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The map label for this gene is gltD

Identifier: 78188168

GI number: 78188168

Start: 207912

End: 209387

Strand: Reverse

Name: gltD

Synonym: Cag_0185

Alternate gene names: 78188168

Gene position: 209387-207912 (Counterclockwise)

Preceding gene: 78188169

Following gene: 78188167

Centisome position: 8.14

GC content: 48.51

Gene sequence:

>1476_bases
ATGGGAAAAATTAAAGGCTTTATGGAATACAAAAGAGCGTTGCCTGCTGATAGGCAACCTCTTGAACGAATTAAGGATTG
GCAGGAGTTCCACGAAAAAATGGCACCTGAAGCTTTATGCGAACAAGGTGCGCGTTGCATGGATTGCGGAACGCCATATT
GCCATAGCGGCATTATGCTGAGCGGTATGACCACAGGCTGCCCTATTCACAACTTAATTCCTGAATGGAACGACTACGTT
TACCGCGGTTTTTGGCACGAAGCCTACGATAGGCTCAATAAAACCAACAATTTCCCAGAGTTTACGGGACGCGTTTGCCC
TGCCCCTTGCGAAGGCTCTTGCGTGCTTGGCATTATTAATCCACCGGTTACCATTAAAAACATTGAGTACTCCATTATTG
AGCACGCCTTTGCTGAAGGATGGGTTACCCCTAAAACCATTGCAAACCGCACAGGCAAACGAGTTGCCGTTGTGGGTTCT
GGTCCATCAGGCTTAGCATGTGCCGATCAGCTTAACAAAGCGGGGCATAGCGTTACGGTTTTTGAGCGTGATGACCGTTG
CGGTGGGCTTTTAATGTATGGTATTCCCAACATGAAGCTAGATAAAGTGCAAGTGGTTGAGCGCCGCATTGCTTTAATGA
AGCAGGAAGGCATCACCTTTATGGAAAAGACGGAGGTGGGTGTTGATTATCCCGCAGGTAAATTGCTCGAAGAGTTTGAT
GCCGTTGTGCTTTGCACGGGTGCTACCAAACCACGCGATCTTGCTGAAGAGGGACGCCAGCTTGCAGGCATCCATTTTGC
AATGGAATTCTTAACCGCCAGCACAAAAGCCCTGCTTGATGGCACCGAGCCAAAACTTTCAGCAAAAGGCAAAAAAGTGG
TAGTAATTGGCGGTGGCGATACGGGTACCGACTGTGTAGCAACCTCACTTCGCCAAAAATGCGCAAGTGTGGTACAGCTT
GAAATTATGCCAAAACCCCCCATGGAACGCCAAGCCGACAATCCATGGCCTGAATGGCCAAAAGTGTTCCGTGTAGATTA
CGGGCAGGAAGAGGCTGAAGCGCTGCAAGGGAGCGATCCTCGCCGTTACGCCATGATGACAAAAAAATTCCTTACAACAG
GAAATGGCAACGTTTCTGGCGTTGAAGTGTGTAGCATAGCGTGGGAAAACATTGATGGACGCTTTGTACCAACCCCAATT
CCCGGTACTGAAGAGATTATTGAAGCCGATATGGTATTGCTTGCTTTAGGCTTTATTGGAGCTGAAGAGAACCTTTTACA
ACAACTTCAGGTTGCACAAGATGAACGTTCCAACATTAAAGCCAATACCCAAAACTACCGCACAAACCATGAACGCATTT
TTGCCGCAGGCGATGCGCGCCGCGGTCAAAGCTTAGTGGTGTGGGCAATTCAAGAAGGACGAGCTGCCGCTCGCGAATGC
GATCGCTTTTTAATGGGCGGCACCAATTTGCCATAA

Upstream 100 bases:

>100_bases
CTGGTTTAAGTGGCGACGAAGCGGTAATGGCTGCATTTGAAAAGAATATTCACGATCCAGCAAGGGTGTCAGGGAATTAA
TGATTGACTAACGCATAGTT

Downstream 100 bases:

>100_bases
TTCTGCTGAAAACAATTTCTATGGAACAGCAAAAACTCCGTGAGCTGCTACAAGCCTTGCATCAAGAGCTTGAGCAGCTC
CAAAGCGTTGATGAATCCAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MGKIKGFMEYKRALPADRQPLERIKDWQEFHEKMAPEALCEQGARCMDCGTPYCHSGIMLSGMTTGCPIHNLIPEWNDYV
YRGFWHEAYDRLNKTNNFPEFTGRVCPAPCEGSCVLGIINPPVTIKNIEYSIIEHAFAEGWVTPKTIANRTGKRVAVVGS
GPSGLACADQLNKAGHSVTVFERDDRCGGLLMYGIPNMKLDKVQVVERRIALMKQEGITFMEKTEVGVDYPAGKLLEEFD
AVVLCTGATKPRDLAEEGRQLAGIHFAMEFLTASTKALLDGTEPKLSAKGKKVVVIGGGDTGTDCVATSLRQKCASVVQL
EIMPKPPMERQADNPWPEWPKVFRVDYGQEEAEALQGSDPRRYAMMTKKFLTTGNGNVSGVEVCSIAWENIDGRFVPTPI
PGTEEIIEADMVLLALGFIGAEENLLQQLQVAQDERSNIKANTQNYRTNHERIFAAGDARRGQSLVVWAIQEGRAAAREC
DRFLMGGTNLP

Sequences:

