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The map label for this gene is tig

Identifier: 78066694

GI number: 78066694

Start: 2280448

End: 2281794

Strand: Reverse

Name: tig

Synonym: Bcep18194_A5225

Alternate gene names: 78066694

Gene position: 2281794-2280448 (Counterclockwise)

Preceding gene: 78066697

Following gene: 78066693

Centisome position: 61.77

GC content: 62.73

Gene sequence:

>1347_bases
ATGGCTAACGTTGTTGAGAACCTCGGCAAGCTTGAACGCCGCGTGACGATTTCCCTGCCGAAAGACACCGTGCAGAAGGA
AATCGACGCCCGTATCCAGAAACTCGCGAAGACCGTGCGCATGCCGGGTTTCCGCCCGGGCAAGGTGCCGGTGAAGATGG
TCGCGCAACAGTACGCGGGTCAGGTCGAAGCAGAAGTGCTGAGCGACAAGATCGGCCAGGAATTCTTCACGATCAGCCGC
GCGGAAAACCTGCGCGTCGCTGGCCAGCCGAGCTTCGAGCCGAAGCAGGAGCAGGCTGAAGGTGCATACGCGTTCGACGC
GACGTTCGAGGTCTACCCGGAAGTCAAGATCGGCGATCTGGCGACGGCTGAAGTCGAGCGCTCGACGACGTCGATCGGCG
ACGCCGAAATCGACCGTACGCTGGACATCCTGCGCAAGCAGCGCGTGCACTTCCACGCACGCGGCGAAGCCGGCGAACAC
GGCGACGGCGGTGCGGACACGGCAGCCAAGAACGGCGACCGCGTGACGGTCGACTTCGTCGGCAAGATCGACGACGTCGC
GTTCCAGGGCGGCACGGCTGAAGATTTCCCGTTCGTGCTCGGCGAAGGCCGCATGCTGCCGGAATTCGAAACCGCGGCAC
TGGGCCTGAAGGTCGGTGAATCGCGCACGTTCGACCTGGCGTTCCCGGAGGACTACCACGGCAAGGACGTCGCGGGCAAG
ACGGCGCAATTCACGGTCACGATGAAGAAGATCGAGTGGCCGCACCTGCCGGAAATCGACGGTGAATTCGCGAAGTCGCT
CGGCATCGAAGACGGCGATCTCACGAAGATGCGCGGCGAGATCAAGGACAACCTCGAGCGTGAAGCGAAGCGCCGCACGC
AGTCGATCGTCAAGAACCAGGTGATGGACGCGCTGCTGAAGATCTCCGAACTCGACGTGCCGAAGGCGCTGATCGAGCAG
GATCAGCAACGCCTCGTCGAAATGGCTCGCCAGGATCTGGCGCAGCGCGGCGTGCCGAACGCGAAGGACGCACCGATCCC
GGCCGAGATGTTCACCGAGCAGGCAGAACGCCGCGTGAAGCTGGGCCTCGTGCTGGCCGAGCTGGTGAAGTCGAACGGCC
TCGAAGCGAAGCCGGAACAGATCCGCGCGGAAGTCGACGAGTTCGCGAAGAGCTACGAAGACCCGAAGGAAGTGGTCCGC
TGGTATTATTCGAACCAGCAGCGCCTCGCCGAGATGGAAGCGTTCGTCGTTGAAAGCAACGTCGTCGATTTCGTGCTGGG
CAAGGCGAAGGTGACGGACAAGGAAGTGAGCTTCGAAGCACTCGCGAGCGCATCGGCGCAAGCGTAA

Upstream 100 bases:

>100_bases
GCGCTCCGGCGCGGCGAGTGGATGTGACGCACATCACGCCGCAGGCAGGCACGGCAAGGAAAACCCGATTCGCTCGAATA
ATTTTAGGACGATTGAAGCC

Downstream 100 bases:

>100_bases
GTGTTGCTCTAGCGGCGTGCCGGCTGTCGGAGCGACGGCCCGCACGCCGTTTTTACGTCAAGCGCACGGTTGCCATTAAT
ATGGCGATTGAGCGGGCGTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MANVVENLGKLERRVTISLPKDTVQKEIDARIQKLAKTVRMPGFRPGKVPVKMVAQQYAGQVEAEVLSDKIGQEFFTISR
AENLRVAGQPSFEPKQEQAEGAYAFDATFEVYPEVKIGDLATAEVERSTTSIGDAEIDRTLDILRKQRVHFHARGEAGEH
GDGGADTAAKNGDRVTVDFVGKIDDVAFQGGTAEDFPFVLGEGRMLPEFETAALGLKVGESRTFDLAFPEDYHGKDVAGK
TAQFTVTMKKIEWPHLPEIDGEFAKSLGIEDGDLTKMRGEIKDNLEREAKRRTQSIVKNQVMDALLKISELDVPKALIEQ
DQQRLVEMARQDLAQRGVPNAKDAPIPAEMFTEQAERRVKLGLVLAELVKSNGLEAKPEQIRAEVDEFAKSYEDPKEVVR
WYYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASASAQA

