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The map label for this gene is xthA [H]

Identifier: 78066923

GI number: 78066923

Start: 2531390

End: 2532166

Strand: Direct

Name: xthA [H]

Synonym: Bcep18194_A5454

Alternate gene names: 78066923

Gene position: 2531390-2532166 (Clockwise)

Preceding gene: 78066918

Following gene: 78066928

Centisome position: 68.52

GC content: 66.15

Gene sequence:

>777_bases
ATGAAAATCGCCACCTGGAACGTCAACTCGCTCAACGTCCGCAAGCAGCATGTGCTCGACTGGCTCGCGCAAAGCGGAAC
CGACGTGCTGTGCCTGCAGGAACTGAAGCTGCCGGACGAGAAATTTCCGCGCGCCGAGCTTGAAGCCGCCGGCTACCGCA
GCTGGTTCACGGGCCAGAAGACCTACAACGGCGTCGCGATCCTCGCGCGCGACACGCTGTCCGTCGACGAATCGGATGTC
GTGCGCAACATCCCCGGTTTCGACGATCCGCAGCAGCGCGTGGTCGCCGCGACGGTCGACGGCGTGCGCATCGTGTCCGC
GTATTTCCCGAACGGCCAGGCGCCCGACTCCGACAAGTTCGTCTACAAGATGCAATGGCTCGACGCGCTGCAGGCATGGC
TGCGCACCGAGCTGCAGCGCTACCCGAAGCTCGCGCTGCTCGGCGACTACAACATCGCACCGGAAGACCGCGACGTGCAC
GACCCGGCGAAATGGGAAGGCCAGAACCTCGTGTCGCCGCAGGAGCGCGCCCACTTCGCGCAGCTGATCGAGCTCGGCTT
CGTCGATGCGTTCCGCCGCTTCGAACAGCCGGAGAAGACCTTCACGTGGTGGGACTACCGGATGATGGCGTTCCGCCGCA
ACGCGGGGCTGCGCATCGACCACGTGCTGCTGTCGCCGGCGCTCGCCGAGACGTGCACGTCGTGCGAAGTCGATCGCACG
CCGCGCACGTGGGAACAGCCGTCCGACCACACGCCCGTCGTCGCGGTCGTCGGCTGA

Upstream 100 bases:

>100_bases
GCGCGGCGCCTCCCCCGCCCCGCACGAAGCGCGCGTTCGCGCCGCGTGCTCGCGGTAAGATGGCGGCCTTCGCATTCACC
CCGCACGCACACTCCCGTTC

Downstream 100 bases:

>100_bases
TCGCTCGATCCGCCCGCGCGCGTTCAGCGCCGCGCGGGCGCCGGCCCGAGATGGGCCCACAGGAACCCGAATTCGGCCGC
ATCGGCTTCCGCGCGTTCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRAELEAAGYRSWFTGQKTYNGVAILARDTLSVDESDV
VRNIPGFDDPQQRVVAATVDGVRIVSAYFPNGQAPDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHVLLSPALAETCTSCEVDRT
PRTWEQPSDHTPVVAVVG

Sequences:

>Translated_258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRAELEAAGYRSWFTGQKTYNGVAILARDTLSVDESDV
VRNIPGFDDPQQRVVAATVDGVRIVSAYFPNGQAPDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHVLLSPALAETCTSCEVDRT
PRTWEQPSDHTPVVAVVG
>Mature_258_residues
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRAELEAAGYRSWFTGQKTYNGVAILARDTLSVDESDV
VRNIPGFDDPQQRVVAATVDGVRIVSAYFPNGQAPDSDKFVYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHVLLSPALAETCTSCEVDRT
PRTWEQPSDHTPVVAVVG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=30.6818181818182, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=30.6818181818182, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=30.6818181818182, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=36.4312267657993, Blast_Score=159, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221330655, Length=260, Percent_Identity=27.6923076923077, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136678, Length=260, Percent_Identity=27.6923076923077, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29536; Mature: 29536

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRAELEAAGYRSWFTGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHHHCCCHHHCCCCC
TYNGVAILARDTLSVDESDVVRNIPGFDDPQQRVVAATVDGVRIVSAYFPNGQAPDSDKF
CCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCCCCCCCCCE
VYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
EHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHVLLSPALAETCTSCEVDRT
HHHHHHHHHHHHHHCCCCCCEEHHHHHEEEHHHCCCCEEHHHHHHHHHHHHHHCCCCCCC
PRTWEQPSDHTPVVAVVG
CCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKIATWNVNSLNVRKQHVLDWLAQSGTDVLCLQELKLPDEKFPRAELEAAGYRSWFTGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHHHCCCHHHCCCCC
TYNGVAILARDTLSVDESDVVRNIPGFDDPQQRVVAATVDGVRIVSAYFPNGQAPDSDKF
CCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCCCCCCCCCE
VYKMQWLDALQAWLRTELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
EHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLIELGFVDAFRRFEQPEKTFTWWDYRMMAFRRNAGLRIDHVLLSPALAETCTSCEVDRT
HHHHHHHHHHHHHHCCCCCCEEHHHHHEEEHHHCCCCEEHHHHHHHHHHHHHHCCCCCCC
PRTWEQPSDHTPVVAVVG
CCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]