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The map label for this gene is purD [H]

Identifier: 78067091

GI number: 78067091

Start: 2721602

End: 2722879

Strand: Direct

Name: purD [H]

Synonym: Bcep18194_A5622

Alternate gene names: 78067091

Gene position: 2721602-2722879 (Clockwise)

Preceding gene: 78067090

Following gene: 78067092

Centisome position: 73.67

GC content: 67.29

Gene sequence:

>1278_bases
ATGAAACTACTCGTCGTCGGTTCCGGCGGCCGCGAACATGCGCTGGCGTGGAAGCTCGCGCAGTCGCCGCGCGTCCAGAT
GGTCTACGTCGCGCCCGGCAATGGCGGCACGGCGCAGGACGAGCGCCTGAAGAACGTCGACATCACGGCGCTCGACGAAC
TCGCCGATTTCGCGGAACGCGAAGGCGTCGCGTTCACGCTCGTCGGGCCGGAAGCACCGCTCGCGGCCGGCATCGTCAAC
CTGTTCCGCGCACGCGGCCTGAAGGTCTTCGGCCCGACCCGCGAAGCCGCGCAGCTCGAGAGCTCGAAGGATTTCGCGAA
GGCGTTCATGAAGCGCCACGGCATCCCGACCGCCGAGTACGAAACGTTCTCCGACGTGGCCGCCGCGCACGCGTACATCG
ACGCGAAGGGTGCGCCGATCGTCGTGAAGGCGGACGGCCTCGCGGCCGGCAAGGGTGTCGTCGTCGCGATGACGCTGGAA
GAAGCACACGAAGCCGTCGACATGATGCTGTCGGGCAACAAGCTCGGCGATGCCGGCGCGCGCGTCGTGATCGAGGAATT
CCTCGATGGCGAGGAAGCGAGCTTCATCGTGATGGTCGACGGCAAGCACGCGCTGGCGCTGGCTTCCAGCCAGGACCACA
AGCGCCTGCTCGACGAGGACCGCGGCCCGAACACCGGCGGCATGGGCGCGTATTCGCCCGCGCCGATCGTCACGCCGCAG
ATGCATGCACGCGTGATGCGCGAGATCATCATGCCGACCGTGCGCGGGATGGAGAAGGACGGCATCCGCTTCACGGGCTT
CCTGTACGCGGGCCTGATGATCGACAAGGAAGGCAATCCGCGCACGCTCGAGTTCAACTGCCGGATGGGCGACCCGGAAA
CGCAGCCGATCATGGCGCGCCTGAAGAGCGATTTCTCGAAGGTCGTCGAGCAGGCGATCGCGGGCACGCTCGACACGGTC
GAGCTCGACTGGGACCGCCGCACCGCGCTGGGCGTCGTGCTGGCCGCGCACGGTTATCCGGATGCGCCGCGCAAGGGCGA
CCGCATCAACGGGATCCCGGCCGAGACCGAGCAGACGGTCACGTTCCATGCGGGCACGACGCTCGACGGCGACAAGCTGA
CGACGTCCGGCGGCCGCGTGCTGTGTGTGGTCGGCCTCGCCGATTCGGTCCGCGAAGCGCAGCAGCATGCGTACGAGACG
ATCAACCAGATCAATTTCGAAGGCATGCAGTACCGCCGCGACATCGGCTTCCGCGCGCTCAACCGCAAGAGCGCGTGA

Upstream 100 bases:

>100_bases
CCGGCCGCCGTGCTCCTGTCGCGGCGGCGGCCCGACCGAATCCGCGGCCGGCGCGCCTGAGCGTGCCGGCCGCCCTGTTT
TCTAGTCTGCGGGGAATCCC

Downstream 100 bases:

>100_bases
CGTTGGAATTGCCGCCCCGCCTTCGTGCGGCGGCGGCCCCTCTGCCGGGGTTCGTTAGAATGCCCGGCAGAAGCCTTTTT
TACGGCCCCCGCTTCGGGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHGIPTAEYETFSDVAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQTVTFHAGTTLDGDKLTTSGGRVLCVVGLADSVREAQQHAYET
INQINFEGMQYRRDIGFRALNRKSA

Sequences:

>Translated_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHGIPTAEYETFSDVAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQTVTFHAGTTLDGDKLTTSGGRVLCVVGLADSVREAQQHAYET
INQINFEGMQYRRDIGFRALNRKSA
>Mature_425_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHGIPTAEYETFSDVAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHEAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQTVTFHAGTTLDGDKLTTSGGRVLCVVGLADSVREAQQHAYET
INQINFEGMQYRRDIGFRALNRKSA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=60.7476635514019, Blast_Score=520, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=44.6808510638298, Blast_Score=350, Evalue=6e-97,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=42.3162583518931, Blast_Score=347, Evalue=2e-96,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.707656612529, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=45.7471264367816, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45822; Mature: 45822

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHH
EGVAFTLVGPEAPLAAGIVNLFRARGLKVFGPTREAAQLESSKDFAKAFMKRHGIPTAEY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDVAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHEAVDMMLSGNKLGDAGA
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQTV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLDGDKLTTSGGRVLCVVGLADSVREAQQHAYETINQINFEGMQYRRDIGFRAL
EEECCCCCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
NRKSA
CCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHH
EGVAFTLVGPEAPLAAGIVNLFRARGLKVFGPTREAAQLESSKDFAKAFMKRHGIPTAEY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDVAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHEAVDMMLSGNKLGDAGA
HHHHHHHHHHHEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKEGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQTV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLDGDKLTTSGGRVLCVVGLADSVREAQQHAYETINQINFEGMQYRRDIGFRAL
EEECCCCCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHH
NRKSA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]