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The map label for this gene is rpoN [H]

Identifier: 78067590

GI number: 78067590

Start: 3265012

End: 3266517

Strand: Direct

Name: rpoN [H]

Synonym: Bcep18194_A6121

Alternate gene names: 78067590

Gene position: 3265012-3266517 (Clockwise)

Preceding gene: 78067589

Following gene: 78067591

Centisome position: 88.38

GC content: 64.08

Gene sequence:

>1506_bases
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAACCCGCTGCG
ACAAACAGCAGCAGTTCGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGCTC
CGGCGACGGCAACGATGGCCCGCAGTGGAACCTCGACGAATTCGGGCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAAGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACGGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGATTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGTGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAA
CGACGATGCGCTGCGCGACGCGCACACGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATGGTGAAGAAGGTCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGTGATCCGTCGGCCGGTTCGCTCAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCTTCGTCGACGGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Upstream 100 bases:

>100_bases
TCGCGGCCATGCCCGCGACCGGGCGGCGCGCCGCGCGTAATCGCGGATGGCTCAACGCGCCTGGGTCGTGGCAAACTGCC
GGTATGACTCCCTCCTTGCC

Downstream 100 bases:

>100_bases
GCCTGCTGCCTGCCCGGCGGCAGGCGTGTTCCGGCCACCGCGCATACGGGGAACAGGCCGGGCGACCTGTCCGCGATACA
CCGCCTCGTGCGGGTCGGGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Sequences:

>Translated_501_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL
>Mature_501_residues
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=503, Percent_Identity=44.7316103379722, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54890; Mature: 54890

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRV
CCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHE
AADGSLIAQTPPAQNTEPAATNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDE
ECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASPRDRALVMFLIESLDDDGYLT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCEEE
ATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCCCCHHHH
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
IMVKKVGQGWLAELNPEVVPRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQ
HHHHHHCCCHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSRVTTGKYMLTPFG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCC
TLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHH
RTVAKYREALKIPAVNLRKSL
HHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRV
CCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHE
AADGSLIAQTPPAQNTEPAATNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDE
ECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASPRDRALVMFLIESLDDDGYLT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCEEE
ATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCCCCHHHH
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
IMVKKVGQGWLAELNPEVVPRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQ
HHHHHHCCCHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSRVTTGKYMLTPFG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCC
TLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHH
RTVAKYREALKIPAVNLRKSL
HHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]