>Translated_491_residues
MGKIKGFMEYKRALPADRQPLERIKDWQEFHEKMAPEALCEQGARCMDCGTPYCHSGIMLSGMTTGCPIHNLIPEWNDYV
YRGFWHEAYDRLNKTNNFPEFTGRVCPAPCEGSCVLGIINPPVTIKNIEYSIIEHAFAEGWVTPKTIANRTGKRVAVVGS
GPSGLACADQLNKAGHSVTVFERDDRCGGLLMYGIPNMKLDKVQVVERRIALMKQEGITFMEKTEVGVDYPAGKLLEEFD
AVVLCTGATKPRDLAEEGRQLAGIHFAMEFLTASTKALLDGTEPKLSAKGKKVVVIGGGDTGTDCVATSLRQKCASVVQL
EIMPKPPMERQADNPWPEWPKVFRVDYGQEEAEALQGSDPRRYAMMTKKFLTTGNGNVSGVEVCSIAWENIDGRFVPTPI
PGTEEIIEADMVLLALGFIGAEENLLQQLQVAQDERSNIKANTQNYRTNHERIFAAGDARRGQSLVVWAIQEGRAAAREC
DRFLMGGTNLP
>Mature_490_residues
GKIKGFMEYKRALPADRQPLERIKDWQEFHEKMAPEALCEQGARCMDCGTPYCHSGIMLSGMTTGCPIHNLIPEWNDYVY
RGFWHEAYDRLNKTNNFPEFTGRVCPAPCEGSCVLGIINPPVTIKNIEYSIIEHAFAEGWVTPKTIANRTGKRVAVVGSG
PSGLACADQLNKAGHSVTVFERDDRCGGLLMYGIPNMKLDKVQVVERRIALMKQEGITFMEKTEVGVDYPAGKLLEEFDA
VVLCTGATKPRDLAEEGRQLAGIHFAMEFLTASTKALLDGTEPKLSAKGKKVVVIGGGDTGTDCVATSLRQKCASVVQLE
IMPKPPMERQADNPWPEWPKVFRVDYGQEEAEALQGSDPRRYAMMTKKFLTTGNGNVSGVEVCSIAWENIDGRFVPTPIP
GTEEIIEADMVLLALGFIGAEENLLQQLQVAQDERSNIKANTQNYRTNHERIFAAGDARRGQSLVVWAIQEGRAAARECD
RFLMGGTNLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=479, Percent_Identity=21.294363256785, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=36.6803278688525, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788811, Length=487, Percent_Identity=33.6755646817248, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082180, Length=469, Percent_Identity=33.0490405117271, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=24.1150442477876, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=26.1176470588235, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=50.6024096385542, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71984108, Length=479, Percent_Identity=23.1732776617954, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=50, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI28574881, Length=488, Percent_Identity=54.9180327868852, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665539, Length=488, Percent_Identity=54.9180327868852, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665547, Length=488, Percent_Identity=54.9180327868852, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24665543, Length=488, Percent_Identity=54.9180327868852, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24640763, Length=302, Percent_Identity=26.8211920529801, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI18858217, Length=302, Percent_Identity=26.8211920529801, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54239; Mature: 54108

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIKGFMEYKRALPADRQPLERIKDWQEFHEKMAPEALCEQGARCMDCGTPYCHSGIML
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCCCCHHCCCEEE
SGMTTGCPIHNLIPEWNDYVYRGFWHEAYDRLNKTNNFPEFTGRVCPAPCEGSCVLGIIN
ECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIEYSIIEHAFAEGWVTPKTIANRTGKRVAVVGSGPSGLACADQLNKAGHSVTV
CCEEEECCHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FERDDRCGGLLMYGIPNMKLDKVQVVERRIALMKQEGITFMEKTEVGVDYPAGKLLEEFD
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCC
AVVLCTGATKPRDLAEEGRQLAGIHFAMEFLTASTKALLDGTEPKLSAKGKKVVVIGGGD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
TGTDCVATSLRQKCASVVQLEIMPKPPMERQADNPWPEWPKVFRVDYGQEEAEALQGSDP
CCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCC
RRYAMMTKKFLTTGNGNVSGVEVCSIAWENIDGRFVPTPIPGTEEIIEADMVLLALGFIG
HHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCC
AEENLLQQLQVAQDERSNIKANTQNYRTNHERIFAAGDARRGQSLVVWAIQEGRAAAREC
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHH
DRFLMGGTNLP
HHHHCCCCCCC
>Mature Secondary Structure 
GKIKGFMEYKRALPADRQPLERIKDWQEFHEKMAPEALCEQGARCMDCGTPYCHSGIML
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCCCCHHCCCEEE
SGMTTGCPIHNLIPEWNDYVYRGFWHEAYDRLNKTNNFPEFTGRVCPAPCEGSCVLGIIN
ECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEEEEC
PPVTIKNIEYSIIEHAFAEGWVTPKTIANRTGKRVAVVGSGPSGLACADQLNKAGHSVTV
CCEEEECCHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
FERDDRCGGLLMYGIPNMKLDKVQVVERRIALMKQEGITFMEKTEVGVDYPAGKLLEEFD
EECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCC
AVVLCTGATKPRDLAEEGRQLAGIHFAMEFLTASTKALLDGTEPKLSAKGKKVVVIGGGD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
TGTDCVATSLRQKCASVVQLEIMPKPPMERQADNPWPEWPKVFRVDYGQEEAEALQGSDP
CCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCCC
RRYAMMTKKFLTTGNGNVSGVEVCSIAWENIDGRFVPTPIPGTEEIIEADMVLLALGFIG
HHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHCC
AEENLLQQLQVAQDERSNIKANTQNYRTNHERIFAAGDARRGQSLVVWAIQEGRAAAREC
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHH
DRFLMGGTNLP
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]