Sequences:

>Translated_448_residues
MANVVENLGKLERRVTISLPKDTVQKEIDARIQKLAKTVRMPGFRPGKVPVKMVAQQYAGQVEAEVLSDKIGQEFFTISR
AENLRVAGQPSFEPKQEQAEGAYAFDATFEVYPEVKIGDLATAEVERSTTSIGDAEIDRTLDILRKQRVHFHARGEAGEH
GDGGADTAAKNGDRVTVDFVGKIDDVAFQGGTAEDFPFVLGEGRMLPEFETAALGLKVGESRTFDLAFPEDYHGKDVAGK
TAQFTVTMKKIEWPHLPEIDGEFAKSLGIEDGDLTKMRGEIKDNLEREAKRRTQSIVKNQVMDALLKISELDVPKALIEQ
DQQRLVEMARQDLAQRGVPNAKDAPIPAEMFTEQAERRVKLGLVLAELVKSNGLEAKPEQIRAEVDEFAKSYEDPKEVVR
WYYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASASAQA
>Mature_447_residues
ANVVENLGKLERRVTISLPKDTVQKEIDARIQKLAKTVRMPGFRPGKVPVKMVAQQYAGQVEAEVLSDKIGQEFFTISRA
ENLRVAGQPSFEPKQEQAEGAYAFDATFEVYPEVKIGDLATAEVERSTTSIGDAEIDRTLDILRKQRVHFHARGEAGEHG
DGGADTAAKNGDRVTVDFVGKIDDVAFQGGTAEDFPFVLGEGRMLPEFETAALGLKVGESRTFDLAFPEDYHGKDVAGKT
AQFTVTMKKIEWPHLPEIDGEFAKSLGIEDGDLTKMRGEIKDNLEREAKRRTQSIVKNQVMDALLKISELDVPKALIEQD
QQRLVEMARQDLAQRGVPNAKDAPIPAEMFTEQAERRVKLGLVLAELVKSNGLEAKPEQIRAEVDEFAKSYEDPKEVVRW
YYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASASAQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=443, Percent_Identity=39.0519187358916, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 49674; Mature: 49542

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVENLGKLERRVTISLPKDTVQKEIDARIQKLAKTVRMPGFRPGKVPVKMVAQQYAG
CCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
QVEAEVLSDKIGQEFFTISRAENLRVAGQPSFEPKQEQAEGAYAFDATFEVYPEVKIGDL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCEEEECEEEECCCEEECCC
ATAEVERSTTSIGDAEIDRTLDILRKQRVHFHARGEAGEHGDGGADTAAKNGDRVTVDFV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHCCCCCEEEEEEC
GKIDDVAFQGGTAEDFPFVLGEGRMLPEFETAALGLKVGESRTFDLAFPEDYHGKDVAGK
CCHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHEEEECCCCEEEEECCCCCCCCCCCCC
TAQFTVTMKKIEWPHLPEIDGEFAKSLGIEDGDLTKMRGEIKDNLEREAKRRTQSIVKNQ
CEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDALLKISELDVPKALIEQDQQRLVEMARQDLAQRGVPNAKDAPIPAEMFTEQAERRVK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGLVLAELVKSNGLEAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAFVVESN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
VVDFVLGKAKVTDKEVSFEALASASAQA
HHHHHHCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANVVENLGKLERRVTISLPKDTVQKEIDARIQKLAKTVRMPGFRPGKVPVKMVAQQYAG
CHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
QVEAEVLSDKIGQEFFTISRAENLRVAGQPSFEPKQEQAEGAYAFDATFEVYPEVKIGDL
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCEEEECEEEECCCEEECCC
ATAEVERSTTSIGDAEIDRTLDILRKQRVHFHARGEAGEHGDGGADTAAKNGDRVTVDFV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHCCCCCEEEEEEC
GKIDDVAFQGGTAEDFPFVLGEGRMLPEFETAALGLKVGESRTFDLAFPEDYHGKDVAGK
CCHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHEEEECCCCEEEEECCCCCCCCCCCCC
TAQFTVTMKKIEWPHLPEIDGEFAKSLGIEDGDLTKMRGEIKDNLEREAKRRTQSIVKNQ
CEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDALLKISELDVPKALIEQDQQRLVEMARQDLAQRGVPNAKDAPIPAEMFTEQAERRVK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGLVLAELVKSNGLEAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAFVVESN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
VVDFVLGKAKVTDKEVSFEALASASAQA
HHHHHHCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA