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The map label for this gene is hisF

Identifier: 78188420

GI number: 78188420

Start: 517332

End: 518087

Strand: Reverse

Name: hisF

Synonym: Cag_0442

Alternate gene names: 78188420

Gene position: 518087-517332 (Counterclockwise)

Preceding gene: 78188425

Following gene: 78188419

Centisome position: 20.14

GC content: 48.15

Gene sequence:

>756_bases
ATGTTGGCAAAAAGAATTATCCCCTGCCTTGATGTGCGGGATGGACGAGTGGTTAAAGGCATTAATTTTGAAGGGTTACG
CGATGCAGGCTCTATTTTAGAGCAAGCACGTTTTTACAATAACGAACTGGCTGACGAGCTGGTTTTTCTCGACATTTCTG
CCTCGCTTGAGTCGCGCCGCACCACGCTTGAAGAGGTAATGAAAGTGTCGGAAGAGGTCTTTATTCCGCTGACGGTTGGC
GGTGGCATCAGCTCGGTTGAACGGGCACGCGAAGTTTTTTTACATGGTGCCGATAAGGTATCGGTAAACACGGCTGCCGT
TAACGATCCCTATTTGATTAGCCGCATTGCCGAAAAGTATGGTTCGCAAGCAGTAGTGGTGGCAATTGATATTAAAAAAG
TGGGGAACCACTACATGGTGCATACCCACTCAGGCAAGCAAATCACGCAATACGAGGCGCTTGAATGGGCGCTGAAAGTG
CAAGAGCTTGGAGCGGGCGAAATTCTTTTGACCAGCATGGATCGCGATGGCACCAAAGAAGGGTACGAAAACAACAGTTT
GCGCATGATTTCAACAGCCGTTCATATTCCCGTGATTGCTTCAGGCGGAGCGGGCAATTTGGAACACTTGTACGATGGGT
TTAGTAAAGGCTGTGCAGATGCTGCGCTTGCTGCCTCAATTTTCCATTTTCGCCATTACTCCATTCGGCAGGCAAAAGAG
TATTTGCACAAACGTGGAGTTGCTGTAAGGTTTTAA

Upstream 100 bases:

>100_bases
GAGCGAAAGCTCTGGCTGTTTGAATTTTTGTTCCTGAATTATGGAGAGTAGTTGTTACCGTATAGCAGTACTCCCCACCA
TTTTTTAAGTAGTAAAAAAT

Downstream 100 bases:

>100_bases
TGAAGATTTAAGTTTGGCTTTACTCTTGTTTAAAGAAAAGAGAACTACCTTAACTTTCGTTGAACTGCCAATCTTTAAAC
AAAACAAATTACTATGAGTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRRTTLEEVMKVSEEVFIPLTVG
GGISSVERAREVFLHGADKVSVNTAAVNDPYLISRIAEKYGSQAVVVAIDIKKVGNHYMVHTHSGKQITQYEALEWALKV
QELGAGEILLTSMDRDGTKEGYENNSLRMISTAVHIPVIASGGAGNLEHLYDGFSKGCADAALAASIFHFRHYSIRQAKE
YLHKRGVAVRF

Sequences:

>Translated_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRRTTLEEVMKVSEEVFIPLTVG
GGISSVERAREVFLHGADKVSVNTAAVNDPYLISRIAEKYGSQAVVVAIDIKKVGNHYMVHTHSGKQITQYEALEWALKV
QELGAGEILLTSMDRDGTKEGYENNSLRMISTAVHIPVIASGGAGNLEHLYDGFSKGCADAALAASIFHFRHYSIRQAKE
YLHKRGVAVRF
>Mature_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRRTTLEEVMKVSEEVFIPLTVG
GGISSVERAREVFLHGADKVSVNTAAVNDPYLISRIAEKYGSQAVVVAIDIKKVGNHYMVHTHSGKQITQYEALEWALKV
QELGAGEILLTSMDRDGTKEGYENNSLRMISTAVHIPVIASGGAGNLEHLYDGFSKGCADAALAASIFHFRHYSIRQAKE
YLHKRGVAVRF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.136186770428, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082028, Length=216, Percent_Identity=27.3148148148148, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=34.5047923322684, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CHLCH (Q3ATG0)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378758.1
- ProteinModelPortal:   Q3ATG0
- SMR:   Q3ATG0
- STRING:   Q3ATG0
- GeneID:   3748148
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0442
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   CCHL340177:CAG_0442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27666; Mature: 27666

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVMKVSEEVFIPLTVGGGISSVERAREVFLHGADKVSVNTAAVNDPYLISRIAEKY
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHH
GSQAVVVAIDIKKVGNHYMVHTHSGKQITQYEALEWALKVQELGAGEILLTSMDRDGTKE
CCCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYENNSLRMISTAVHIPVIASGGAGNLEHLYDGFSKGCADAALAASIFHFRHYSIRQAKE
CCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLHKRGVAVRF
HHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNELADELVFLDISASLESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
TTLEEVMKVSEEVFIPLTVGGGISSVERAREVFLHGADKVSVNTAAVNDPYLISRIAEKY
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHH
GSQAVVVAIDIKKVGNHYMVHTHSGKQITQYEALEWALKVQELGAGEILLTSMDRDGTKE
CCCEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYENNSLRMISTAVHIPVIASGGAGNLEHLYDGFSKGCADAALAASIFHFRHYSIRQAKE
CCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLHKRGVAVRF
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA