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The map label for this gene is cca [H]

Identifier: 78222325

GI number: 78222325

Start: 1230855

End: 1231463

Strand: Direct

Name: cca [H]

Synonym: Gmet_1107

Alternate gene names: 78222325

Gene position: 1230855-1231463 (Clockwise)

Preceding gene: 78222324

Following gene: 78222326

Centisome position: 30.79

GC content: 67.82

Gene sequence:

>609_bases
ATGACCGCCGGGGGGCTCCATAACGGCAGCGGGGGGATGGAATCCGCCCGTCACTGCCGGGAGCTGGTCGAGCGGTGGCT
CGCTGACGGGAGCTTCGATGCGCGGCTCCCTGAAGTGGTCCGACTGCGCGGCGTTCTCCAGCCCGAGCAGTACCATGCCG
AGGGGGACGCCTGGACCCACACCATGCTCGCCATGGAAGCCGTCCCTGACGACGCCGACCCCCGCGTCTTCTGGGGGGTG
CTCCTCCACGATATCGGAAAGGCCGAAACCACCGCCTTCATCAAGGGACGGTGGCGCTCCTGGGGGCATGCGGAAGCCGG
GGCCGGGGAGGTGTCTGCCATCATGACGCGGCTCGGCTTCCCGCACCTTGCTGCCGACGTGACGTGGCTCGTCAAGCATC
ACACCTTCCACTTCTCTTGGAACCTGACCCCCGGCGCACGGCTTTCGCCCAAGCAGCGCCGGTTCATGGAGCACCCCCTC
TTTCCGCTCCTTCTGGAGGTCTGCGACGCCGACGCCGCCGGCTCCCGGGGAGGGAGCGACAAGGGGGTGAAGATCGGGCA
GCTGGCCGACATGTACGCCGACGTCACCAAAGGTTGGGCGGATTCGTGA

Upstream 100 bases:

>100_bases
GCCACTACGGTCCCGTGGCCGTTGGGGCTCCCGACGGGCGGATCGCCGACGAGGTCGGTGTACTGGTGGACAAATTCGCG
GCGCTCGTGAAAAGGATCTG

Downstream 100 bases:

>100_bases
GGGGGTAGTTCATGAACGGGAAAACAGGGTTCATCGGCATCATCCTTTTGGCGCTGGTTCCGTGGAATGCTGGCGCCACC
GAGACCGGCACCATGATCTG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTAGGLHNGSGGMESARHCRELVERWLADGSFDARLPEVVRLRGVLQPEQYHAEGDAWTHTMLAMEAVPDDADPRVFWGV
LLHDIGKAETTAFIKGRWRSWGHAEAGAGEVSAIMTRLGFPHLAADVTWLVKHHTFHFSWNLTPGARLSPKQRRFMEHPL
FPLLLEVCDADAAGSRGGSDKGVKIGQLADMYADVTKGWADS

Sequences:

>Translated_202_residues
MTAGGLHNGSGGMESARHCRELVERWLADGSFDARLPEVVRLRGVLQPEQYHAEGDAWTHTMLAMEAVPDDADPRVFWGV
LLHDIGKAETTAFIKGRWRSWGHAEAGAGEVSAIMTRLGFPHLAADVTWLVKHHTFHFSWNLTPGARLSPKQRRFMEHPL
FPLLLEVCDADAAGSRGGSDKGVKIGQLADMYADVTKGWADS
>Mature_201_residues
TAGGLHNGSGGMESARHCRELVERWLADGSFDARLPEVVRLRGVLQPEQYHAEGDAWTHTMLAMEAVPDDADPRVFWGVL
LHDIGKAETTAFIKGRWRSWGHAEAGAGEVSAIMTRLGFPHLAADVTWLVKHHTFHFSWNLTPGARLSPKQRRFMEHPLF
PLLLEVCDADAAGSRGGSDKGVKIGQLADMYADVTKGWADS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 22238; Mature: 22107

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGGLHNGSGGMESARHCRELVERWLADGSFDARLPEVVRLRGVLQPEQYHAEGDAWTH
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCCHHHH
TMLAMEAVPDDADPRVFWGVLLHDIGKAETTAFIKGRWRSWGHAEAGAGEVSAIMTRLGF
HHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PHLAADVTWLVKHHTFHFSWNLTPGARLSPKQRRFMEHPLFPLLLEVCDADAAGSRGGSD
CHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
KGVKIGQLADMYADVTKGWADS
CCEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAGGLHNGSGGMESARHCRELVERWLADGSFDARLPEVVRLRGVLQPEQYHAEGDAWTH
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCCCCHHHH
TMLAMEAVPDDADPRVFWGVLLHDIGKAETTAFIKGRWRSWGHAEAGAGEVSAIMTRLGF
HHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PHLAADVTWLVKHHTFHFSWNLTPGARLSPKQRRFMEHPLFPLLLEVCDADAAGSRGGSD
CHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
KGVKIGQLADMYADVTKGWADS
CCEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA