Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 78066915

GI number: 78066915

Start: 2519658

End: 2520518

Strand: Direct

Name: folD [H]

Synonym: Bcep18194_A5446

Alternate gene names: 78066915

Gene position: 2519658-2520518 (Clockwise)

Preceding gene: 78066914

Following gene: 78066916

Centisome position: 68.21

GC content: 67.48

Gene sequence:

>861_bases
ATGACAGCCCTCCTCATCGACGGCAACGCCCTTTCGAAGACCCTGCGCGCACAGGCCGCCGAACGCGCCGCCGCCCTGAC
CGCACGCGGCCACCAGCCCGGTCTCGCGGTGATCCTCGTCGGCGCCAACCCGGCGAGCGAAGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCTGAAGGACGCGTACCCGGACACGCTGTCGGAGGCCGACCTGCTCGCG
CGCATCGACGAGCTGAACCGCGATCCGAAGATCCACGGCATCCTCGTCCAGCTCCCGCTGCCCAAGCATATCGACAGCCA
CAAGGTGATCGAGGCGATCGCGCCGGAGAAGGACGTCGACGGCTTCCACATCGCGAACGCCGGCGCGCTGATGACCGGCA
AGCCGCTGTTCCGCCCGTGCACGCCGTATGGCGTGATGAAGATGTTCGAGGCACATGACATTCCGCTGCAGGGTGCGAAC
GCGGTCGTGATCGGTCGCTCGAACATCGTCGGCAAGCCGATGGCGATGATGCTGCTCGACGCCGGCGCGACCGTGACGAT
CTGCCACAGCAAGACGCGTGACCTCGCCGCGCACACGCGGCAGGCCGACATCGTGGTCGCCGCGGTCGGCAAGCGCAACA
TCCTGACCGCCGACATGGTGAAGCCCGGCGCGACGGTGATCGACGTCGGCATGAACCGCGACGACGCCGGCAAGCTGTGC
GGCGACGTCGACTTCGCGGGTGTGAAGGAAGTCGCCAGCCACATCACGCCGGTGCCGGGCGGTGTCGGCCCGATGACCAT
CACGATGCTGCTGATCAACACGATCGAAGCCGCCGAGCGCGCCGCCGACGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTGTCGGGCGGCAGCAGGCGAACGGTATAATCGCGTGCTTTGCTGCGACGCTTCGGGCGCCGCACGACCGCATTCCAAC
TTCCCGGCAGGACCACCACC

Downstream 100 bases:

>100_bases
GCGCCAGCCCATTCAGCCGGCGTGCGATTCCGCGCGCCGGCGCCTCGCCGGCCACACCGCACCGCGTCGGCCGCGCCGTC
AAACGCCTTCCCCCCGCATT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPDTLSEADLLA
RIDELNRDPKIHGILVQLPLPKHIDSHKVIEAIAPEKDVDGFHIANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGAN
AVVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVASHITPVPGGVGPMTITMLLINTIEAAERAADAAA

Sequences:

>Translated_286_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPDTLSEADLLA
RIDELNRDPKIHGILVQLPLPKHIDSHKVIEAIAPEKDVDGFHIANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGAN
AVVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVASHITPVPGGVGPMTITMLLINTIEAAERAADAAA
>Mature_285_residues
TALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPDTLSEADLLAR
IDELNRDPKIHGILVQLPLPKHIDSHKVIEAIAPEKDVDGFHIANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANA
VVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLCG
DVDFAGVKEVASHITPVPGGVGPMTITMLLINTIEAAERAADAAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=46.2585034013605, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.578231292517, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.4137931034483, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=26.9058295964126, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=51.7605633802817, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=42.3728813559322, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=47.5524475524476, Blast_Score=265, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=306, Percent_Identity=40.5228758169935, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=25.7627118644068, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=47.4747474747475, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=47.4747474747475, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=44.636678200692, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=44.636678200692, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=44.636678200692, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=44.636678200692, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30088; Mature: 29957

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHCCCEECCC
GFFSLKDAYPDTLSEADLLARIDELNRDPKIHGILVQLPLPKHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHIANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANAVVIGRSNIVGKPMAMMLLD
CEEEECCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEC
AGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCHHHHC
GDVDFAGVKEVASHITPVPGGVGPMTITMLLINTIEAAERAADAAA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHCCCEECCC
GFFSLKDAYPDTLSEADLLARIDELNRDPKIHGILVQLPLPKHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHIANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANAVVIGRSNIVGKPMAMMLLD
CEEEECCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEC
AGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCHHHHC
GDVDFAGVKEVASHITPVPGGVGPMTITMLLINTIEAAERAADAAA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA