Click here to switch to the map view.

The map label for this gene is yciL [C]

Identifier: 78222432

GI number: 78222432

Start: 1370838

End: 1371614

Strand: Direct

Name: yciL [C]

Synonym: Gmet_1217

Alternate gene names: 78222432

Gene position: 1370838-1371614 (Clockwise)

Preceding gene: 78222431

Following gene: 78222433

Centisome position: 34.29

GC content: 66.02

Gene sequence:

>777_bases
ATGGAAGAACGGCTGCAGAAAATACTCTCCCAGGCCGGCATCGCCTCCCGCCGGGAGGCTGAACGGATGATCACCGACGG
GCGCGTGGCGGTGAACGGCAAGCCGGTCATCGAGCTTGGCGCCAAGGCTGACTCCTCCCGCGACGCTATCACTGTTGACG
GCAAGCCGGTCACCGTGGACGAGAAGCGGGTCTACATCCTTCTCAACAAGCCGGTGGGGTATGTGACCACCCTCAAGGAC
CCCGAGGGACGTCCCATTGTCACCGAGCTCCTGAAGGGACTCGGCGTTCGGGTTTTCCCCGTGGGACGGCTCGACTACAA
CACGGAGGGGCTCCTTCTCCTCACCAACGACGGGGAATGGGCCAACCGCCTTGCCCATCCCCGCCACGAGGTGGACAAGG
AGTATCTCGTCCGGGTGAGGGGGACGGTCGCCCGGGAGCAGATCACCCGCCTGGAGCAGGGGATTGAGTTGGAGGACGGC
AAGGCCGCGCCGGCACGGGTTTCGGTGACCAAGCAGAGCGACAACAATACCTGGGTTTCCATCACCATCCACGAGGGGCG
CTACCGGCAGGTCCGCCGGATGTGCGAGGCAGTCAGCCTCTCCGTGGTGCGGCTCCGGCGGGTGCGCTACGGCGCCCTCT
CCATGGGAGACCTGAAGATCGGCGCGTATCGGCATCTCACGTCCGGAGAAGTGGCTGCGCTGTCGGACGGGAAGATCGGC
GCACGCGGTGGGGCGCCGCGGCGCCGGGGGCCTTCTGCTCCGCCACGGAAAGGATAA

Upstream 100 bases:

>100_bases
TAGGCACCTCAGGCAGATCGACTGGGCAAAGGCTAGCGGTGAGGGGAGAAAATACTCAGATCCCTCGCCCCTGGCCTTTC
GCCTTTTTCAGGAGACATGC

Downstream 100 bases:

>100_bases
CTGTGTAGCGCCGTCGTAACCAGTGTAACGATTCGCGGACAGGGGTGCACATTTTGTGGGCATTATTACATGGCTGTGAC
GAAGAGTGCTTCGGCACGCA

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MEERLQKILSQAGIASRREAERMITDGRVAVNGKPVIELGAKADSSRDAITVDGKPVTVDEKRVYILLNKPVGYVTTLKD
PEGRPIVTELLKGLGVRVFPVGRLDYNTEGLLLLTNDGEWANRLAHPRHEVDKEYLVRVRGTVAREQITRLEQGIELEDG
KAAPARVSVTKQSDNNTWVSITIHEGRYRQVRRMCEAVSLSVVRLRRVRYGALSMGDLKIGAYRHLTSGEVAALSDGKIG
ARGGAPRRRGPSAPPRKG

Sequences:

>Translated_258_residues
MEERLQKILSQAGIASRREAERMITDGRVAVNGKPVIELGAKADSSRDAITVDGKPVTVDEKRVYILLNKPVGYVTTLKD
PEGRPIVTELLKGLGVRVFPVGRLDYNTEGLLLLTNDGEWANRLAHPRHEVDKEYLVRVRGTVAREQITRLEQGIELEDG
KAAPARVSVTKQSDNNTWVSITIHEGRYRQVRRMCEAVSLSVVRLRRVRYGALSMGDLKIGAYRHLTSGEVAALSDGKIG
ARGGAPRRRGPSAPPRKG
>Mature_258_residues
MEERLQKILSQAGIASRREAERMITDGRVAVNGKPVIELGAKADSSRDAITVDGKPVTVDEKRVYILLNKPVGYVTTLKD
PEGRPIVTELLKGLGVRVFPVGRLDYNTEGLLLLTNDGEWANRLAHPRHEVDKEYLVRVRGTVAREQITRLEQGIELEDG
KAAPARVSVTKQSDNNTWVSITIHEGRYRQVRRMCEAVSLSVVRLRRVRYGALSMGDLKIGAYRHLTSGEVAALSDGKIG
ARGGAPRRRGPSAPPRKG

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=221, Percent_Identity=46.1538461538462, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=37.1794871794872, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=35.1931330472103, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=34.4632768361582, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28463; Mature: 28463

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKILSQAGIASRREAERMITDGRVAVNGKPVIELGAKADSSRDAITVDGKPVTVD
CHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCEEEECCEEEEEC
EKRVYILLNKPVGYVTTLKDPEGRPIVTELLKGLGVRVFPVGRLDYNTEGLLLLTNDGEW
CCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCHH
ANRLAHPRHEVDKEYLVRVRGTVAREQITRLEQGIELEDGKAAPARVSVTKQSDNNTWVS
HHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEECCCCEEEE
ITIHEGRYRQVRRMCEAVSLSVVRLRRVRYGALSMGDLKIGAYRHLTSGEVAALSDGKIG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEECEEEECCCCCEEEECCCCCC
ARGGAPRRRGPSAPPRKG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEERLQKILSQAGIASRREAERMITDGRVAVNGKPVIELGAKADSSRDAITVDGKPVTVD
CHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEECCCCCCCCCEEEECCEEEEEC
EKRVYILLNKPVGYVTTLKDPEGRPIVTELLKGLGVRVFPVGRLDYNTEGLLLLTNDGEW
CCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCHH
ANRLAHPRHEVDKEYLVRVRGTVAREQITRLEQGIELEDGKAAPARVSVTKQSDNNTWVS
HHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEECCCCEEEE
ITIHEGRYRQVRRMCEAVSLSVVRLRRVRYGALSMGDLKIGAYRHLTSGEVAALSDGKIG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEECEEEECCCCCEEEECCCCCC
ARGGAPRRRGPSAPPRKG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]