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The map label for this gene is nuoE [H]

Identifier: 78222327

GI number: 78222327

Start: 1231882

End: 1232499

Strand: Direct

Name: nuoE [H]

Synonym: Gmet_1109

Alternate gene names: 78222327

Gene position: 1231882-1232499 (Clockwise)

Preceding gene: 78222326

Following gene: 78222328

Centisome position: 30.82

GC content: 63.43

Gene sequence:

>618_bases
ATGGGACGCACCAGCCGCTTTACCCTCCAATCATCGGAAATCACCCCCGAGAGCATCTCTCAGACCCCCTCTACTCCTAC
AGAATTGACATCCGATCCCCGTTTCCAGGCGGTGGAACGGACCATCAAGCAGTTCCAGTGCCGGGCCGACGCCCTCATTG
AGGTGCTCCACGTGGCCCAGGAGGCCTTCGGCTACCTGAGCGACGAGCTCATGGCCCACGTGGCGAGGCAGTTGAAAATC
CCCTTCAGCCAGGTCTACGGGGTCGCCACCTTCTACCACTTCTTTTCCCTTGAACCCCGCGGCGCCCATACCTGCGTCGT
CTGCACCGGCACGGCCTGCTATGTGAAGCGATCCGCCGAGATCCTTGTGCGCCTTGAGCAGGAGTTCAGGGTCAAGGCCG
GCAAGACCACCGAAGACGGTGGCCTCACGCTCTCCACAATCCGGTGCCTTGGTAGTTGCGGCCAGGCGCCAGTCATGGTT
CTTGACGGGGAGACGGTGGGACAATGCACCCCCGATTCGGCCGTTGTCGCGGTAAAGGCGCTTCAGGAGAGCGGCCGGCC
CCGGCCGAACGATGAGGGAGGGGTGCGGCAGGAGGGGGGAGGGGGGAGAACCCCATGA

Upstream 100 bases:

>100_bases
CGCAGATCGAGAGCCTGGAATGGGGATACTGACGAAGTAAAGTAAACAGAGTTGTTTTTGCCGGAGCCCGTGGTTTACTT
CTGAAGAGGAGGTAAGACCC

Downstream 100 bases:

>100_bases
ACAGGGCTGATCTCAGGCATATGGCCGAGGCGGAGCAGGAGCGTCAGGAGCGGTTCCGTTGCCGGATCATGGTCTGCGCC
GGTACGCCGTGCCTGTCGGC

Product: bidirectional hydrogenase complex protein HoxE

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MGRTSRFTLQSSEITPESISQTPSTPTELTSDPRFQAVERTIKQFQCRADALIEVLHVAQEAFGYLSDELMAHVARQLKI
PFSQVYGVATFYHFFSLEPRGAHTCVVCTGTACYVKRSAEILVRLEQEFRVKAGKTTEDGGLTLSTIRCLGSCGQAPVMV
LDGETVGQCTPDSAVVAVKALQESGRPRPNDEGGVRQEGGGGRTP

Sequences:

>Translated_205_residues
MGRTSRFTLQSSEITPESISQTPSTPTELTSDPRFQAVERTIKQFQCRADALIEVLHVAQEAFGYLSDELMAHVARQLKI
PFSQVYGVATFYHFFSLEPRGAHTCVVCTGTACYVKRSAEILVRLEQEFRVKAGKTTEDGGLTLSTIRCLGSCGQAPVMV
LDGETVGQCTPDSAVVAVKALQESGRPRPNDEGGVRQEGGGGRTP
>Mature_204_residues
GRTSRFTLQSSEITPESISQTPSTPTELTSDPRFQAVERTIKQFQCRADALIEVLHVAQEAFGYLSDELMAHVARQLKIP
FSQVYGVATFYHFFSLEPRGAHTCVVCTGTACYVKRSAEILVRLEQEFRVKAGKTTEDGGLTLSTIRCLGSCGQAPVMVL
DGETVGQCTPDSAVVAVKALQESGRPRPNDEGGVRQEGGGGRTP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=182, Percent_Identity=31.3186813186813, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788621, Length=154, Percent_Identity=33.1168831168831, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17561328, Length=140, Percent_Identity=33.5714285714286, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859877, Length=152, Percent_Identity=34.2105263157895, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21355353, Length=114, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 22213; Mature: 22082

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTSRFTLQSSEITPESISQTPSTPTELTSDPRFQAVERTIKQFQCRADALIEVLHVAQ
CCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFGYLSDELMAHVARQLKIPFSQVYGVATFYHFFSLEPRGAHTCVVCTGTACYVKRSAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ILVRLEQEFRVKAGKTTEDGGLTLSTIRCLGSCGQAPVMVLDGETVGQCTPDSAVVAVKA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHH
LQESGRPRPNDEGGVRQEGGGGRTP
HHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRTSRFTLQSSEITPESISQTPSTPTELTSDPRFQAVERTIKQFQCRADALIEVLHVAQ
CCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFGYLSDELMAHVARQLKIPFSQVYGVATFYHFFSLEPRGAHTCVVCTGTACYVKRSAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ILVRLEQEFRVKAGKTTEDGGLTLSTIRCLGSCGQAPVMVLDGETVGQCTPDSAVVAVKA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHH
LQESGRPRPNDEGGVRQEGGGGRTP
HHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]