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The map label for this gene is rpoD [H]

Identifier: 78188365

GI number: 78188365

Start: 448296

End: 449195

Strand: Reverse

Name: rpoD [H]

Synonym: Cag_0387

Alternate gene names: 78188365

Gene position: 449195-448296 (Counterclockwise)

Preceding gene: 78188366

Following gene: 78188364

Centisome position: 17.46

GC content: 47.11

Gene sequence:

>900_bases
ATGCGGCAACTAAAAATCAGTAAACAAATAACCAATCGCGAAAGCCTCTCTCTCGACCGCTATTTGCAAGAGATTGGTAA
ATATGATCTGCTCACTGCCGAAGATGAGGTGCGCCTTACCAAAGCCATTAAAGAGGGCTTTGATATGCCGGTTGACACCC
CCGAATACAAGCGGGCAAAACGCGCACTTGATAAGCTTATTAAAGGCAATCTTCGCTTTGTGGTTTCGGTAGCTAAGCAA
TACCAAAACCAAGGGTTAACGCTTGGCGACCTTATTAATGAAGGGAATCTTGGACTTATTAAAGCCGCAAAACGCTTTGA
TGAAACACGCGGCTTTAAATTTATTTCCTACGCTGTGTGGTGGATTCGCCAATCCATTTTGCAAGCTCTTGCTGAGCAAT
CGCGCATTGTGCGCTTGCCACTGAACCGCGTTGGTACGCTGAACAAAATCAGCAAAGCATATAGCCAACTTGAGCAAGAG
TACGAGCGCGATCCCAACACACGCGAACTTGCAAGCTTGCTTGAAATGGATTCGCAAGATGTTGCCGACACGCTTAAAAT
TGCCGGACGCCATGTGTCGGTTGATGCACCCTTTGCGCAAGGCGACGACAACCGCTTGCTTGACGTGTTGCAAAATGATG
GACATATGCCCGATTACGGCTTAACCCGCGATTCTCTAACGCTTGAAGTTGAACGTTCACTTTCGGTGCTCGCTCCCCGC
GAAGCCGATGTTATTCGCTCTTACTTTGGCATTGGTATGGATAATCCGCTAACGTTGGAGGAGATTGGCGAAAAATTCCG
TTTAACCCGTGAACGTGTTCGCCAAATTAAAGAAAAGGCTATTCGGCGGTTACGCCAATCGGCATATAGCAAAGTGCTCA
AAGAGTATATTGGCGGATAA

Upstream 100 bases:

>100_bases
AAACGTACATTCCCGCCCCTCTGGGACTTTTTAAAGGAACCAGATTGTTTGGGTAATAAGTTTAGCCCACAATCACCGAG
CTTGAAAGGAGTTATCTGCT

Downstream 100 bases:

>100_bases
CAACCGTTGCATAAAAAGCACATTTGAAAGGAGCAATTGGCAACCAAAAGGCGCTGATTGCTCCTTTGCTGTTTTGGGAA
CATTGTACATCTCCCCCCTC

Product: RNA polymerase sigma factor rpoD (sigma-A)

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYDLLTAEDEVRLTKAIKEGFDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
YERDPNTRELASLLEMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQNDGHMPDYGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMDNPLTLEEIGEKFRLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGG

Sequences:

>Translated_299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYDLLTAEDEVRLTKAIKEGFDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
YERDPNTRELASLLEMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQNDGHMPDYGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMDNPLTLEEIGEKFRLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGG
>Mature_299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYDLLTAEDEVRLTKAIKEGFDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
YERDPNTRELASLLEMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQNDGHMPDYGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMDNPLTLEEIGEKFRLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=41.0788381742739, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=38.2022471910112, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=27.1777003484321, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34293; Mature: 34293

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLKISKQITNRESLSLDRYLQEIGKYDLLTAEDEVRLTKAIKEGFDMPVDTPEYKRAK
CCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
RALDKLIKGNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVW
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
WIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQEYERDPNTRELASLLEMDSQD
HHHHHHHHHHHHCCCEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
VADTLKIAGRHVSVDAPFAQGDDNRLLDVLQNDGHMPDYGLTRDSLTLEVERSLSVLAPR
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCC
EADVIRSYFGIGMDNPLTLEEIGEKFRLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQLKISKQITNRESLSLDRYLQEIGKYDLLTAEDEVRLTKAIKEGFDMPVDTPEYKRAK
CCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
RALDKLIKGNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVW
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
WIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQEYERDPNTRELASLLEMDSQD
HHHHHHHHHHHHCCCEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
VADTLKIAGRHVSVDAPFAQGDDNRLLDVLQNDGHMPDYGLTRDSLTLEVERSLSVLAPR
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCC
EADVIRSYFGIGMDNPLTLEEIGEKFRLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]