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The map label for this gene is hflB [C]

Identifier: 78222349

GI number: 78222349

Start: 1254546

End: 1255883

Strand: Direct

Name: hflB [C]

Synonym: Gmet_1133

Alternate gene names: 78222349

Gene position: 1254546-1255883 (Clockwise)

Preceding gene: 78222348

Following gene: 78222350

Centisome position: 31.38

GC content: 70.93

Gene sequence:

>1338_bases
ATGGAAGCCGCCGCACGGACCATGACAGAGGATACCTTCTCCCCCGAGTGGGAGCGGATCGGCCTCCTGGCCCAGATCCT
GGCCGGGTCCCGCCAGGGGCGGGAGGCGTCAACGGGGGAGCTGGAGCGGCTCCGGACGCTCCAGCAGCAGGTGGAGGCGT
CCCGCCGGCAAGAGGGGGGCTGGCGTGTGGTGGCACCGGCGGGGCTCTCTCCCATCGAGTTCGACGTCCTCGCCTGCGTC
GTGGCCCCCGAGGCGGAGCCCCGACTTGGGTGGCTCTTCCAGACTCTTCAGCCCGGCACCCCCACCCCCTACCCGACGCC
GGCGCTACTCCAGGAGCTTCTGGCCATGGCCCCGGAGGAGACGCCGCTCCTCTACGGTGCCCTCACCGAGTCATCCCCCC
TGCGCATCGGCGGCCTCATCGAGGTGGAGGGGGGGGATCCGTTCCAACCGGTGAAACCGGTACCCGGCGTAACGGCGAGG
CTCCTGGGGCGTCCCGCCGTCACCGGCGATCTCCCAGGGGCGGTATTGGTGAAAACCCCGGCCCGCTGGGACGATCTGGT
GCTCCCCCTGGATCGGGTCGCCATGCTCCGGGAGTTTCTCCTCTGGATCACCCACAAGGCCACGGTGGTCGGCGAGTGGG
GAGGCGGCGACTGCGGTGGGCCGGTAGGGCTCTTCGCCGGGCCGTCGGGGACTGGCAAGACCTTCGCCGCGGCGGTCCTG
GCCGCGGAACTGGGGTGGCCCCTCTACCGGGTGGACCTGGGGCGGCTCGTGAGCAAGTACATCGGCGAGACGGAAAAGAA
CCTGAACCGCCTCTTCGACGCGGCCCACGGCCAGCCCATGATCCTCCAGTTCGACGAGGCCGACAGCCTCTTCAGCAAGC
GGGGGGAGGTGCGGGAGGCCCGGGACCGCTACGCCAACATGGAGGTGAGCCACCTCCTGGCACGCATCGAGTCCCACCAG
GGGCCGGTGATCCTCACCACCAATCTCCGCAAGCACCTGGACCCGGCCTTTGCCCGCCGCTTCCAGGTGGTGGTGGACTT
TCCCCGCCCCGATGCCGATGCCCGCACACGGCTCTGGCAGCGGCTCCTCCCCCCCCGGGCACCCTTAGAGCCGGGAGTGG
ACCCTGCCTTCCTCGGGGCCGCCGTCAACCTGACCGGCGGGAGCATCCGCAATGCCGCTCTCCACGCCGCCTACCTGGCC
GCCGGCGCCGGGAAGCCCCTGGGGCTCGGCCACTTGGCCCTGGCGGTCTGGCGGGAACTGGGGAAGGACGGGAGGGAACT
TTCCCCACTCGACCTGGGGCCCCTGGCTCCGTTCCTTCCCAGGGAGGCCCCATGCTGA

Upstream 100 bases:

>100_bases
CGGTTCTCTACGCCGAGCGGAGCTTCGTGCGGGGTGCGGACGGGGTGACAGTGACTGTGCGGAGCAATCCCCTCCTCGTC
ACCGTCCATGGGGGAGCGTG

Downstream 100 bases:

>100_bases
CTATTGATCGCCTGCGCCTTCAATTGCCCCCCGCCTTCCGGGACCGGGCCGGGGAGATCGCCCAACTGGTCGCGGAGGAG
TTGGCAACGGTCCCCATGGC

Product: AAA ATPase, central region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEAAARTMTEDTFSPEWERIGLLAQILAGSRQGREASTGELERLRTLQQQVEASRRQEGGWRVVAPAGLSPIEFDVLACV
VAPEAEPRLGWLFQTLQPGTPTPYPTPALLQELLAMAPEETPLLYGALTESSPLRIGGLIEVEGGDPFQPVKPVPGVTAR
LLGRPAVTGDLPGAVLVKTPARWDDLVLPLDRVAMLREFLLWITHKATVVGEWGGGDCGGPVGLFAGPSGTGKTFAAAVL
AAELGWPLYRVDLGRLVSKYIGETEKNLNRLFDAAHGQPMILQFDEADSLFSKRGEVREARDRYANMEVSHLLARIESHQ
GPVILTTNLRKHLDPAFARRFQVVVDFPRPDADARTRLWQRLLPPRAPLEPGVDPAFLGAAVNLTGGSIRNAALHAAYLA
AGAGKPLGLGHLALAVWRELGKDGRELSPLDLGPLAPFLPREAPC

Sequences:

>Translated_445_residues
MEAAARTMTEDTFSPEWERIGLLAQILAGSRQGREASTGELERLRTLQQQVEASRRQEGGWRVVAPAGLSPIEFDVLACV
VAPEAEPRLGWLFQTLQPGTPTPYPTPALLQELLAMAPEETPLLYGALTESSPLRIGGLIEVEGGDPFQPVKPVPGVTAR
LLGRPAVTGDLPGAVLVKTPARWDDLVLPLDRVAMLREFLLWITHKATVVGEWGGGDCGGPVGLFAGPSGTGKTFAAAVL
AAELGWPLYRVDLGRLVSKYIGETEKNLNRLFDAAHGQPMILQFDEADSLFSKRGEVREARDRYANMEVSHLLARIESHQ
GPVILTTNLRKHLDPAFARRFQVVVDFPRPDADARTRLWQRLLPPRAPLEPGVDPAFLGAAVNLTGGSIRNAALHAAYLA
AGAGKPLGLGHLALAVWRELGKDGRELSPLDLGPLAPFLPREAPC
>Mature_445_residues
MEAAARTMTEDTFSPEWERIGLLAQILAGSRQGREASTGELERLRTLQQQVEASRRQEGGWRVVAPAGLSPIEFDVLACV
VAPEAEPRLGWLFQTLQPGTPTPYPTPALLQELLAMAPEETPLLYGALTESSPLRIGGLIEVEGGDPFQPVKPVPGVTAR
LLGRPAVTGDLPGAVLVKTPARWDDLVLPLDRVAMLREFLLWITHKATVVGEWGGGDCGGPVGLFAGPSGTGKTFAAAVL
AAELGWPLYRVDLGRLVSKYIGETEKNLNRLFDAAHGQPMILQFDEADSLFSKRGEVREARDRYANMEVSHLLARIESHQ
GPVILTTNLRKHLDPAFARRFQVVVDFPRPDADARTRLWQRLLPPRAPLEPGVDPAFLGAAVNLTGGSIRNAALHAAYLA
AGAGKPLGLGHLALAVWRELGKDGRELSPLDLGPLAPFLPREAPC

