>Mature Secondary Structure
MKMTGARILLECLKREGVDTVFGYPGGTVINLYDELFSFKDIRHILPRHEQAGVHAADGF
CCCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCH
ARATGRVGVAIATSGPGATNTVTGIATAYMDSIPLVVITGQVPTALIGNDAFQEVDIIGI
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCHHCEEEEEE
TRPCTKHNFLVKDVKDIATIIKKAFYIARTGRPGPVLVDLPKDVQIATAEFEYPEAIELR
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEEECCCCCCCEEEC
SYKPTVEGHPKQIEKAVSMLLAAKKPVMYVGGGAISGDAAGELTALATRLHIPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCHHHHHHHEEECCHHHHHHH
LGSFPEDNPLSLKLLGMHGTYFANMAVTNCDLLVAVGARFDDRVTGKIATFAPHAKIIHM
CCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCEEEEE
DVDPTSIRKNVRVDLPIVGQVKEVLGRMLKSLDESGDKVAAFHTAIEPWLAEIEGWKQKH
ECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHCC
PITYKQASTVIKPQFVIQKLRELSDPDAIVATDVGQHQMWTAQFFGFREPRTLLSSGGLG
CEEHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHCCCCCCHHHHHCCCCC
TMGFGLPAAMGAQAAFPNRQVIAVCGDGGFQMNLQEVATLVQNRLPVKIVILNNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDKRYSQTCMELPIDFIKLAEAFGAKGFQATKSDEVEATIKKAFETPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
FKIAREEKVLPMVPAGASLNEMVLNA
EEECCCCCCCEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]