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The map label for this gene is nagE [H]

Identifier: 78067620

GI number: 78067620

Start: 3296122

End: 3297891

Strand: Reverse

Name: nagE [H]

Synonym: Bcep18194_A6151

Alternate gene names: 78067620

Gene position: 3297891-3296122 (Counterclockwise)

Preceding gene: 78067621

Following gene: 78067613

Centisome position: 89.27

GC content: 67.8

Gene sequence:

>1770_bases
ATGAACGGGAATCCGTTTCTGAAAATACAGGGCCTGGGCCGGGCACTGATGCTACCGATCGCGGTACTGCCGGTTGCCGG
CATCCTGCTGCGACTCGGCCAGCCGGACGTGTTCAACATCAAGATGATCGCCGACGCCGGCGGCGCGATTTTCGACAACC
TGCCGCTGCTGTTCGCGATCGGCGTGGCGGTCGGTTTCGCGAAGGACAACAACGGCGTCGCGGCGCTCGCGGGCGCGATC
GGTTACCTGATCGAAACCGCGATCATGAAGGACATCGATCCCAAGCTGAACATGGGCGTGCTGTCCGGGATCATCGCGGG
TGTGGTCGCGGGCCTGCTGTACAACCGCTATAAAGACATCAAGCTGCCGGACTACCTCGCGTTCTTCGGCGGCAAACGGT
TCGTACCGATCATCACGGGGCTGGTCTGCGTGATACTCGGCATCGCGTTCGGATACGTCTGGCAGCCGGTCCAGCATCTG
ATCGACGCGGCCGGCCAGTGGCTGACGACGGCGGGCGCGATCGGTGCGTTCGTGTTCGGCTTCCTGAACCGCCTGCTGCT
CGTCACGGGCCTGCACCACATCCTCAACTCGCTCGCATGGTTCGTGTTCGGCAACTTCACGCCGCCCGCCGGCGGCGAGA
TCGTGCACGGCGACCTGCACCGCTTCTTCGCGGGCGACCCGACCGCGGGCACCTTCATGGCCGGCTTCTTCCCGATCATG
ATGTTCGGCCTGCCGGCGGCGTGTCTGGCGATGCTGCACGAAGCGCCGAAGGAACGCCGCGCGATGGTCGGCGGCCTGCT
GCTGTCGATGGCGCTGACGTCGTTCCTGACCGGCGTGACCGAGCCGATCGAATTCAGCTTCATGTTCCTCGCACCGGTGC
TGTACGTGATCCACGCGGTGCTGACGGGGCTGTCGCTCGCGATCTGCCAGATCCTCGGCGTGAAGCTCGGCTTCACGTTC
TCGGCCGGTGCGATCGACTACGTGCTGAACTACGGGCTGTCGACGAAGGGCTGGATCGCGATTCCGCTTGGCATCGCGTA
CGGCGCCGCGTACTACGGGCTGTTCCGCTTCTTCATCCGCAAGTTCAACATGGCCACGCCGGGCCGTGAGCCGGCATCGG
CCGATGCGGCTGCTGAATCGTACGCATCGGGCGGCTTCGTTGCGCCGGCTGCCGGCGCCGCTGCGGTTGCCGCAGCACCG
CGCGCGCAGCGCTATATCGCCGCGCTCGGCGGGGCAGGCAACCTGTCGGTCGTCGACGCGTGCACGACGCGCCTGCGCCT
GACCGTCGTCGATCCGGAGAAGGTTTCGGAGCCGGAACTGAAATCGATCGGCGCGCGCGGCGTGCTCAAGCGCGGCGGCA
ACAGCGTGCAGGTGATCATCGGGCCCGAGGCCGACATCATTGCCGACGAGATGCGTGCCGCCATCGGCAGCGGTGCGGCA
GGCGCAGCATCGACGGCGACGGCCGCCGCCGCGCAGCCCGTCACGGGCGCGGCCGCCGGCCCGCTCGATCCGGAGCCGAC
GCGCTGGCTCGCGGTGTTCGGCGGCGCGACCAACGTCGCGTCGCTCGATGCGGTTGCGACCACGCGCCTGCGTGTGGTCG
TACGCGATCCGTCGGCGGTCGATCGCGATCGTCTCGGTGCGCTCGACGTCGCGTGGGTGTCGCTCGACACGTTCCACATC
GTCTGCGGCAACGCGGCGGCACGCTATGCCGAGCAGCTCGGCGCGCGCCTGCCGCCGTCGGGCAGCGGGGCGGCCGTACA
ACCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCTCGACTGAGCGTCGACGGCAGGTTCGCGACCGGTTTCAAGACGAAAGCAATTAGGTCAACGACGATTAATGACGA
GACAAGGATTGGAGGACTGA

Downstream 100 bases:

>100_bases
TGCGATGAAGCCAGCCTGATCCGCAATGCGGGTTCGGGCCGGCCGGCAAAACGAAAACGGCTTGCGATGCGTCGCAAGCC
GTTTTTCTTTGGTGCGGGCA

Product: PTS system, N-acetylglucosamine-specific IIBC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MNGNPFLKIQGLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVILGIAFGYVWQPVQHL
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHILNSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLLSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGAAYYGLFRFFIRKFNMATPGREPASADAAAESYASGGFVAPAAGAAAVAAAP
RAQRYIAALGGAGNLSVVDACTTRLRLTVVDPEKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIGSGAA
GAASTATAAAAQPVTGAAAGPLDPEPTRWLAVFGGATNVASLDAVATTRLRVVVRDPSAVDRDRLGALDVAWVSLDTFHI
VCGNAAARYAEQLGARLPPSGSGAAVQPA

Sequences:

>Translated_589_residues
MNGNPFLKIQGLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVILGIAFGYVWQPVQHL
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHILNSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLLSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGAAYYGLFRFFIRKFNMATPGREPASADAAAESYASGGFVAPAAGAAAVAAAP
RAQRYIAALGGAGNLSVVDACTTRLRLTVVDPEKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIGSGAA
GAASTATAAAAQPVTGAAAGPLDPEPTRWLAVFGGATNVASLDAVATTRLRVVVRDPSAVDRDRLGALDVAWVSLDTFHI
VCGNAAARYAEQLGARLPPSGSGAAVQPA
>Mature_589_residues
MNGNPFLKIQGLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVILGIAFGYVWQPVQHL
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHILNSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLLSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGAAYYGLFRFFIRKFNMATPGREPASADAAAESYASGGFVAPAAGAAAVAAAP
RAQRYIAALGGAGNLSVVDACTTRLRLTVVDPEKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIGSGAA
GAASTATAAAAQPVTGAAAGPLDPEPTRWLAVFGGATNVASLDAVATTRLRVVVRDPSAVDRDRLGALDVAWVSLDTFHI
VCGNAAARYAEQLGARLPPSGSGAAVQPA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=472, Percent_Identity=56.1440677966102, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1787343, Length=496, Percent_Identity=43.9516129032258, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787908, Length=523, Percent_Identity=36.9024856596558, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61084; Mature: 61084

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNPFLKIQGLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KLPDYLAFFGGKRFVPIITGLVCVILGIAFGYVWQPVQHLIDAAGQWLTTAGAIGAFVFG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLNRLLLVTGLHHILNSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHH
MFGLPAACLAMLHEAPKERRAMVGGLLLSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQILGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGIAYGAAYYGLFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
KFNMATPGREPASADAAAESYASGGFVAPAAGAAAVAAAPRAQRYIAALGGAGNLSVVDA
HHCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHCHHHHHHHHHHCCCCCCHHHHH
CTTRLRLTVVDPEKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIGSGAA
HCCEEEEEEECHHHCCCCHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
GAASTATAAAAQPVTGAAAGPLDPEPTRWLAVFGGATNVASLDAVATTRLRVVVRDPSAV
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHEEEEEEEEECCCCC
DRDRLGALDVAWVSLDTFHIVCGNAAARYAEQLGARLPPSGSGAAVQPA
CHHHCCCEEEEEEEECEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGNPFLKIQGLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KLPDYLAFFGGKRFVPIITGLVCVILGIAFGYVWQPVQHLIDAAGQWLTTAGAIGAFVFG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLNRLLLVTGLHHILNSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHH
MFGLPAACLAMLHEAPKERRAMVGGLLLSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQILGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGIAYGAAYYGLFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
KFNMATPGREPASADAAAESYASGGFVAPAAGAAAVAAAPRAQRYIAALGGAGNLSVVDA
HHCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHCHHHHHHHHHHCCCCCCHHHHH
CTTRLRLTVVDPEKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIGSGAA
HCCEEEEEEECHHHCCCCHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
GAASTATAAAAQPVTGAAAGPLDPEPTRWLAVFGGATNVASLDAVATTRLRVVVRDPSAV
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHEEEEEEEEECCCCC
DRDRLGALDVAWVSLDTFHIVCGNAAARYAEQLGARLPPSGSGAAVQPA
CHHHCCCEEEEEEEECEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]