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Homo sapiens, GI31742536, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI112789543, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI21327685, Length=192, Percent_Identity=30.2083333333333, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI7657689, Length=192, Percent_Identity=30.2083333333333, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4505725, Length=192, Percent_Identity=33.8541666666667, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI24430151, Length=185, Percent_Identity=29.1891891891892, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI195539395, Length=219, Percent_Identity=26.027397260274, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI153792536, Length=194, Percent_Identity=34.5360824742268, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI5901990, Length=250, Percent_Identity=28.4, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI7019569, Length=199, Percent_Identity=31.6582914572864, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI6005942, Length=185, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI14149767, Length=246, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI62177112, Length=246, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789568, Length=192, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17554264, Length=186, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71987372, Length=223, Percent_Identity=26.457399103139, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71987364, Length=223, Percent_Identity=26.457399103139, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17563250, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532375, Length=185, Percent_Identity=29.7297297297297, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI86561515, Length=199, Percent_Identity=30.6532663316583, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554786, Length=185, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6325431, Length=140, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320077, Length=230, Percent_Identity=29.5652173913043, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321709, Length=149, Percent_Identity=37.5838926174497, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324000, Length=143, Percent_Identity=35.6643356643357, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324833, Length=185, Percent_Identity=28.6486486486486, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321390, Length=185, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6325281, Length=235, Percent_Identity=28.936170212766, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21355121, Length=229, Percent_Identity=34.4978165938865, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24639486, Length=198, Percent_Identity=34.8484848484849, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640100, Length=220, Percent_Identity=26.8181818181818, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281360146, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360138, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360142, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357333, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360136, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644411, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281360144, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281360140, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644145, Length=282, Percent_Identity=27.3049645390071, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24581396, Length=144, Percent_Identity=36.8055555555556, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24649446, Length=185, Percent_Identity=28.6486486486486, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 48081; Mature: 48081

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAAARTMTEDTFSPEWERIGLLAQILAGSRQGREASTGELERLRTLQQQVEASRRQEGG
CCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
WRVVAPAGLSPIEFDVLACVVAPEAEPRLGWLFQTLQPGTPTPYPTPALLQELLAMAPEE
EEEEECCCCCCCHHEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
TPLLYGALTESSPLRIGGLIEVEGGDPFQPVKPVPGVTARLLGRPAVTGDLPGAVLVKTP
CCEEEEEECCCCCEEECCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECC
ARWDDLVLPLDRVAMLREFLLWITHKATVVGEWGGGDCGGPVGLFAGPSGTGKTFAAAVL
CCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHH
AAELGWPLYRVDLGRLVSKYIGETEKNLNRLFDAAHGQPMILQFDEADSLFSKRGEVREA
HHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHH
RDRYANMEVSHLLARIESHQGPVILTTNLRKHLDPAFARRFQVVVDFPRPDADARTRLWQ
HHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHCCHHHHHHEEEEEECCCCCHHHHHHHHH
RLLPPRAPLEPGVDPAFLGAAVNLTGGSIRNAALHAAYLAAGAGKPLGLGHLALAVWREL
HHCCCCCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
GKDGRELSPLDLGPLAPFLPREAPC
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAAARTMTEDTFSPEWERIGLLAQILAGSRQGREASTGELERLRTLQQQVEASRRQEGG
CCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
WRVVAPAGLSPIEFDVLACVVAPEAEPRLGWLFQTLQPGTPTPYPTPALLQELLAMAPEE
EEEEECCCCCCCHHEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
TPLLYGALTESSPLRIGGLIEVEGGDPFQPVKPVPGVTARLLGRPAVTGDLPGAVLVKTP
CCEEEEEECCCCCEEECCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECC
ARWDDLVLPLDRVAMLREFLLWITHKATVVGEWGGGDCGGPVGLFAGPSGTGKTFAAAVL
CCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHH
AAELGWPLYRVDLGRLVSKYIGETEKNLNRLFDAAHGQPMILQFDEADSLFSKRGEVREA
HHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHH
RDRYANMEVSHLLARIESHQGPVILTTNLRKHLDPAFARRFQVVVDFPRPDADARTRLWQ
HHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHCCHHHHHHEEEEEECCCCCHHHHHHHHH
RLLPPRAPLEPGVDPAFLGAAVNLTGGSIRNAALHAAYLAAGAGKPLGLGHLALAVWREL
HHCCCCCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
GKDGRELSPLDLGPLAPFLPREAPC
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]